LOCUS       sequence-328038-        8688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of candidate TCR #5 TCRalpha cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CMVTCRa_candidate5
FEATURES             Location/Qualifiers
     source          1..8688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1499..2087
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1552..1572)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1970..1981)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2159..2392
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2470..2591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2507..2526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2561..2580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2631..2766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2786..2805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2787..2805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2815..2832)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2815..2831)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2824..2846)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2973..3428
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3060..3079)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3270..3291
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3454..3558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3559..3627,3628..4419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3777..3796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4590..5178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5079..5098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5332..5349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5466..5487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5502..5507,5508..5525,5526..5532)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5540..5556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5545..5567
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5564..5580
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5564..5580
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5599..5619
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5601..5619
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5647..5873
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5874..6054
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6101..6226
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6719..6952
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7137..7181
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7356..7856
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7775..7793
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7878..8477
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7878..7897)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8374..8394
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8519..8636
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgccgccac catgctgact gccagcctgt tgagggcagt catagcctcc atctgtgttg
      661 tatccagcat ggctcagaag gtaactcaag cgcaaactga aatttctgtg gtggagaagg
      721 aggatgtgac cttggactgt gtgtatgaaa cccgtgatac tacttattac ttattctggt
      781 acaagcaacc accaagtgga gaattggttt tccttattcg tcggaactct tttgatgagc
      841 aaaatgaaat aagtggtcgg tattcttgga acttccagaa atccaccagt tccttcaact
      901 tcaccatcac agcctcacaa gtcgtggact cagcagtata cttctgtgct ctgagtgagg
      961 gcttccaaac tggggcaaac aacctcttct ttgggactgg aacgagactc accgttattc
     1021 cctatatcca gaaccctgac cctgccgtgt accagctgag agactctaaa tccagtgaca
     1081 agtctgtctg cctattcacc gattttgatt ctcaaacaaa tgtgtcacaa agtaaggatt
     1141 ctgatgtgta tatcacagac aaatgtgtgc tagacatgag gtctatggat tttaaatcta
     1201 attcagcagt agcctggagc aacaaatctg actttgcatg tgcaaacgcc ttcaacaaca
     1261 gcattattcc agaagacacc ttcttcccca gcccagaaag ttcctgtgat gtcaagctgg
     1321 tcgagaaaag ctttgaaaca gatacgaacc taaactttca aaacctgtca gtgattgggt
     1381 tccgaatcct cctcctgaaa gtggccgggt ttaatctgct catgacgctg cggctgtggt
     1441 ccagctagaa agtggtttag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
     1501 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     1561 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     1621 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     1681 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     1741 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     1801 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     1861 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     1921 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     1981 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     2041 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     2101 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     2161 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     2221 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2281 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2341 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     2401 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     2461 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2521 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2581 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     2641 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2701 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2761 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     2821 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2881 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2941 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     3001 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3061 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3121 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3181 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3241 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3301 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3361 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     3421 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3481 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3661 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3721 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3781 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3841 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3901 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3961 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4021 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4081 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4141 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4201 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4261 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4321 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4381 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4441 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4501 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4561 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4621 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4681 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4741 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4801 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4861 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5161 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5221 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5281 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5341 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5401 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5461 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     5521 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     5581 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     5641 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     5701 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     5761 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     5821 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     5881 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5941 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6001 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     6061 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     6121 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     6181 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     6241 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     6301 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     6361 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     6421 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     6481 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     6541 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     6601 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     6661 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     6721 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     6781 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     6841 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     6901 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     6961 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     7021 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     7081 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     7141 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     7201 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     7261 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     7321 cgtttcagac ccacctccca accccgaggg gacccttgcg ccttttccaa ggcagccctg
     7381 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     7441 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     7501 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     7561 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     7621 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     7681 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     7741 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     7801 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     7861 taccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     7921 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     7981 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     8041 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg
     8101 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     8161 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     8221 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     8281 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     8341 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     8401 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     8461 gcaagcccgg tgcctgagaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     8521 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     8581 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     8641 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggggg
//
