LOCUS       sequence-328218-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with 
            flanking Nextera sequencing adapters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Pierce SE, Granja JM, Greenleaf WJ
  TITLE     High-throughput single-cell chromatin accessibility CRISPR screens 
            enable unbiased identification of regulatory networks in cancer.
  JOURNAL   Nat Commun. 2021 May 20;12(1):2969. doi: 10.1038/s41467-021-23213-w.
  PUBMED    34016988
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-20"; volume: "12(1):2969. doi"; pages: " 
            10.1038/s41467-021-23213-w"
COMMENT     Sequence Label: pSP618
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    154..271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(569..613)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    798..1031
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1528..1653
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1700..1880
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1898..2096)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1923..1943)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2098..2477
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2649..2668
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2743..2847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2848..2916,2917..3708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3066..3085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3879..4467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4368..4387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4529..4709
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     5218..5234
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5219..5235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5238..5826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5344..5355
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5753..5773
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    5882..5915
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6185..6203
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     6207..6227
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    6435..6453
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     promoter        6466..7644
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6697..7635
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7592..7612
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7656..8252
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7656..7675)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8152..8172
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             8262..8315
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8328..9026
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     primer_bind     complement(9070..9087)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    join(9076..9205,1..95)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
       61 tagcaggcat gctggggatg cggtgggctc tatggtctag agcgggcccg gtactaacca
      121 aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt
      181 ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact
      241 gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtctgca
      301 gaattggcgc acgcagtgcc gatccgttca ctaatcgaat ggatctgtct ctgtctctct
      361 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
      421 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
      481 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
      541 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
      601 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
      661 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
      721 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
      781 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
      841 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
      901 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
      961 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     1021 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     1081 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     1141 aattgtccct catatctcct cctccaggtc tgaagatcag cggccggccg cttgctgtgc
     1201 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     1261 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     1321 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     1381 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
     1441 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     1501 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     1561 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     1621 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     1681 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     1741 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     1801 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     1861 tggtctaacc agagagaccc agtacaggca aaacgcgctg cttatataga cctcccaccg
     1921 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     1981 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     2041 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     2101 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     2161 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     2221 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     2281 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     2341 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     2401 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     2461 agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg
     2521 cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta
     2581 aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct
     2641 taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat cgggagatct
     2701 cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2821 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2881 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2941 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3001 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3061 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3121 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3181 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3241 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3301 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3361 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3421 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3481 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3541 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3601 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3661 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3721 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3781 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4021 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4501 gccttttgct ggccttttgc tcacatgctg ctagagattt tccacactga ctaaaagggt
     4561 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     4621 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     4681 gctcagatct ggtctaacca gagagaccca gtacagtccg gatgcagctc tcgggccatg
     4741 tgatgaaatg ctaggcggct gtcaaacctc cactctaata cttctctctc cgggtcatcc
     4801 atcccatgca ggctcacagg gtgtaacaag cgggtgttct ctccttcatt ggcttcttct
     4861 accttctctt gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg
     4921 atccctggcc ctggtgtgta gttctgccaa tcagggaagt agccttgtgt gtggtagatc
     4981 cacagatcaa ggatatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
     5041 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc
     5101 tgaggtgtga ctggaaaacc cacctcctcc tcctcttgtg cttctagcca ggcacaatca
     5161 gcattggtag ctgctgtatt gctacttgtg attgctccat gtttttctag gtctcgatcg
     5221 aggtcgacgg tatcgatgcg gggaggcggc ccaaagggag atccgactcg tctgagggcg
     5281 aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc
     5341 tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac
     5401 acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt
     5461 gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt
     5521 ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc
     5581 ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac
     5641 gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa
     5701 agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt
     5761 aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt aatccagagg
     5821 ttgattatcg ataagcttga tatcgaattg tacctagtgg aaccggaacc cttaaacatg
     5881 tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgacgt taacccagtc
     5941 tgactatcac gatggtaata cggttatcca cgcgggatcc gacgcgccat ctctaggccc
     6001 gcgccggccc cctcgcacgg acttgtggga gaagctcggc tactcccctg ccccggttaa
     6061 tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat
     6121 ctgttctttt taatactagc tacattttac atgataggct tggatttctg tctcgtgggc
     6181 tcggagatgt gtataagaga cagaaacagc acaaaaggaa actcacccta actgtaaagt
     6241 aattgtgtgt tttgagacta taagtatccc ttggagaacc accttgttgg cgaacttaat
     6301 cccgtggcaa gtttaagagc taagctggaa acagcatagc aagtttaaat aaggctagtc
     6361 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tgctcgaatc tacactcagc
     6421 ctcggaatca gtatctgtct cttatacaca tctgacgctg ccgacgatgc atgtgcccgt
     6481 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     6541 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     6601 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     6661 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     6721 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     6781 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     6841 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     6901 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     6961 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     7021 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     7081 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     7141 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     7201 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     7261 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     7321 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     7381 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgcgcttt
     7441 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     7501 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     7561 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     7621 tttcttccat ttcaggtgtc gtgagctagc ccaccatgac cgagtacaag cccacggtgc
     7681 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     7741 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     7801 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7861 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7921 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7981 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     8041 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     8101 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     8161 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     8221 gcacctggtg catgacccgc aagcccggtg ccggatcggg agagggcaga ggaagtctgc
     8281 taacatgcgg tgacgtcgag gagaatcctg gcccaccggt cgccaccagc gagctgatta
     8341 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
     8401 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     8461 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
     8521 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
     8581 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     8641 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     8701 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
     8761 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
     8821 gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga
     8881 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
     8941 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta
     9001 gcaaactggg gcacaagctt aattaagaat tcctagggcc tagagggccc gtttaaaccc
     9061 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     9121 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     9181 ttgcatcgca ttgtctgagt aggtg
//
