LOCUS       sequence-328217-        5229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR gateway shuttling vector with DPP9L (no stop codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pDONR221 DPP9L
FEATURES             Location/Qualifiers
     source          1..5229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3354..3453)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3470..3489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3471..3489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3494..3510)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3494..3510)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3612..3631)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3623..4432
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3690..3709)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4579..5167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5068..5087
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gccaccatgc ggaaggttaa gaaactgcgc ctggacaagg agaacaccgg
      721 aagttggaga agcttctcgc tgaattccga gggggctgag aggatggcca ccaccgggac
      781 cccaacggcc gaccgaggcg acgcagccgc cacagatgac ccggccgccc gcttccaggt
      841 gcagaagcac tcgtgggacg ggctccggag catcatccac ggcagccgca agtactcggg
      901 cctcattgtc aacaaggcgc cccacgactt ccagtttgtg cagaagacgg atgagtctgg
      961 gccccactcc caccgcctct actacctggg aatgccatat ggcagccgag agaactccct
     1021 cctctactct gagattccca agaaggtccg gaaagaggct ctgctgctcc tgtcctggaa
     1081 gcagatgctg gatcatttcc aggccacgcc ccaccatggg gtctactctc gggaggagga
     1141 gctgctgagg gagcggaaac gcctgggggt cttcggcatc acctcctacg acttccacag
     1201 cgagagtggc ctcttcctct tccaggccag caacagcctc ttccactgcc gcgacggcgg
     1261 caagaacggc ttcatggtgt cccctatgaa accgctggaa atcaagaccc agtgctcagg
     1321 gccccggatg gaccccaaaa tctgccctgc cgaccctgcc ttcttctcct tcatcaataa
     1381 cagcgacctg tgggtggcca acatcgagac aggcgaagag cggcggctga ccttctgcca
     1441 ccaaggttta tccaatgtcc tggatgaccc caagtctgcg ggtgtggcca ccttcgtcat
     1501 acaggaagag ttcgaccgct tcactgggta ctggtggtgc cccacagcct cctgggaagg
     1561 ttcagagggc ctcaagacgc tgcgaatcct gtatgaggaa gtcgatgagt ccgaggtgga
     1621 ggtcattcac gtcccctctc ctgcgctaga agaaaggaag acggactcgt atcggtaccc
     1681 caggacaggc agcaagaatc ccaagattgc cttgaaactg gctgagttcc agactgacag
     1741 ccagggcaag atcgtctcga cccaggagaa ggagctggtg cagcccttca gctcgctgtt
     1801 cccgaaggtg gagtacatcg ccagggccgg gtggacccgg gatggcaaat acgcctgggc
     1861 catgttcctg gaccggcccc agcagtggct ccagctcgtc ctcctccccc cggccctgtt
     1921 catcccgagc acagagaatg aggagcagcg gctagcctct gccagagctg tccccaggaa
     1981 tgtccagccg tatgtggtgt acgaggaggt caccaacgtc tggatcaatg ttcatgacat
     2041 cttctatccc ttcccccaat cagagggaga ggacgagctc tgctttctcc gcgccaatga
     2101 atgcaagacc ggcttctgcc atttgtacaa agtcaccgcc gttttaaaat cccagggcta
     2161 cgattggagt gagcccttca gccccgggga agatgaattt aagtgcccca ttaaggaaga
     2221 gattgctctg accagcggtg aatgggaggt tttggcgagg cacggctcca agatctgggt
     2281 caatgaggag accaagctgg tgtacttcca gggcaccaag gacacgccgc tggagcacca
     2341 cctctacgtg gtcagctatg aggcggccgg cgagatcgta cgcctcacca cgcccggctt
     2401 ctcccatagc tgctccatga gccagaactt cgacatgttc gtcagccact acagcagcgt
     2461 gagcacgccg ccctgcgtgc acgtctacaa gctgagcggc cccgacgacg accccctgca
     2521 caagcagccc cgcttctggg ctagcatgat ggaggcagcc agctgccccc cggattatgt
     2581 tcctccagag atcttccatt tccacacgcg ctcggatgtg cggctctacg gcatgatcta
     2641 caagccccac gccttgcagc cagggaagaa gcaccccacc gtcctctttg tatatggagg
     2701 cccccaggtg cagctggtga ataactcctt caaaggcatc aagtacttgc ggctcaacac
     2761 actggcctcc ctgggctacg ccgtggttgt gattgacggc aggggctcct gtcagcgagg
     2821 gcttcggttc gaaggggccc tgaaaaacca aatgggccag gtggagatcg aggaccaggt
     2881 ggagggcctg cagttcgtgg ccgagaagta tggcttcatc gacctgagcc gagttgccat
     2941 ccatggctgg tcctacgggg gcttcctctc gctcatgggg ctaatccaca agccccaggt
     3001 gttcaaggtg gccatcgcgg gtgccccggt caccgtctgg atggcctacg acacagggta
     3061 cactgagcgc tacatggacg tccctgagaa caaccagcac ggctatgagg cgggttccgt
     3121 ggccctgcac gtggagaagc tgcccaatga gcccaaccgc ttgcttatcc tccacggctt
     3181 cctggacgaa aacgtgcact ttttccacac aaacttcctc gtctcccaac tgatccgagc
     3241 agggaaacct taccagctcc agatctaccc caacgagaga cacagtattc gctgccccga
     3301 gtcgggcgag cactatgaag tcacgttgct gcactttcta caggaatacc tcgacccagc
     3361 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     3421 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     3481 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     3541 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     3601 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     3661 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     3721 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     3781 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     3841 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     3901 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     3961 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     4021 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     4081 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     4141 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     4201 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     4261 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     4321 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     4381 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     4441 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
     4501 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     4561 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4621 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4681 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4741 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4801 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4861 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4921 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4981 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5041 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5101 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5161 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5221 tcacatgtt
//
