LOCUS       sequence-32817-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Vilella-Bach M, Nuzzi P, Fang Y, Chen J
  TITLE     The FKBP12-rapamycin-binding domain is required for 
            FKBP12-rapamycin-associated protein kinase activity and G1 
            progression.
  JOURNAL   J Biol Chem. 1999 Feb 12. 274(7):4266-72.
  PUBMED    9933627
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Feb 12. 274(7):4266-72."
COMMENT     Sequence Label: pcDNA3-Flag mTOR S2035T
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
       61 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattctgc
      121 agatatccat cacactggcg gccgccatgg attataaaga tgatgatgat atgcttggaa
      181 ccggacctgc cgccgccacc accgctgcca ccacatctag caatgtgagc gtcctgcagc
      241 agtttgccag tggcctaaag agccggaatg aggaaaccag ggccaaagcc gccaaggagc
      301 tccagcacta tgtcaccatg gaactccgag agatgagtca agaggagtct actcgcttct
      361 atgaccaact gaaccatcac atttttgaat tggtttccag ctcagatgcc aatgagagga
      421 aaggtggcat cttggccata gctagcctca taggagtgga aggtgggaat gccacccgaa
      481 ttggcagatt tgccaactat cttcggaacc tcctcccctc caatgaccca gttgtcatgg
      541 aaatggcatc caaggccatt ggccgtcttg ccatggcagg ggacactttt taccgctgag
      601 tacgtggaat ttga
//
