LOCUS       sequence-32814-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pLKO.1 Rheb1 shRNA #1
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    132..249
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        298..808
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     727..745
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(830..849)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ttggctttat atatcttgtg gaaggacgaa acaccggcct cagacatact ccatagatct
       61 cgagatctat ggagtatgtc tgaggttttt gaattctcga cctcgagaca aatggcagta
      121 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      181 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      241 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg
      301 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
      361 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
      421 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
      481 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
      541 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
      601 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
      661 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
      721 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
      781 tgaccgaatc accgacctct ctccccaggg ggatccaccg gagcttacca tgaccgagta
      841 caagcccacg tg
//
