LOCUS       sequence-328049-       10820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK14 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10820)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK14_IDG-K
FEATURES             Location/Qualifiers
     source          1..10820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    136..252
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    286..306
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        306..1517
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1354..1373
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1521..1541)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1542..1551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1554..2324
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2331..2372
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2373..2397
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3760..3784)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3814..4402
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3867..3887)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4285..4296)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4416..4915
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4451..4474)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4848..4867
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4936..5535
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4936..4955)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5432..5452
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5673..5906
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5978..6099
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6015..6034)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6069..6088
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6139..6274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6294..6313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6295..6313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6323..6340)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6323..6339)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6332..6354)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6481..6936
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6568..6587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6778..6799
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6962..7066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7067..7135,7136..7927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8098..8686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8587..8606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8840..8857
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8974..8995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9010..9015,9016..9033,9034..9040)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9048..9064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9053..9075
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9072..9088
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9072..9088
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9107..9127
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9109..9127
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9155..9381
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9382..9562
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9609..9734
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10227..10460
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10645..10689
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
      121 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      181 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      241 attcaaaatt ttatcgtcca gtgtggtgga attctgcaga tatcacaact ttgtatagaa
      301 aagttggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
      361 cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag
      421 gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat
      481 tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg
      541 cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg
      601 ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt
      661 tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct
      721 gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag
      781 accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg
      841 gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc
      901 tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag
      961 gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg
     1021 accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg
     1081 gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc
     1141 gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag
     1201 gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc
     1261 tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt
     1321 ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct
     1381 cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg
     1441 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
     1501 tggctttttt gttagacggc caacttttgt atacaaagtg ggccaccatg ggaatcccgc
     1561 tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca gtcctgcctg
     1621 tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag agtggggatg
     1681 cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa tggttctctc
     1741 cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa
     1801 tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag ctgggagcag
     1861 acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag ctggcaggca
     1921 tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt ggcgccggga
     1981 ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc
     2041 aggaagcagg gattaacctg gacaggaata ctctggccgc tatgctgatc cgagagctgc
     2101 gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgtca cggtgggaga
     2161 agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa gagattttcg
     2221 ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac ggagttatca
     2281 aaccctggat gggcggagaa atcagtctgc ggtctgccga aaaggctagc ggcaagccca
     2341 tccccaaccc cctgctgggc ctggacagca ccacaacttt gtacaaaaaa gcaggctcca
     2401 ccatgcacgg ttactttggc tgcaatgctg ctgcagagcc cggttactct gccttcgtgg
     2461 gaactccaca gatatgtgtc acaaagatgt ctacacggaa ctgccaggga atggactcag
     2521 tgatcaaacc cctggacaca attcctgagg ataaaaaagt cagagttcag aggacacaga
     2581 gcacttttga cccatttgag aaaccagcta atcaagtaaa gagggtgcat tctgagaaca
     2641 atgcttgcat taactttaag acctcctcca ctggcaaaga gtcacctaaa gttaggcggc
     2701 actccagccc cagctcgcca acaagtccca aatttggaaa agctgactca tatgaaaagc
     2761 tggaaaaact aggggaagga tcttatgcta cagtatacaa agggaaaagc aaggtaaatg
     2821 ggaagttggt agctctgaag gtgatcaggc tgcaggaaga agaagggaca cctttcacag
     2881 ctatcaggga agcttctctt ttaaaaggac taaaacatgc taacatagtg ctacttcatg
     2941 acatcatcca taccaaggag acgctgacac ttgtgtttga atatgtgcac actgatttat
     3001 gtcagtacat ggacaagcac cctggggggc tgcatccaga taatgtgaag ttgtttttat
     3061 ttcagttgct gcgaggtctg tcttacatcc accagcgtta tattttgcac agagacctga
     3121 aaccacagaa ccttctgatc agtgacacgg gggagttaaa gctggcagat ttcggtcttg
     3181 caagagcaaa atccgtccct agccacacat actccaacga agtggttacc ttgtggtaca
     3241 gacctccaga tgtccttcta ggctcaacag aatattccac ctgccttgac atgtggggag
     3301 taggttgcat ctttgttgaa atgatccaag gagttgctgc ttttccagga atgaaagaca
     3361 ttcaggatca acttgaacga atatttctgg ttcttggaac accaaatgag gacacatggc
     3421 ctggagttca ttctttacca cattttaagc cagaacgctt taccctgtac agctctaaaa
     3481 accttagaca agcatggaat aagctcagct atgtgaacca tgcagaggac ctggcctcca
     3541 agctcctaca atgttcccca aagaacagac tgtcggcaca ggctgccttg agccacgagt
     3601 attttagtga cctgccgcca cggctatggg aactcaccga catgtcttct atttttactg
     3661 tcccaaatgt gagattgcaa ccagaagctg gagaaagcat gcgggccttt gggaaaaaca
     3721 atagttatgg caaaagtcta tcaaacagca agcactagga cccagctttc ttgtacaaag
     3781 ttgtgatatc cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt
     3841 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3901 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3961 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4021 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4081 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4141 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4201 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4261 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4321 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4381 tccctttggg ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag
     4441 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     4501 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     4561 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     4621 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     4681 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     4741 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     4801 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     4861 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     4921 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4981 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     5041 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     5101 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     5161 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     5221 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     5281 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     5341 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     5401 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     5461 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     5521 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     5581 ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga
     5641 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
     5701 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5761 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5821 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5881 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5941 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6001 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6061 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6121 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6181 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6241 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6301 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6361 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6781 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6841 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6901 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6961 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7021 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7081 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7141 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7201 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7261 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7321 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7381 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7441 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7501 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7561 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7621 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7681 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7741 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7801 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7861 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7921 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7981 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8041 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8101 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8161 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8221 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8281 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8341 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8461 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8521 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8581 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8641 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8701 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8761 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8821 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8881 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8941 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9001 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9061 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9121 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     9181 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     9241 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     9301 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     9361 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     9421 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9481 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9541 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     9601 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     9661 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     9721 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     9781 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     9841 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     9901 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     9961 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    10021 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
    10081 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
    10141 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    10201 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    10261 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    10321 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    10381 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    10441 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    10501 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    10561 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    10621 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    10681 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    10741 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    10801 agagttaggc agggatattc
//
