LOCUS       sequence-328052-        1749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 4 terminator for golden gate assembly .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK300
FEATURES             Location/Qualifiers
     source          1..1749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             113..772
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(179..198)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      843..877
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(1092..1680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1172..1191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcatgattg atcgggcacg taagaggttc caactttcac cataatgaaa taagatcact
       61 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
      121 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      181 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      241 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      301 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
      361 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      421 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      481 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      541 tttcgtttca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      601 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      661 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
      721 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatttgatat
      781 cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
      841 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tctggtctca cagctgacaa
      901 atgctctttc cctaaactcc ccccataaaa aaacccgccg aagcgggttt ttacgttatt
      961 tgcggattaa cgattacgga ttgagaccga ctacggttat ccacagaatc aggggataac
     1021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1501 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1741 gggattttg
//
