LOCUS       sequence-32823-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Vilella-Bach M, Nuzzi P, Fang Y, Chen J
  TITLE     The FKBP12-rapamycin-binding domain is required for 
            FKBP12-rapamycin-associated protein kinase activity and G1 
            progression.
  JOURNAL   J Biol Chem. 1999 Feb 12. 274(7):4266-72.
  PUBMED    9933627
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Feb 12. 274(7):4266-72."
COMMENT     Sequence Label: pcDNA3-Flag mTOR S2035T R2109A
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagcttgggg ggcggccgcc atggattata aagatgatga tgatatgctt
      121 ggaaccggac ctgccgccgc caccaccgct gccaccacat ctagcaatgt gagcgtcctg
      181 cagcagtttg ccagtggcct aaagagccgg aatgaggaaa ccagggccaa agccgccaag
      241 gagctccagc actatgtcac catggaactc cgagagatga gtcaagagga gtctactcgc
      301 ttctatgacc aactgaacca tcacattttt gaattggttt ccagctcaga tgccaatgag
      361 aggaaaggtg gcatcttggc catagctagc ctcataggag tggaaggtgg gaatgccacc
      421 cgaattggca gatttgccaa ctatcttcgg aacctcctcc cctccaatga cccagttgtc
      481 atggaaatgg catccaaggc cattggccgt cttgccatgg caggggacac ttttaccgct
      541 gagtacgtgg aatttgaggt gaagcgagcc ctggaatggc tgggtgctga ccgcaatgag
      601 ggccggagac atgcagctgt cctggttctc cgtgagctgg ccatcagcgt ccctaccttc
      661 ttcttccagc aagtgcaacc cttctttgac acatttttgt ggccgtgtgg gaccccaaac
      721 aggccatccg tgagggagct gtagccgccc ttcgtgcctg tctgattctc ac
//
