LOCUS       sequence-328036-        8753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HLA-A*24:02 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: HLA_A2402
FEATURES             Location/Qualifiers
     source          1..8753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      606..615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1760..2348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1813..1833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2231..2242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2420..2653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2731..2852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2768..2787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2822..2841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3047..3066)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3048..3066)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3076..3093)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3076..3092)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3085..3107)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3234..3689
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3321..3340)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3531..3552
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3715..3819
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3820..3888,3889..4680)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4038..4057)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4851..5439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5340..5359
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5593..5610
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5727..5748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5763..5768,5769..5786,5787..5793)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5801..5817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5806..5828
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5825..5841
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5825..5841
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5860..5880
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5862..5880
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5908..6134
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6135..6315
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6362..6487
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6980..7213
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7398..7442
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7617..8117
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8036..8054
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8129..8524
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8584..8701
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtcgccac catggccgtc atggcgcccc gaaccctcgt cctgctactc tcgggggccc
      661 tggccctgac ccagacctgg gcaggctccc actccatgag gtatttctcc acatccgtgt
      721 cccggcccgg ccgcggggag ccccgcttca tcgccgtggg ctacgtggac gacacgcagt
      781 tcgtgcggtt cgacagcgac gccgcgagcc agaggatgga gccgcgggcg ccgtggatag
      841 agcaggaggg gccggagtat tgggacgagg agacagggaa agtgaaggcc cactcacaga
      901 ctgaccgaga gaacctgcgg atcgcgctcc gctactacaa ccagagcgag gccggttctc
      961 acaccctcca gatgatgttt ggctgcgacg tggggtcgga cgggcgcttc ctccgcgggt
     1021 accaccagta cgcctacgac ggcaaggatt acatcgccct gaaagaggac ctgcgctctt
     1081 ggaccgcggc ggacatggcg gctcagatca ccaagcgcaa gtgggaggcg gcccatgtgg
     1141 cggagcagca gagagcctac ctggagggca cgtgcgtgga cgggctccgc agatacctgg
     1201 agaacgggaa ggagacgctg cagcgcacgg acccccccaa gacacatatg acccaccacc
     1261 ccatctctga ccatgaggcc actctgagat gctgggccct gggcttctac cctgcggaga
     1321 tcacactgac ctggcagcgg gatggggagg accagaccca ggacacggag cttgtggaga
     1381 ccaggcctgc aggggatgga accttccaga agtgggcagc tgtggtggta ccttctggag
     1441 aggagcagag atacacctgc catgtgcagc atgagggtct gcccaaaccc ctcaccctga
     1501 gatgggagcc atcttcccag cccaccgtcc ccatcgtggg catcattgct ggcctggttc
     1561 tccttggagc tgtgatcact ggagctgtgg tcgctgctgt gatgtggagg aggaacagct
     1621 cagatagaaa aggagggagc tactctcagg ctgcaagcag tgacagtgcc cagggctctg
     1681 atgtgtctct cacagcttgt aaagtgtgaa aagtggttta gtaatgagct agcgctaacc
     1741 ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     1801 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     1861 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     1921 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     1981 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2041 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2101 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2161 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2221 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2281 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2341 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2401 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     2461 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2521 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2581 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2641 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     2701 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     2761 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2821 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3061 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3121 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3181 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3241 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3301 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3361 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3421 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3481 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3541 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3601 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3661 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     3721 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3781 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3841 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3901 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3961 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4021 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4081 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4141 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4201 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4261 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4321 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4381 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4441 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4501 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4561 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4621 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4681 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4741 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4801 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4861 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4921 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4981 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5041 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5101 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5161 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5221 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5281 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5341 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5401 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5461 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5521 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5581 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     5641 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     5701 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     5761 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     5821 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     5881 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     5941 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     6001 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     6061 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     6121 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6181 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6241 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6301 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6361 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6421 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6481 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6541 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6601 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6661 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6721 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6781 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     6841 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     6901 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     6961 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7021 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7081 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7141 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7201 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7261 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     7321 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     7381 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     7441 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     7501 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     7561 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccttgc
     7621 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     7681 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     7741 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     7801 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     7861 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     7921 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     7981 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     8041 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     8101 ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag aagaatccac
     8161 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     8221 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     8281 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     8341 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     8401 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     8461 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     8521 gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag tattcatcca
     8581 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8641 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     8701 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg ggg
//
