LOCUS       sequence-328078-       12643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for NPC1 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12643)
  AUTHORS   Davis OB, Shin HR, Lim CY, Wu EY, Kukurugya M, Maher CF, Perera RM, 
            Ordonez MP, Zoncu R
  TITLE     NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle 
            Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.
  JOURNAL   Dev Cell. 2020 Dec 7. pii: S1534-5807(20)30925-4. doi: 
            10.1016/j.devcel.2020.11.016.
  PUBMED    33308480
REFERENCE   2  (bases 1 to 12643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2020 Dec 7. pii: S1534-5807(20)30925-4. doi: 
            10.1016/j.devcel.2020.11.016."
COMMENT     Sequence Label: pLVX-NPC1(P202A/F203A)-FLAG
FEATURES             Location/Qualifiers
     source          1..12643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3..502)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(51..70)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     444..467
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(532..555)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4461..5643)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4470..5412
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4493..4513)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5837..5952
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(6215..6259)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6444..6677
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7174..7299
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7346..7979
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8147..8281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8171..8190)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8225..8244
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8329..8433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8434..8502,8503..9294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8652..8671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9465..10053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9954..9973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10207..10224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10341..10362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10377..10382,10383..10400,10401..10407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10415..10431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10420..10442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10439..10455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10439..10455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             10584..11217
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11424..12012
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             11530..11541
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     11939..11959
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12026..12625)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12109..12129)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     12606..12625
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc
       61 gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc
      121 ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa
      181 agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc
      241 atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc
      301 tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca
      361 ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg
      421 ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt
      481 gggaaaagcg cctcccctac ccggtagaat tatctagatg agcccgggtt atttatcatc
      541 gtcatccttg tagtcaaagt tcagcagtct ctctcgctca gtccccttgt atctttcctc
      601 agtggcgcag ctttttgcct tgttcacgct agggccgata tatgacagca ggacgggcag
      661 gaaaatcagc ccgtgtgttg cgcccagcag caccatagcc aggtacatcc ggaaataaaa
      721 gatctggaag atctggctct tagcaaaggc caggaccacg attcccccga actttgtcag
      781 ggtaatgcca ctaaacactg aacttcccat gtgtgccaga gcttcctctg ctctttccac
      841 ccggcttccc ttcattgaga cggtgaaagc tctagtgata tggctacaaa actccacact
      901 aatcccgcaa gacatgacca gattcaccag actgacggcg ttcagagaga tcccccacag
      961 ccacatcacg ccgaacatat tcaccaggac catagcgatg gtggcgcaca taatcacggc
     1021 agaccacagc tcgcagccca gcagcaccat agtgaccagg aagatagctc ccagggacac
     1081 ccccaggttg aagatggtat cgtcaatgat agtcaggtac tgttcataga acacgtaaaa
     1141 gacagagtag ggaaacactc gataggcgga tccattgatc cccatagtct ctgtcacgtt
     1201 gctagcgatc aggcgggctt tcttcagtgc atcaatgaag tcagctgagg tctgcagcac
     1261 agtgtggtat gtcatgaagt atgtggctcc cactcgggtc ccatgtccca gcaggatgtt
     1321 gactgcagag gagtatgcag cgtggcctcc tttgccacac ttgggatttg ggttgtcgga
     1381 caggaacata ggcaggaatc tcatgaagtc gcctccctgt ggtcgctgtt tgccctctgg
     1441 agtcagtggc ctacatctca cgcaggcagg gtcgaccacg cttgcattgc aaaactgatc
     1501 tgtgatgttg tccacgcgac agcagcttga ctgaggcttg acccaatcga agtaatcgtc
     1561 gatccaacta gagggggcaa agccaatcct tgtataattg tccagctggg ctgcgttgaa
     1621 gatctgctgc accagggaat cattgttaca tcccattccc ccgcacacca tattctgccc
     1681 cttggagcta gtgtagtcat ggccttcctc cagcacgaag tagacagggg ggccggcgtg
     1741 cagatactga ctgatagatt taaagtaatc caccatgtaa gaatcgtcgg gcatggacag
     1801 gctctgatcc aggccgatgt cgaccttgtt cagcacggca attgagaaac tcagcactcc
     1861 gacaaagatt gcaatcacga tggggcgcat ccagtctttc agcagcagtg gagaatagct
     1921 gttcttgaaa aacctgaaca gacagctttc tgaagcctgc acggatgtgc cgtcctctgc
     1981 tccccgcacg cagcagaaga tatccaggcg gtttttctcc tgcctcttaa tgtccaggcc
     2041 cagcaggctc acaaagcagg tgatctgcag caggaagtca ataaacactg ccagcccagc
     2101 gaacagtgaa aatgtgtgga ctgctggcat cacactcagg gcgcccagga aaaatgccac
     2161 ggtttcactg aatgaactca gaaacataga tggagcgacc tctcccagca cccttcccag
     2221 ctgctgatcc agtgtttcgc cctgcagtcg ctcgtcgcgc tggtatgcct gcaccagaat
     2281 gaagatattg tccactccga cagccaggac cagaaatggg atcacttcaa tgacgatcag
     2341 ggtcagaggc agtccaatat aggagaacac ccccaggcta caagcgacag aggacagcac
     2401 aatcaggatg ccggcaattc ccaggctgac ttttgaatcc accagcaggc gcctgcagct
     2461 cttcatatgg cccagtgcca gtgagatgta caggaacatg atagcatagc taatgaccac
     2521 ggtgaacacg tcactgtcag attctcggtt cagttcgtcc tcgatgctcc gctcggctgt
     2581 gaaactaatg gtcagatttg ggtttttgta attcttcacg aagttgatga actctttctc
     2641 ccaggcctgt gctctctgca gcttctcggt atcattatag taattgttga cagggaaggt
     2701 gatgaccagg gcggtggcat tgttgtagtt ctgatcgtca tagcctccca ggaccagcca
     2761 agggaacacg ggtccgccaa atgtccccag acatggatcg tgcagcagtg aggtgtcgtt
     2821 cagacttgca ggagcccgca cgcagtacag gaaatgggtg tgatagtcgg cgtacacgaa
     2881 gaagtcgtcg cctttcttat gatccagcac ggagtggcta ttctgaaagt agttcaggac
     2941 ggacagaatg gtacaattag tgttatatgg gctcagagga gccaggcaga tatcctgcag
     3001 ggtcacagtt tcattgtcat agctagcggt aatgttctca atggcgatct gcaggtccag
     3061 cacctgatgc agaatctgga tatccagtgg agggccgaaa ggcacgtcgg ctccagaagg
     3121 atagggctgg tagatgtgtt tatcagtcag gggtgcccta atgatcagct gctctgttct
     3181 gaaaaatggt ccgaaatgct ggtcaaagta ttccttctcc agtcgagcct ggcttgatgg
     3241 ggcgctccac agatccactg gatttgtggt cactcggacg aacaccagcc cactagaaca
     3301 ggcggtgata aacaccaggc tgaagaagat cacgcatccg gggttccgca cgcagaaaga
     3361 cccccatcga gtaaacagtc tccgcaggca cccttcgaaa gcggcgctca cgggatcaca
     3421 gcaggatgcc tcgcctttgt cggatgcatt cacgctgaaa gcgatgttgg agtcaatggg
     3481 ggtgtactcg ctcacaaagt accgctttcg ataacaccac acggcgaaaa atgcgccgaa
     3541 aaagaccagc agaaaggcca tgtaagtgat ccacataatc acatacatgg cgtccagtcc
     3601 cagaattgtc catggtgctg gtggaggtgg tggctgtggt ttaggcccgc acacgataga
     3661 gcaatcctga caggagcaag gagcggtcac ttcatcgacg gactcgtcgc atcccttagt
     3721 ggcattgttc ataggctcca tcccgtggac ggggaagtcg ctaaacactg gtgtaatggt
     3781 ggcggcggcc tggccattat ccttgttaaa catgtattcg atccaattgg tagcgttaca
     3841 ggcatctgcg tcttttccgc acagcagccc cagggcctta tcattggagc ttggtgcctc
     3901 cacgtctcgg caagcattat acatagcgtt ggcgaaggac tggcccacat agtactgcag
     3961 ttctttgaca ttagtctttg tctggtttgt cacgggatcg acgtagtcct cggtggcagt
     4021 cacgttcagg aactgtgact gccttggact acaggtcagc tcgcagaaca gattcagcag
     4081 gttataaaaa caagatgggc acctggacag aaactgcaga ggcagctgca gattatcctt
     4141 cagggtctgc agctgccgca cgtcacagca caggctcacg ttgccgaaaa agaatcctgg
     4201 gcacagttcc tgcaccagat cgtagccgtc tttgggcagt ggcttagggg gtccagagta
     4261 ttcacagtta tacctcttgt ccccataggc gatgccgcac tcgccatacc acacgcaaga
     4321 ctgactgaac acctgagcgg ggcacagcag cagcagcagc agccccagag ccagccctct
     4381 agcggtcatg gtggcgaatt ccacgtggat ccaattcgaa gcttgagctc gagatctgag
     4441 tccggtagcg ctagcgatcc tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact
     4501 gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc caaggagaat
     4561 tacatcaagt gccaagctgg cctaacttca gtctccaccc actcagtgtg gggaaactcc
     4621 atcgcataaa acccctcccc ccaacctaaa gacgacgtac tccaaaagct cgagaactaa
     4681 tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac atgaagcgac ggctgaggac
     4741 ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc gctctcccga gcgccgcgtc
     4801 ctccattttg agctccctgc agcagggccg ggaagcggcc atctttccgc tcacgcaact
     4861 ggtgccgacc gggccagcct tgccgcccag ggcggggcga tacacggcgg cgcgaggcca
     4921 ggcaccagag caggccggcc agcttgagac tacccccgtc cgattctcgg tggccgcgct
     4981 cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac gggccccgtc gccgcccgcg
     5041 gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg catttacaag actatcttgc
     5101 cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga cttatcgaaa
     5161 gcagcgagac aggcgcgaag gtgccaccag attcgcacgc ggcggcccca gcgcccaagc
     5221 caggcctcaa ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct cgaactctcc
     5281 cacccacttc caacccgaag ctcgggatca agaatcacgt actgcagcca ggggcgtgga
     5341 agtaattcaa ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca
     5401 cacggcactt acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac
     5461 tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac
     5521 atcactttcc cagtttaccc cgcgccacct tctctaggca ccggttcaat tgccgacccc
     5581 tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga
     5641 gcctcacgca tgctcttctc cacctcagtg atgacgagag cgggcgggtg agggggcggg
     5701 aacgcagcga tctctgggtt ctacgttagt gggagtttaa cgacggtccc tgggattccc
     5761 caaggcaggg gcgagtcctt ttgtatgaat tactcatcga taaactggat ctctgctgtc
     5821 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
     5881 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
     5941 ttttctttta aaggcgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt
     6001 ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga
     6061 ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta
     6121 cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat
     6181 accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca
     6241 ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta
     6301 agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct
     6361 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
     6421 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
     6481 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
     6541 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
     6601 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
     6661 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
     6721 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
     6781 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cggccgcttg
     6841 ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
     6901 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
     6961 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
     7021 gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc
     7081 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
     7141 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
     7201 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
     7261 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     7321 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     7381 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     7441 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     7501 cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga
     7561 tctgagggct cgccactccc cagtcccgcc caggccacgc ctccctggaa agtccccagc
     7621 ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg aagtactccg gatgcagctc
     7681 tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc cactctaaca cttctctctc
     7741 cgggtcatcc atcccatgca ggctcacagg gtgtaacaag ctggtgttct ctcctttatt
     7801 ggcctcttct accttatctg gctcaactgg tactagcttg tagcaccatc caaaggtcag
     7861 tggatatctg acccctggcc ctggtgtgta gttctgctaa tcagggaagt agccttgtgt
     7921 gtggtagatc cacagatcaa ggatatcttg tcttctttgg gagtgaatta gcccttccaa
     7981 ctactaagtt tgtagtacat atttaacaaa tacaatttct ttaaaatgaa aataattcag
     8041 aggaatcaca ggtttagagt aaatgaaacc acaggtaatt ggcagtggta atagggtatg
     8101 gggtgggaag tttgggatga ttttggttag cttgagttat ccagttgatc cagacatgat
     8161 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     8221 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     8281 tgatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8341 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8401 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8461 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8521 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8581 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8641 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8701 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8761 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8821 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8881 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8941 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9001 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9061 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9121 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9181 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9241 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9301 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9361 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9601 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10261 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    10321 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    10381 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    10441 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg taaaacgcta
    10501 gcaatgtcaa ggcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat
    10561 aataagatga catgaactac tactgctaga gattttccac actgactaaa agggtctgag
    10621 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
    10681 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
    10741 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
    10801 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
    10861 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
    10921 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
    10981 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
    11041 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
    11101 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
    11161 ggtagatcca cagatcaagg atatcttgtc ttcgttggga gtgaattagc ccttccagtc
    11221 ccctcttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
    11281 aaggtacctg aggtgtgact ggaaaaccca cctcctcctc ctcttgtgct tctagccagg
    11341 cacaatcagc attggtagct gctgtattgc tacttgtgat tgctccatgt ttttctaggt
    11401 ctcgactgca gaattaattc caggcgggga ggcggcccaa agggagatcc gactcgtctg
    11461 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
    11521 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
    11581 ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt
    11641 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
    11701 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
    11761 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
    11821 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
    11881 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
    11941 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc
    12001 cagaggttga ttgttccaga cgcggtcagg caccgggctt gcgggtcatg caccaggtgc
    12061 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg
    12121 ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct
    12181 ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc
    12241 cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca
    12301 tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg
    12361 cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt
    12421 cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca
    12481 gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt
    12541 cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc
    12601 gcaccgtggg cttgtactcg gtcatggtaa gcttgggctg cag
//
