LOCUS       sequence-328077-       10709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for FLCN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, 
            Lim CY, Samelson AJ, Hurley JH, Zoncu R
  TITLE     Structural mechanism of a Rag GTPase activation checkpoint by the 
            lysosomal folliculin complex.
  JOURNAL   Science. 2019 Nov 22;366(6468):971-977. doi: 
            10.1126/science.aax0364. Epub 2019 Oct 31.
  PUBMED    31672913
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax0364"; journalName: "Science"; date: 
            "2019-11-22- 22"; volume: "366"; issue: "6468"; pages: "971-977"
COMMENT     Sequence Label: pLJM1 FLCN (F118D) FLAG GFP
FEATURES             Location/Qualifiers
     source          1..10709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(140..159)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     487..508
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    595..712
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        761..1271
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1190..1208
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1293..1892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1293..1312)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1789..1809
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2020..2253
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2325..2446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2362..2381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2416..2435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2486..2621
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2641..2660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2642..2660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2670..2687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2670..2686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2679..2701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2828..3283
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2915..2934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3125..3146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3309..3413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3414..3482,3483..4274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3632..3651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4445..5033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4934..4953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5187..5204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5321..5342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5357..5362,5363..5380,5381..5387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5395..5411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5400..5422
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5419..5435
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5419..5435
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5454..5474
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5456..5474
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5502..5728
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5729..5909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5956..6081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6574..6807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6992..7036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     7334..7354
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        7525..8736
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8573..8592
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             10498..10521
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(10546..10548,10549..10709,1..553)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(10546..10548,10549..10709,1..550)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(10588..10609)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
       61 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      121 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      181 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      241 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      301 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      361 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      421 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      481 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      541 gctgtacaag tgagaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
      601 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      661 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
      721 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
      781 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
      841 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
      901 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
      961 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
     1021 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     1081 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     1141 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     1201 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     1261 tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
     1321 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     1381 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     1441 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     1501 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     1561 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     1621 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     1681 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     1741 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     1801 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     1861 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     1921 ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag
     1981 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     2041 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     2101 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     2161 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     2221 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     2281 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     2341 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2401 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     2461 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     2521 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     2581 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     2641 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     2701 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2761 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2821 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2881 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2941 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3001 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3061 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3121 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3181 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3241 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     3301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4561 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5161 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5221 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     5281 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     5341 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     5401 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     5461 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     5521 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     5581 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     5641 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     5701 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     5761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5881 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     5941 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     6001 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     6061 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     6121 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     6181 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     6241 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     6301 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     6361 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     6421 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     6481 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     6541 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     6601 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     6661 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     6721 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     6781 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     6841 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     6901 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     6961 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     7021 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     7081 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     7141 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     7201 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     7261 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc
     7321 cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     7381 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
     7441 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     7501 aatggactat catatgggtg cagcggcctc cgcgccgggt tttggcgcct cccgcgggcg
     7561 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     7621 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     7681 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     7741 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     7801 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     7861 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     7921 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     7981 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     8041 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     8101 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     8161 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     8221 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     8281 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     8341 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     8401 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     8461 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     8521 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     8581 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     8641 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     8701 gctaaattct ggccgttttt ggcttttttg ttagacgcta gcgctaccgg tgccaccatg
     8761 atgaacgcca tcgtggccct gtgccacttc tgtgagctgc acggcccacg gaccctgttt
     8821 tgcacagagg tgctgcacgc accactgcca cagggcgacg gaaacgagga tagcccagga
     8881 cagggagagc aggcagagga ggaggagggc ggcatccaga tgaatagccg gatgagagcc
     8941 cactccccag cagagggagc ctctgtggag agctcctctc ctggcccaaa gaagagcgac
     9001 atgtgcgagg gatgtcggtc cctggccgca ggacaccctg gctatatctc tcacgataag
     9061 gagacaagca tcaagtacgt gtctcaccag caccccagcc accctcagct ggactccatc
     9121 gtgcggcagg cctgcgtgag atccctgtct tgcgaggtgt gcccaggaag agagggacct
     9181 atcttctttg gcgacgagca gcacggcttc gtgttttctc acaccttctt tatcaaggat
     9241 agcctggcca ggggcttcca gcggtggtat tccatcatca caatcatgat ggaccggatc
     9301 tacctgatca actcttggcc ctttctgctg ggcaaggtga gaggcatcat cgacgagctg
     9361 cagggcaagg ccctgaaggt gttcgaggcc gagcagtttg gctgtccaca gagggcccag
     9421 aggatgaaca ccgccttcac accttttctg caccagagga acggcaatgc cgcccgctct
     9481 ctgaccagcc tgacatccga cgataatctg tgggcctgcc tgcacaccag ctttgcctgg
     9541 ctgctgaagg cctgtggctc caggctgaca gagaagctgc tggagggcgc ccctaccgag
     9601 gacacactgg tgcagatgga gaagctggcc gacctggagg aggagagcga gtcctgggat
     9661 aatagcgagg cagaagagga ggagaaggca ccagtgctgc cagagtccac cgagggaagg
     9721 gagctgacac agggaccagc cgagagctcc tctctgtctg gctgcggcag ctggcagcct
     9781 agaaagctgc cagtgttcaa gtctctgagg cacatgagac aggtgctggg cgcccccagc
     9841 tttaggatgc tggcctggca cgtgctgatg ggcaaccagg tcatctggaa gtcccgcgac
     9901 gtggatctgg tgcagtctgc cttcgaggtg ctgaggacca tgctgccagt gggctgcgtg
     9961 cgcatcatcc cctacagctc ccagtatgag gaggcctaca ggtgtaattt cctgggcctg
    10021 agccctcacg tgcagatccc acctcacgtg ctgtctagcg agtttgccgt gatcgtggag
    10081 gtgcacgccg ccgcccggag caccctgcac ccagtgggct gtgaggacga tcagtctctg
    10141 agcaagtatg agtttgtggt gacatccggc tctcctgtgg cagcagacag ggtgggccca
    10201 accatcctga acaagatcga ggccgccctg acaaaccaga atctgagcgt ggacgtggtg
    10261 gatcagtgcc tggtgtgcct gaaggaggag tggatgaata aggtgaaggt gctgttcaag
    10321 tttaccaagg tggactcccg ccctaaggag gatacacaga agctgctgtc catcctgggc
    10381 gcctctgagg aggataacgt gaagctgctg aagttctgga tgaccggcct gagcaagaca
    10441 tacaagtccc acctgatgtc taccgtgcgg agcccaacag ccagcgagtc cagaaatgac
    10501 tacaaagacg atgacgacaa gggtagcggt agtggaagcg tcgacgtgag caagggcgag
    10561 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
    10621 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
    10681 ttcatctgca ccaccggcaa gctgcccgt
//
