LOCUS       sequence-328231-         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-99a-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-99a-5p
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(106..130)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(354..373)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    419..474
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(473..492)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aggaggccga caaagagaca tacgtcgagc agcacgaggt ggctgtggcc agatactgcg
       61 acctccctag caaactgggg cacaaactta attgagaatt ctaccaccca gctttcttgt
      121 acaaagtggt acgtctcaag accagaggtc tctgcttcgc ttagtaacaa tacaacttac
      181 tacctcaaac aatacaactt actacctcaa acaatacaac ttactacctc aaacaataca
      241 acttactacc tcacaaccag aagagacctg tgcttcactc ctcaggtgca ggctgcctat
      301 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
      361 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
      421 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
      481 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
      541 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
      601 tatatgaaac agccccctgc tgtcca
//
