LOCUS       sequence-328232-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-99a-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-99a-3p
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..131)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(355..374)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    420..475
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(474..493)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aaggaggccg acaaagagac atacgtcgag cagcacgagg tggctgtggc cagatactgc
       61 gacctcccta gcaaactggg gcacaaactt aattgagaat tctaccaccc agctttcttg
      121 tacaaagtgg tacgtctcaa gaccagaggt ctctgcttgt tcggcccaga cccatagaag
      181 cgagcttgca gacccataga agcgagcttg cagacccata gaagcgagct tgcagaccca
      241 tagaagcgag cttgcaacca gaagagacct gtgcttcact cctcaggtgc aggctgccta
      301 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
      361 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
      421 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
      481 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
      541 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
      601 gtatatgaaa cagccccctg ctgtccattc c
//
