LOCUS       sequence-328079-       10709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for FLCN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, 
            Lim CY, Samelson AJ, Hurley JH, Zoncu R
  TITLE     Structural mechanism of a Rag GTPase activation checkpoint by the 
            lysosomal folliculin complex.
  JOURNAL   Science. 2019 Nov 22;366(6468):971-977. doi: 
            10.1126/science.aax0364. Epub 2019 Oct 31.
  PUBMED    31672913
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax0364"; journalName: "Science"; date: 
            "2019-11-22- 22"; volume: "366"; issue: "6468"; pages: "971-977"
COMMENT     Sequence Label: pLJM1 FLCN (R164A) FLAG GFP
FEATURES             Location/Qualifiers
     source          1..10709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(104..123)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(1342..1362)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(1660..1704)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1889..2122
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2615..2740
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2787..2967
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2968..3194)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(3222..3242)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3222..3240)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3261..3277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3261..3277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3274..3296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3285..3301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3309..3315,3316..3333,3334..3339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3354..3375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3492..3509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3663..4251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3743..3762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4422..5213,5214..5282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5045..5064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5283..5387)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5413..5868)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5550..5571)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5762..5781
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5995..6017
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6009..6026
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6010..6026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6036..6055
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6036..6054
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6075..6210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6250..6371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6261..6280)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6315..6334
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             6443..6676
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(6804..7403)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6887..6907)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7384..7403
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7425..7935)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7488..7506)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    7984..8101
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(join(8143..8856,8857..8859))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(8146..8856,8857..8859))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8188..8209)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8537..8556
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8796..8817
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(8884..8907)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(join(10669..10709,1..1171))
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 atttactagt ctaacactga aaattacata ttgacccaaa tgattacatt tcaaaaggtg
       61 cctaaaaaac ttcacaaaac acactcgcca accccgagcg catagttcaa aaccggagct
      121 tcagctactt aagaagatag gtacataaaa ccgaccaaag aaactgacgc ctcacttatc
      181 cctcccctca ccagaggtcc ggcgcctgtc gattcaggag agcctaccct aggcccgaac
      241 cctgcgtcct gcgacggaga aaagcctacc gcacacctac cggcaggtgg ccccaccctg
      301 cattataagc caacagaacg ggtgacgtca cgacacgacg agggcgcgcg ctcccaaagg
      361 tacgggtgca ctgcccaacg gcaccgccat aactgccgcc cccgcaacag acgacaaacc
      421 gagttctcca gtcagtgaca aacttcacgt cagggtcccc agatggtgcc ccagcccatc
      481 tcacccgaat aagagctttc ccgcattagc gaaggcctca agaccttggg ttcttgccgc
      541 ccaccatgcc ccccaccttg tttcaacgac ctcacagccc gcctcacaag cgtcttccat
      601 tcaagactcg ggaacagccg ccattttgct gcgctccccc caacccccag ttcagggcaa
      661 ccttgctcgc ggacccagac tacagccctt ggcggtctct ccacacgctt ccgtcccacc
      721 gagcggcccg gcggccacga aagccccggc cagcccagca gcccgctact caccaagtga
      781 cgatcacagc gatccacaaa caagaaccgc gacccaaatc ccggctgcga cggaactagc
      841 tgtgccacac ccggcgcgtc cttatataat catcggcgtt caccgcccca cggagatccc
      901 tccgcagaat cgccgagaag ggactacttt tcctcgcctg ttccgctctc tggaaagaaa
      961 accagtgccc tagagtcacc caagtcccgt cctaaaatgt ccttctgctg atactggggt
     1021 tctaaggccg agtcttatga gcagcgggcc gctgtcctga gcgtccgggc ggaaggatca
     1081 ggacgctcgc tgcgcccttc gtctgacgtg gcagcgctcg ccgtgaggag gggggcgccc
     1141 gcgggaggcg ccaaaacccg gcgcggaggc cgctgcaccc atatgatagt ccattttaaa
     1201 acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg
     1261 tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa cagccttgta
     1321 tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcggtgaagg gggcggccgc
     1381 tcgaggctag tctcgtgatc gataccgtcg agatccgttc actaatcgaa tggatctgtc
     1441 tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt
     1501 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
     1561 aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt
     1621 tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa
     1681 ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg
     1741 tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca
     1801 aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat
     1861 gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac
     1921 agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg
     1981 ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg
     2041 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
     2101 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
     2161 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
     2221 ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccgctt
     2281 gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg
     2341 tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg
     2401 atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac
     2461 agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg
     2521 cccatactat atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt
     2581 cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct
     2641 tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg
     2701 ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg
     2761 ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct
     2821 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     2881 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     2941 tcagatctgg tctaaccaga gagacccgtt tatgtatcga gctaggcact taaatacaat
     3001 atctctgcaa tgcggcaatt cagtggttcg tccaatccat gtcagacccg tctgttgcct
     3061 tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac ggtgcttttt
     3121 ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact acaagagtat
     3181 tgcataagac tacattaagc ttgcagctcc agcttttgtt ccctttagtg agggttaatt
     3241 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     3361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     3421 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     3481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3841 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4081 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4861 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5041 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5101 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5161 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5221 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5401 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     5461 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5521 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     5581 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     5641 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     5701 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     5761 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5821 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     5881 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     5941 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6001 cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
     6061 tgggtacgtc cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     6121 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     6181 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga tagctagagc
     6241 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     6301 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     6361 ataaacaagt tcctctcact ctctgatatt catttctttg caagttataa atactgaata
     6421 ataagatgac atgaactact actgctagag attttccaca ctgactaaaa gggtctgagg
     6481 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     6541 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     6601 atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag
     6661 tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc
     6721 ttgtaagtca ttggtcttaa aggtaccgat gcatggggtc gtgcgctcct ttcggtcggg
     6781 cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc
     6841 ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg
     6901 aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc
     6961 actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgagacgccg
     7021 acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc
     7081 tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg
     7141 aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg
     7201 tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc
     7261 tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg
     7321 gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc
     7381 accgtgggct tgtactcggt catggtaagc tccggtggat ccccctgggg agagaggtcg
     7441 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg
     7501 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc
     7561 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc
     7621 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg
     7681 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga
     7741 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg
     7801 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc
     7861 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg
     7921 cgcaacccca accccctcga gccgcggcca aagtggatct ctgctgtccc tgtaataaac
     7981 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     8041 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     8101 attgtggatg aatactgcca tttgtctcga ggtcgagaat tctcacttgt acagctcgtc
     8161 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
     8221 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
     8281 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
     8341 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
     8401 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
     8461 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
     8521 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
     8581 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
     8641 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
     8701 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
     8761 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
     8821 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacg tcgacgcttc cactaccgct
     8881 acccttgtcg tcatcgtctt tgtagtcatt tctggactcg ctggctgttg ggctccgcac
     8941 ggtagacatc aggtgggact tgtatgtctt gctcaggccg gtcatccaga acttcagcag
     9001 cttcacgtta tcctcctcag aggcgcccag gatggacagc agcttctgtg tatcctcctt
     9061 agggcgggag tccaccttgg taaacttgaa cagcaccttc accttattca tccactcctc
     9121 cttcaggcac accaggcact gatccaccac gtccacgctc agattctggt ttgtcagggc
     9181 ggcctcgatc ttgttcagga tggttgggcc caccctgtct gctgccacag gagagccgga
     9241 tgtcaccaca aactcatact tgctcagaga ctgatcgtcc tcacagccca ctgggtgcag
     9301 ggtgctccgg gcggcggcgt gcacctccac gatcacggca aactcgctag acagcacgtg
     9361 aggtgggatc tgcacgtgag ggctcaggcc caggaaatta cacctgtagg cctcctcata
     9421 ctgggagctg taggggatga tgcgcacgca gcccactggc agcatggtcc tcagcacctc
     9481 gaaggcagac tgcaccagat ccacgtcgcg ggacttccag atgacctggt tgcccatcag
     9541 cacgtgccag gccagcatcc taaagctggg ggcgcccagc acctgtctca tgtgcctcag
     9601 agacttgaac actggcagct ttctaggctg ccagctgccg cagccagaca gagaggagct
     9661 ctcggctggt ccctgtgtca gctcccttcc ctcggtggac tctggcagca ctggtgcctt
     9721 ctcctcctct tctgcctcgc tattatccca ggactcgctc tcctcctcca ggtcggccag
     9781 cttctccatc tgcaccagtg tgtcctcggt aggggcgccc tccagcagct tctctgtcag
     9841 cctggagcca caggccttca gcagccaggc aaagctggtg tgcaggcagg cccacagatt
     9901 atcgtcggat gtcaggctgg tcagagagcg ggcggcattg ccgttcctct ggtgcagaaa
     9961 aggtgtgaag gcggtgttca tcctctgggc cctctgtgga cagccaaact gctcggcctc
    10021 gaacaccttc agggccttgc cctgcagctc gtcgatgatg cctctcacct tgcccagcag
    10081 aaagggccaa gagttgatca ggtagatccg gtccatcatg attgtgatga tggaatacca
    10141 ccgctggaag ccggcggcca ggctatcctt gataaagaag gtgtgagaaa acacgaagcc
    10201 gtgctgctcg tcgccaaaga agataggtcc ctctcttcct gggcacacct cgcaagacag
    10261 ggatctcacg caggcctgcc gcacgatgga gaacagctga gggtggctgg ggtgctggtg
    10321 agacacgtac ttgatgcttg tctccttatc gtgagagata tagccagggt gtcctgcggc
    10381 cagggaccga catccctcgc acatgtcgct cttctttggg ccaggagagg agctctccac
    10441 agaggctccc tctgctgggg agtgggctct catccggcta ttcatctgga tgccgccctc
    10501 ctcctcctct gcctgctctc cctgtcctgg gctatcctcg tttccgtcgc cctgtggcag
    10561 tggtgcgtgc agcacctctg tgcaaaacag ggtccgtggg ccgtgcagct cacagaagtg
    10621 gcacagggcc acgatggcgt tcatcatggt ggcaccggta gcgctagcgt ctaacaaaaa
    10681 agccaaaaac ggccagaatt tagcggaca
//
