LOCUS       sequence-32821-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Vilella-Bach M, Nuzzi P, Fang Y, Chen J
  TITLE     The FKBP12-rapamycin-binding domain is required for 
            FKBP12-rapamycin-associated protein kinase activity and G1 
            progression.
  JOURNAL   J Biol Chem. 1999 Feb 12. 274(7):4266-72.
  PUBMED    9933627
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Feb 12. 274(7):4266-72."
COMMENT     Sequence Label: pcDNA3-Flag mTOR S2035T D2357E
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
      121 ggaattctgc agatatccat cacactggcg gccgccatgg attataaaga tgatgatgat
      181 atgcttggaa ccggacctgc cgccgccacc accgctgcca ccacatctag caatgtgagc
      241 gtcctgcagc agtttgccag tggcctaaag agccggaatg aggaaaccag ggccaaagcc
      301 gccaaggagc tccagcacta tgtcaccatg gaactccgag agatgagtca agaggagtct
      361 actcgcttct atgaccaact gaaccatcac atttttgaat tggtttccag ctcagatgcc
      421 aatgagagga aaggtggcat cttggccata gctagcctca taggagtgga aggtgggaat
      481 gccacccgaa ttggcagatt tgccaactat cttcggaacc tcctcccctc caatgaccca
      541 gttgtcatgg aaatggcatc caaggccatt ggccgtcttg ccatggcagg ggacactttt
      601 accgctgagt acgtggaatt tgaggtgaag cgagccctgg aatggctggg tgctgaccgc
      661 aatgagggcc ggagacatgc agctgtcctg gttctccgtg agctggccat cagcgtccct
      721 accttcttct tccagcaagt gcaacccttc tttgacaaca tttttgtggc cgtgtgggac
      781 cccaaacagg ccatccgtga gggagctgta gccgcccttc gtgcctgtct gattctcaca
      841 acccagcgtg agccgaagga gatgcagaag cctcagtggt acaggcacac a
//
