LOCUS       sequence-328092-        5539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnt2a gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnt2a
FEATURES             Location/Qualifiers
     source          1..5539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(134..161)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(253..339)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     388..410
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     402..419
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     403..419
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    435..534
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3530..3629)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3646..3665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3647..3665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3670..3686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3670..3686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3788..3807)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3799..4608
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3866..3885)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4755..5343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5244..5263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5497..5514
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
      121 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
      181 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
      241 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
      301 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
      361 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
      421 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
      481 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca ggctccgcgg
      541 ccgccccctt caccatgaga atcctactga catatctacg atacaggggt aacatattgc
      601 agcaggtact tcaaatggca tttatcttgg aattgattga caccgtgcct tttgtaattg
      661 gaatttttta tcaatccttc cggaagctct tcgttcctgt ttttctgaac tgctggctgg
      721 caaaacgtgc tttggaaagc acggttaatg atcttcatcg agctattcac cggacacagt
      781 cagccatgtt caatcaggtc ctcatgctca tctccaccat tgtctgcctc attttcactt
      841 tcatttgtgg tgttgagcac ttgcagcgag ccggaacaaa actcacagtt tttgactctt
      901 tctacttctg catcgtgacc ttttctacag tggggtttgg agatgttgtt cctgatattt
      961 ggccatccaa gttgctggtg gttattatga tacttgtcac tttaatggtc ctgccaatcc
     1021 agtttgagga gttggcttat ctgtggatgg agcggcagaa gtctggagga tattacagca
     1081 gacagagagc agaaacagag aaacacgtgg tgttgtgtgt cagttcaatc aaaattgacc
     1141 tgctgatgga tttcctaaat gagttttatg cccaccctaa acttgaggag cactatgtca
     1201 tcatcctttg cccaactgag ctggatgcag cagtcagaag tgtgctgcgg atcccaatgt
     1261 ggtcacatag agtcatatat ctgcagggct ctgccctcaa agaccaagac ttggtcagag
     1321 caaaactgga ttatgctgag gcttgtttca ttctgacttg tcgctgtgtg gctgaccgca
     1381 atgcagcaga ttatcagacc atcctgcgtg cttgggcagt gaaggatttt gctccaggct
     1441 gtactctttt tgttcagatt ctcaaaccag aaaacaaatt tcatgtgaaa tttgcagatc
     1501 atattgtttg tgaggaagag tttaaatttg ccatgctggc catgaactgc atctgtcctg
     1561 caacatctac attcatcacc cttctggtgc acacctcaca aggcctagag ggccagcact
     1621 ctcctgagga ctggcacaga atttatggca aatgctcagg aaatgaagtg tacagtatag
     1681 ttctgaacaa aagcatcttc ttttctgagt atgaaggaaa caactttgct tatgcatcat
     1741 ttaatgcata taataagtat ggcatctgtc tcattggagt ctgtcctgag gacactaaga
     1801 gcattctgct gaatcctggt gctcagtata ccatgaatgc catggacacg tgcttctata
     1861 tcagtctcac caaggaggaa aactcctatg ttaagcagag agagtgtaga aacgacagca
     1921 agactcaacc gccaatgagg agtgccatta caagaccagg gaccatggcc atagatatac
     1981 aggaaataag cattccatcc actttaggcc catcgctctc aactggagct aatacatctg
     2041 agagaagaca accaatcatt attcctgttc tagaactgcc tgattcacct tcttttcagg
     2101 ctagcaagct actgagtggt cagtcaaaga gtcacataaa tggagaagaa agcaatgatg
     2161 gctactttat aggatatcct ccaaatttac cctacattgg aagctcgaca atcctttgcc
     2221 atttacgaaa aaagaaaatg cccacatgtt gtatgcatct ggaaaagttt ttgtccaaga
     2281 cctgcaacca caaagacgat gaagatgcaa aagtgtatag tttgaagagt aagctggtca
     2341 ttgtctctgt tgagacagca ggaaacagcc tgtacaactt catcctccca ttacgagcct
     2401 cgtacagacc acagaatgaa ctgtggccca ttgtgctgct cttggagcat gtaccggatg
     2461 aagaatttct tgagacgatc agttggtttc caatgattta ctacttgctt ggatcaatcg
     2521 acagcctgga tgacctgttg cgttgtggcg tttcttttgc tgccagcatg gtggttgtgg
     2581 ataaggaaag caccatgact gctaaggagg actacatggc ggatgcaaaa accatagtta
     2641 atgttcagac tctgttcagg ttgttctctg gtctgaacat catcactgag ttaactcact
     2701 ctgctaacat gagattcatg cagttcagca caaaggactg tcattccctg gcattctcca
     2761 aactagcaaa gaaagaaaga gaaaaaggct caaacctgtc cttcatattt caccttccgt
     2821 ttgctgctgg tagggttttc agcatcagca tgctagacac actcctctac cagtcttttg
     2881 ttaaagatta tatgataaca atcaccaggc tcctgctggg gttggaaact acaccacagt
     2941 ctggtttcct gtgtgctatg agcatcaccg aggaggacct atgcattgag acatatggca
     3001 ggctgtacca aaggctttcc tccactgtag gagatattcc catcggcatc tacaggacca
     3061 agtctcaaac agttgaatcc cctgagactc cacaattcca aatgtcagac aaggcatctg
     3121 tgtttgatga gggcggagac ctagatgagc aacatcagaa tcttcaatgc gaaataccag
     3181 acagtcaatt gcagacacag tctctgcgtc ggagaagcat gcagtgggct tccagtctcc
     3241 ttgggccatg gcacactgag cgtgaggcag acagattgag tcagcatcgg ggtgtcctgc
     3301 tttcatgctc tgagagactg gaggtcacag agctggtcaa gaaacgcatg aaaaacctgg
     3361 gcctatccac tctgggattt gatgaacaga ccaatatcaa gaacagccac gcttacatct
     3421 taatcaaccc atcttctgat acaaaactgg agctcaatga cattgtacat aatccgtaaa
     3481 gatactctat cttccaagct ggacaataac taaaagggtg ggcgcgccga cccagctttc
     3541 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     3601 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     3661 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     3721 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     3781 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     3841 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     3901 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     3961 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     4021 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     4081 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     4141 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     4201 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     4261 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     4321 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     4381 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     4441 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     4501 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     4561 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     4621 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     4681 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     4741 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4801 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4861 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4921 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4981 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5041 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5101 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5161 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5221 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5281 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5341 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5401 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     5461 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     5521 gaagagcgcc caatacgca
//
