LOCUS       sequence-328210-       12423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dTAG (degron tag) NLRP1 for ligand-controlled N-terminal 
            degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 12423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: dTAG NLRP1
FEATURES             Location/Qualifiers
     source          1..12423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..516
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     435..453
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(525..527,528..848)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             855..881
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             855..881
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             885..911
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    927..951
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      954..963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3267..3278
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5380..5404)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5456..6044
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5509..5529)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5927..5938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6116..6349
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6427..6548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6464..6483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6518..6537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6588..6723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6743..6762)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6744..6762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6772..6789)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6772..6788)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6781..6803)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6930..7385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7017..7036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7227..7248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7411..7515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7516..7584,7585..8376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7734..7753)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8547..9135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9036..9055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9289..9306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9423..9444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9459..9464,9465..9482,9483..9489)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9497..9513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9502..9524
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9521..9537
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9521..9537
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9556..9576
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9558..9576
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9604..9830
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9831..10011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10058..10183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10676..10909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11094..11138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11369..11579
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11391..11587
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11438..11573
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11619..12221
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11619..11638)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12115..12135
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12264..12381
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
       61 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
      121 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
      181 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
      241 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
      301 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
      361 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
      421 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
      481 tccctcgttg accgaatcac cgacctctct ccccaggggc tagcatggga gtgcaggtgg
      541 aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc
      601 actacaccgg gatgcttgaa gatggaaaga aagttgattc ctcccgggac agaaacaagc
      661 cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc
      721 agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca
      781 ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa
      841 aactggaagg cggctacccc tacgacgtgc ccgactacgc cggctatccg tatgatgtcc
      901 cggactatgc aggcatcgat agatcaacaa gtttgtacaa aaaagcaggc ttcgccacca
      961 tggctggcgg agcctggggc cgcctggcct gttacttgga gttcctgaag aaggaggagc
     1021 tgaaggagtt ccagcttctg ctcgccaata aagcgcactc caggagctct tcgggtgaga
     1081 cacccgctca gccagagaag acgagtggca tggaggtggc ctcgtacctg gtggctcagt
     1141 atggggagca gcgggcctgg gacctagccc tccatacctg ggagcagatg gggctgaggt
     1201 cactgtgcgc ccaagcccag gaaggggcag gccactctcc ctcattcccc tacagcccaa
     1261 gtgaacccca cctggggtct cccagccaac ccacctccac cgcagtgcta atgccctgga
     1321 tccatgaatt gccggcgggg tgcacccagg gctcagagag aagggttttg agacagctgc
     1381 ctgacacatc tggacgccgc tggagagaaa tctctgcctc actcctctac caagctcttc
     1441 caagctcccc agaccatgag tctccaagcc aggagtcacc caacgccccc acatccacag
     1501 cagtgctggg gagctgggga tccccacctc agcccagcct agcacccaga gagcaggagg
     1561 ctcctgggac ccaatggcct ctggatgaaa cgtcaggaat ttactacaca gaaatcagag
     1621 aaagagagag agagaaatca gagaaaggca ggcccccatg ggcagcggtg gtaggaacgc
     1681 ccccacaggc gcacaccagc ctacagcccc accaccaccc atgggagcct tctgtgagag
     1741 agagcctctg ttccacatgg ccctggaaaa atgaggattt taaccaaaaa ttcacacagc
     1801 tgctacttct acaaagacct caccccagaa gccaagatcc cctggtcaag agaagctggc
     1861 ctgattatgt ggaggagaat cgaggacatt taattgagat cagagactta tttggcccag
     1921 gcctggatac ccaagaacct cgcatagtca tactgcaggg ggctgctgga attgggaagt
     1981 caacactggc caggcaggtg aaggaagcct gggggagagg ccagctgtat ggggaccgct
     2041 tccagcatgt cttctacttc agctgcagag agctggccca gtccaaggtg gtgagtctcg
     2101 ctgagctcat cggaaaagat gggacagcca ctccggctcc cattagacag atcctgtcta
     2161 ggccagagcg gctgctcttc atcctcgatg gtgtagatga gccaggatgg gtcttgcagg
     2221 agccgagttc tgagctctgt ctgcactgga gccagccaca gccggcggat gcactgctgg
     2281 gcagtttgct ggggaaaact atacttcccg aggcatcctt cctgatcacg gctcggacca
     2341 cagctctgca gaacctcatt ccttctttgg agcaggcacg ttgggtagag gtcctggggt
     2401 tctctgagtc cagcaggaag gaatatttct acagatattt cacagatgaa aggcaagcaa
     2461 ttagagcctt taggttggtc aaatcaaaca aagagctctg ggccctgtgt cttgtgccct
     2521 gggtgtcctg gctggcctgc acttgcctga tgcagcagat gaagcggaag gaaaaactca
     2581 cactgacttc caagaccacc acaaccctct gtctacatta ccttgcccag gctctccaag
     2641 ctcagccatt gggaccccag ctcagagacc tctgctctct ggctgctgag ggcatctggc
     2701 aaaaaaagac ccttttcagt ccagatgacc tcaggaagca tgggttagat ggggccatca
     2761 tctccacctt cttgaagatg ggtattcttc aagagcaccc catccctctg agctacagct
     2821 tcattcacct ctgtttccaa gagttctttg cagcaatgtc ctatgtcttg gaggatgaga
     2881 aggggagagg taaacattct aattgcatca tagatttgga aaagacgcta gaagcatatg
     2941 gaatacatgg cctgtttggg gcatcaacca cacgtttcct attgggcctg ttaagtgatg
     3001 agggggagag agagatggag aacatctttc actgccggct gtctcagggg aggaacctga
     3061 tgcagtgggt cccgtccctg cagctgctgc tgcagccaca ctctctggag tccctccact
     3121 gcttgtacga gactcggaac aaaacgttcc tgacacaagt gatggcccat ttcgaagaaa
     3181 tgggcatgtg tgtagaaaca gacatggagc tcttagtgtg cactttctgc attaaattca
     3241 gccgccacgt gaagaagctt cagctgattg agggcaggca gcacagatca acatggagcc
     3301 ccaccatggt agtcctgttc aggtgggtcc cagtcacaga tgcctattgg cagattctct
     3361 tctccgtcct caaggtcacc agaaacctga aggagctgga cctaagtgga aactcgctga
     3421 gccactctgc agtgaagagt ctttgtaaga ccctgagacg ccctcgctgc ctcctggaga
     3481 ccctgcggtt ggctggctgt ggcctcacag ctgaggactg caaggacctt gcctttgggc
     3541 tgagagccaa ccagaccctg accgagctgg acctgagctt caatgtgctc acggatgctg
     3601 gagccaaaca cctttgccag agactgagac agccgagctg caagctacag cgactgcagc
     3661 tggtcagctg tggcctcacg tctgactgct gccaggacct ggcctctgtg cttagtgcca
     3721 gccccagcct gaaggagcta gacctgcagc agaacaacct ggatgacgtt ggcgtgcgac
     3781 tgctctgtga ggggctcagg catcctgcct gcaaactcat acgcctgggg ctggaccaga
     3841 caactctgag tgatgagatg aggcaggaac tgagggccct ggagcaggag aaacctcagc
     3901 tgctcatctt cagcagacgg aaaccaagtg tgatgacccc tactgagggc ctggatacgg
     3961 gagagatgag taatagcaca tcctcactca agcggcagag actcggatca gagagggcgg
     4021 cttcccatgt tgctcaggct aatctcaaac tcctggacgt gagcaagatc ttcccaattg
     4081 ctgagattgc agaggaaagc tccccagagg tagtaccggt ggaactcttg tgcgtgcctt
     4141 ctcctgcctc tcaaggggac ctgcatacga agcctttggg gactgacgat gacttctggg
     4201 gccccacggg gcctgtggct actgaggtag ttgacaaaga aaagaacttg taccgagttc
     4261 acttccctgt agctggctcc taccgctggc ccaacacggg tctctgcttt gtgatgagag
     4321 aagcggtgac cgttgagatt gaattctgtg tgtgggacca gttcctgggt gagatcaacc
     4381 cacagcacag ctggatggtg gcagggcctc tgctggacat caaggctgag cctggagctg
     4441 tggaagctgt gcacctccct cactttgtgg ctctccaagg gggccatgtg gacacatccc
     4501 tgttccaaat ggcccacttt aaagaggagg ggatgctcct ggagaagcca gccagggtgg
     4561 agctgcatca catagttctg gaaaacccca gcttctcccc cttgggagtc ctcctgaaaa
     4621 tgatccataa tgccctgcgc ttcattcccg tcacctctgt ggtgttgctt taccaccgcg
     4681 tccatcctga ggaagtcacc ttccacctct acctgatccc aagtgactgc tccattcgga
     4741 aggccataga tgatctagaa atgaaattcc agtttgtgcg aatccacaag ccacccccgc
     4801 tgaccccact ttatatgggc tgtcgttaca ctgtgtctgg gtctggttca gggatgctgg
     4861 aaatactccc caaggaactg gagctctgct atcgaagccc tggagaagac cagctgttct
     4921 cggagttcta cgttggccac ttgggatcag ggatcaggct gcaagtgaaa gacaagaaag
     4981 atgagactct ggtgtgggag gccttggtga aaccaggaga tctcatgcct gcaactactc
     5041 tgatccctcc agcccgcata gccgtacctt cacctctgga tgccccgcag ttgctgcact
     5101 ttgtggacca gtatcgagag cagctgatag cccgagtgac atcggtggag gttgtcttgg
     5161 acaaactgca tggacaggtg ctgagccagg agcagtacga gagggtgctg gctgagaaca
     5221 cgaggcccag ccagatgcgg aagctgttca gcttgagcca gtcctgggac cggaagtgca
     5281 aagatggact ctaccaagcc ctgaaggaga cccatcctca cctcattatg gaactctggg
     5341 agaagggcag caaaaaggga ctcctgccac tcagcagcga cccagctttc ttgtacaaag
     5401 tggttaccgg ttagtaatga actagttaag tcgacaatca acgcgttaag tcgacaatca
     5461 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5521 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5581 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5641 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5701 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5761 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5821 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5881 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5941 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6001 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     6061 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6121 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6181 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6241 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6301 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     6361 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6421 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6541 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6601 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6661 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6721 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6781 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6841 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6901 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     6961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7381 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7441 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7501 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7561 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7621 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7681 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7741 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7801 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7861 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7921 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7981 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8041 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8101 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8161 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8221 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8281 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8341 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8401 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8461 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9181 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9241 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9301 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9361 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     9421 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     9481 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     9541 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     9601 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     9661 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     9721 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     9781 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     9841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
    10021 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    10081 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    10141 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    10201 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    10261 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    10321 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    10381 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    10441 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    10501 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
    10561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    10621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
    10681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    10741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    10801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    10861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    10921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    10981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
    11041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    11101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    11161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    11221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
    11281 ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac
    11341 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa
    11401 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    11461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    11521 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    11581 ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg
    11641 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
    11701 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
    11761 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
    11821 acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
    11881 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
    11941 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
    12001 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
    12061 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
    12121 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
    12181 cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga
    12241 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
    12301 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    12361 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
    12421 cgg
//
