LOCUS       sequence-328216-        3438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway pDONR vector containing NLRP1 FIIND (no stop codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pDONR221 NLRP1 FIIND (F1079M-L1373)
FEATURES             Location/Qualifiers
     source          1..3438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1563..1662)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1679..1698)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1680..1698)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1703..1719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1703..1719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1821..1840)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1832..2641
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1899..1918)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2788..3376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3277..3296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gccaccatgt ggggccccac ggggcctgtg gctactgagg tagttgacaa
      721 agaaaagaac ttgtaccgag ttcacttccc tgtagctggc tcctaccgct ggcccaacac
      781 gggtctctgc tttgtgatga gagaagcggt gaccgttgag attgaattct gtgtgtggga
      841 ccagttcctg ggtgagatca acccacagca cagctggatg gtggcagggc ctctgctgga
      901 catcaaggct gagcctggag ctgtggaagc tgtgcacctc cctcactttg tggctctcca
      961 agggggccat gtggacacat ccctgttcca aatggcccac tttaaagagg aggggatgct
     1021 cctggagaag ccagccaggg tggagctgca tcacatagtt ctggaaaacc ccagcttctc
     1081 ccccttggga gtcctcctga aaatgatcca taatgccctg cgcttcattc ccgtcacctc
     1141 tgtggtgttg ctttaccacc gcgtccatcc tgaggaagtc accttccacc tctacctgat
     1201 cccaagtgac tgctccattc ggaaggccat agatgatcta gaaatgaaat tccagtttgt
     1261 gcgaatccac aagccacccc cgctgacccc actttatatg ggctgtcgtt acactgtgtc
     1321 tgggtctggt tcagggatgc tggaaatact ccccaaggaa ctggagctct gctatcgaag
     1381 ccctggagaa gaccagctgt tctcggagtt ctacgttggc cacttgggat cagggatcag
     1441 gctgcaagtg aaagacaaga aagatgagac tctggtgtgg gaggccttgg tgaaaccagg
     1501 agatctcatg cctgcaacta ctctgatccc tccagcccgc atagccgtac cttcacctct
     1561 ggacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1621 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1681 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1741 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     1801 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     1861 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     1921 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     1981 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2041 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2101 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2161 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2221 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2281 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2341 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2401 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     2461 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     2521 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     2581 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     2641 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     2701 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     2761 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2821 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2881 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2941 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3001 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3061 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3121 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3181 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3241 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3301 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3361 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3421 gccttttgct cacatgtt
//
