LOCUS       sequence-328133-        4818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mCerulean3 fused to the N-terminus of the Bcl-2 family 
            protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to 
            make stable BMK-DKO cell lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Liu Q, Osterlund EJ, Chi X, Pogmore J, Leber B, Andrews DW
  TITLE     Bim escapes displacement by BH3-mimetic anti-cancer drugs by 
            double-bolt locking both Bcl-XL and Bcl-2.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 37689. doi: 10.7554/eLife.37689.
  PUBMED    30860026
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 37689. doi"; pages: " 
            10.7554/eLife.37689"
COMMENT     Sequence Label: mCerulean3-Bcl-2-s2193
FEATURES             Location/Qualifiers
     source          1..4818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    20..141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(31..50)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     85..104
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      329..916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     817..836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1038..1229
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1061..1081)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     regulatory      2351..2360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2357..2359,2360..2362,2363..3073)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2402..2423)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2663..2682)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3010..3031
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3830..4274
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        4308..4354
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4346..4364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctagctggc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
       61 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      121 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      181 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggaaatg
      241 ttaattaact agccatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
      301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg ggggttcgtg
      661 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      721 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      781 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      841 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      901 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      961 gccttttgct cacatgttct taattaacct gcagggcctg aaataacctc tgaaagagga
     1021 acttggttag gtaccttctg aggctgaaag aaccagctgt ggaatgtgtg tcagttaggg
     1081 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     1141 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     1201 catctcaatt agtcagcaac catagtccca ctagttccgc cagagcgcgc gagggcctcc
     1261 agcggccgcc cctcccccac agcaggggcg gggtcccgcg cccaccggaa ggagcgggct
     1321 cggggcgggc ggcgctgatt ggccggggcg ggcctgacgc cgacgcggct ataagagacc
     1381 acaagcgacc cgcagggcca gacgttcttc gccgaagctt gccgtcagaa cgcaggtgag
     1441 gggcgggtgt ggcttccgcg ggccgccgag ctggaggtcc tgctccgagc gggccgggcc
     1501 ccgctgtcgt cggcggggat tagctgcgag cattcccgct tcgagttgcg ggcggcgcgg
     1561 gaggcagagt gcgaggccta gcggcaaccc cgtagcctcg cctcgtgtcc ggcttgaggc
     1621 ctagcgtggt gtccgcgccg ccgccgcgtg ctactccggc cgcactctgg tctttttttt
     1681 ttttgttgtt gttgccctgc tgccttcgat tgccgttcag caataggggc taacaaaggg
     1741 agggtgcggg gcttgctcgc ccggagcccg gagaggtcat ggttggggag gaatggaggg
     1801 acaggagtgg cggctggggc ccgcccgcct tcggagcaca tgtccgacgc cacctggatg
     1861 gggcgaggcc tggggttttt cccgaagcaa ccaggctggg gttagcgtgc cgaggccatg
     1921 tggccccagc acccggcacg atctggcttg gcggcgccgc gttgccctgc ctccctaact
     1981 agggtgaggc catcccgtcc ggcaccagtt gcgtgcgtgg aaagatggcc gctcccgggc
     2041 cctgttgcaa ggagctcaaa atggaggacg cggcagcccg gtggagcggg cgggtgagtc
     2101 acccacacaa aggaagaggg cctggtccct caccggctgc tgcttcctgt gaccccgtgg
     2161 tcctatcggc cgcaatagtc acctcgggct tttgagcacg gctagtcgcg gcggggggag
     2221 gggatgtaat ggcgttggag tttgttcaca tttggtgggt ggagactagt caggccagcc
     2281 tggcgctgga agtcattttt ggaatttgtc cccttgagtt ttgagcggag ctaattcaaa
     2341 gcaaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2401 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2461 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2521 cgtgccctgg cccaccctcg tgaccaccct gagctggggc gtgcagtgct tcgcccgcta
     2581 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2641 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2701 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2761 caacatcctg gggcacaagc tggagtacaa cgccatccac ggcaacgtct atatcaccgc
     2821 cgacaagcag aagaacggca tcaaggccaa cttcggcctc aactgcaaca tcgaggacgg
     2881 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2941 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     3001 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3061 cgagctgtac aagtccggac tcagatccac gcgtatggcg cacgctggga gaacagggta
     3121 cgataaccgg gagatagtga tgaagtacat ccattataag ctgtcgcaga ggggctacga
     3181 gtgggatgcg ggagatgtgg gcgccgcgcc cccgggggcc gcccccgcac cgggcatctt
     3241 ctcctcccag cccgggcaca cgccccatcc agccgcatcc cgggacccgg tcgccaggac
     3301 ctcgccactg cagaccccgg ctgcccccgg cgccgccgcg gggcctgcgc tcagcccggt
     3361 gccacctgtg gtccacctga ccctccgcca ggccggcgac gacttctccc gccgctaccg
     3421 ccgcgacttc gccgagatgt ccagccagct gcacctgacg cccttcaccg cgcggggacg
     3481 ctttgccacg gtggtggagg agctcttcag ggacggggtg aactggggga ggattgtggc
     3541 cttctttgag ttcggtgggg tcatatgtgt ggagagcgtc aaccgggaga tgtcgcccct
     3601 ggtggacaac atcgccctgt ggatgactga gtacctgaac cggcacctgc acacctggat
     3661 ccaggataac ggaggctggg atgcctttgt ggagctctac ggccccagca tgcggcctct
     3721 gtttgatttc tcctggctgt ctcttaagac tctgctctcg ctagcactag tgggagcatg
     3781 catcaccctg ggtgcctatc tgtcccacaa gtgactcgtc gaccctagga gcaggtttcc
     3841 ccaatgacac aaaacgtgca acttgaaact ccgcctggtc tttccaggtc tagaggggta
     3901 acactttgta ctgcgtttgg ctccacgctc gatccactgg cgagtgttag taacagcact
     3961 gttgcttcgt agcggagcat gacggccgtg ggaactcctc cttggtaaca aggacccacg
     4021 gggccaaaag ccacgcccac acgggcccgt catgtgtgca accccagcac ggcgacttta
     4081 ctgcgaaacc cactttaaag tgacattgaa actggtaccc acacactggt gacaggctaa
     4141 ggatgccctt caggtacccc gaggtaacac gcgacactcg ggatctgaga aggggactgg
     4201 ggcttctata aaagcgctcg gtttaaaaag cttctatgcc tgaataggtg accggaggtc
     4261 ggcacctttc ctttgcaatt actgacccta tgaatacaac tgactgtttg acaattaatc
     4321 atcggcatag tatatcggca tagtataata cgactcacta taggagggcc accatgaaga
     4381 ccttcaacat ctctcagcag gatctggagc tggtggaggt cgccactgag aagatcacca
     4441 tgctctatga ggacaacaag caccatgtcg gggcggccat caggaccaag actggggaga
     4501 tcatctctgc tgtccacatt gaggcctaca ttggcagggt cactgtctgt gctgaagcca
     4561 ttgccattgg gtctgctgtg agcaacgggc agaaggactt tgacaccatt gtggctgtca
     4621 ggcaccccta ctctgatgag gtggacagat ccatcagggt ggtcagcccc tgtggcatgt
     4681 gcagagagct catctctgac tatgctcctg actgctttgt gctcattgag atgaatggca
     4741 agctggtcaa aaccaccatt gaggaactca tccccctcaa gtacaccagg aactaaacct
     4801 gaattcttcg aactcgag
//
