LOCUS       sequence-328214-        8393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of your protein of interest fused with TurboID and 
            mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search
            for client proteins within light-induced biomolecular condensates..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Frattini C, Promonet A, Alghoul E, Vidal-Eychenie S, Lamarque M, 
            Blanchard MP, Urbach S, Basbous J, Constantinou A
  TITLE     TopBP1 assembles nuclear condensates to switch on ATR signaling.
  JOURNAL   Mol Cell. 2021 Jan 16. pii: S1097-2765(20)30991-6. doi: 
            10.1016/j.molcel.2020.12.049.
  PUBMED    33503405
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 16. pii: S1097-2765(20)30991-6. doi: 
            10.1016/j.molcel.2020.12.049."
COMMENT     Sequence Label: pCDNA5_FRT_TO_TurboID-mCherry-Cry2
FEATURES             Location/Qualifiers
     source          1..8393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             753..1712
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2457..3950
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     complement(4111..4128)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4117..4341
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4474..4493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4625..4672
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4680..5702
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4680..5702
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5832..5953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5869..5888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5923..5942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6002..6018)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6002..6018)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6015..6037)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6026..6042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6050..6056,6057..6074,6075..6080))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6095..6116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6233..6250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6404..6992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6484..6503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7163..7954,7955..8023))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8024..8128)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8203..8222)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaaggcca ccatgaaaga caatactgtg cctctgaagc
      781 tgatcgctct cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg
      841 gcatgtccag ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg
      901 tgttcaccgt gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta
      961 aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca
     1021 ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag
     1081 aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta
     1141 acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc
     1201 cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag
     1261 tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc
     1321 tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta
     1381 tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga
     1441 ttaacctgga caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg
     1501 aactgttcga gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact
     1561 tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg
     1621 ggattgataa acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg
     1681 gcggagaaat cagtctgcgg tctgccgaaa agcacgtggg taccatcgat gctagcatgg
     1741 tgtctaaagg cgaggaggat aacatggcta taatcaagga atttatgagg ttcaaggtgc
     1801 atatggaggg atctgtgaac ggtcacgagt tcgaaatcga gggcgaaggc gaggggcgac
     1861 cctatgaagg gacacagacg gccaaactga aggtgaccaa gggtggcccc ctgcctttcg
     1921 cctgggacat cctgtcaccc cagtttatgt acggctctaa ggcttacgtt aagcaccctg
     1981 ccgatattcc ggactacttg aaactgagct tccctgaagg gttcaagtgg gaacgggtaa
     2041 tgaattttga ggatggcggt gtagtcacag ttacccagga cagctctctt caggacggag
     2101 aatttatcta taaggttaaa cttcggggca ctaacttccc atcagacggc cccgtgatgc
     2161 agaagaaaac tatgggctgg gaagccagct cagaacgcat gtatcccgag gacggagcac
     2221 tgaagggaga aatcaagcag cgactcaagc tgaaggatgg gggacattat gatgctgagg
     2281 tcaaaaccac ctataaggcc aagaaacccg ttcagcttcc tggggcatac aacgtgaata
     2341 tcaaactgga tattacatcc cataacgaag actataccat cgtggaacag tacgagcggg
     2401 ccgagggcag acatagcaca gggggcatgg atgaattgta caaggcggcc acgcgtatga
     2461 agatggacaa aaagactata gtttggttta gaagagacct aaggattgag gataatcctg
     2521 cattagcagc agctgctcac gaaggatctg tttttcctgt cttcatttgg tgtcctgaag
     2581 aagaaggaca gttttatcct ggaagagctt caagatggtg gatgaaacaa tcacttgctc
     2641 acttatctca atccttgaag gctcttggat ctgacctcac tttaatcaaa acccacaaca
     2701 cgatttcagc gatcttggat tgtatccgcg ttaccggtgc tacaaaagtc gtctttaacc
     2761 acctctatga tcctgtttcg ttagttcggg accataccgt aaaggagaag ctggtggaac
     2821 gtgggatctc tgtgcaaagc tacaatggag atctattgta tgaaccgtgg gagatatact
     2881 gcgaaaaggg caaacctttt acgagtttca attcttactg gaagaaatgc ttagatatgt
     2941 cgattgaatc cgttatgctt cctcctcctt ggcggttgat gccaataact gcagcggctg
     3001 aagcgatttg ggcgtgttcg attgaagaac tagggctgga gaatgaggcc gagaaaccga
     3061 gcaatgcgtt gttaactaga gcttggtctc caggatggag caatgctgat aagttactaa
     3121 atgagttcat cgagaagcag ttgatagatt atgcaaagaa cagcaagaaa gttgttggga
     3181 attctacttc actactttct ccgtatctcc atttcgggga aataagcgtc agacacgttt
     3241 tccagtgtgc ccggatgaaa caaattatat gggcaagaga taagaacagt gaaggagaag
     3301 aaagtgcaga tctttttctt aggggaatcg gtttaagaga gtattctcgg tatatatgtt
     3361 tcaacttccc gtttactcac gagcaatcgt tgttgagtca tcttcggttt ttcccttggg
     3421 atgctgatgt tgataagttc aaggcctgga gacaaggcag gaccggttat ccgttggtgg
     3481 atgccggaat gagagagctt tgggctaccg gatggatgca taacagaata agagtgattg
     3541 tttcaagctt tgctgtgaag tttcttctcc ttccatggaa atggggaatg aagtatttct
     3601 gggatacact tttggatgct gatttggaat gtgacatcct tggctggcag tatatctctg
     3661 ggagtatccc cgatggccac gagcttgatc gcttggacaa tcccgcgtta caaggcgcca
     3721 aatatgaccc agaaggtgag tacataaggc aatggcttcc cgagcttgcg agattgccaa
     3781 ctgaatggat ccatcatcca tgggacgctc ctttaaccgt actcaaagct tctggtgtgg
     3841 aactcggaac aaactatgcg aaacccattg tagacatcga cacagctcgt gagctactag
     3901 ctaaagctat ttcaagaacc cgtgaagcac agatcatgat cggagcagca gcccgggatc
     3961 caccggtcgc caccggttca gggagcggat ccggctcagg gagtgcggcc gcaactccca
     4021 cctgcaacat gcgtgactga ggcgcgcctg tacagatatc tgcggccgcc taactcgagt
     4081 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     4141 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4201 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4261 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4321 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4381 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4441 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4501 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4561 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4621 cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact tccttggcca
     4681 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     4741 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     4801 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     4861 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     4921 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     4981 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     5041 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     5101 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     5161 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     5221 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     5281 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     5341 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     5401 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     5461 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     5521 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     5581 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     5641 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     5701 agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5761 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5821 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5881 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5941 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     6001 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6061 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6121 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6181 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6241 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6301 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6361 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6841 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6961 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7021 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7081 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7141 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7201 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7261 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7321 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7381 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7441 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7501 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7561 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7621 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7681 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7741 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7801 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7861 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7921 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7981 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8041 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8101 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8161 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     8221 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     8281 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     8341 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
