LOCUS       sequence-32826-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Vilella-Bach M, Nuzzi P, Fang Y, Chen J
  TITLE     The FKBP12-rapamycin-binding domain is required for 
            FKBP12-rapamycin-associated protein kinase activity and G1 
            progression.
  JOURNAL   J Biol Chem. 1999 Feb 12. 274(7):4266-72.
  PUBMED    9933627
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Feb 12. 274(7):4266-72."
COMMENT     Sequence Label: pGEX-2T FRB R2109A
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..306
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFARISK"
     primer_bind     complement(370..392)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(537..555)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        623..727
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtggatccg agctgatccg agtggccatc ctctggcatg agatgtggca tgaaggcctg
       61 gaagaggcat ctcgtttgta ctttggggaa aggaacgtga aaggcatgtt tgaggtgctg
      121 gagcccttgc atgctatgat ggaacggggc ccccagactc tgaaggaaac atcctttaat
      181 caggcctatg gtcgagattt aatggaggcc caagagtggt gcaggaagta catgaaatca
      241 gggaatgtca aggacctcac ccaagcctgg gacctctatt atcatgtgtt cgcacgaatc
      301 tcaaagcagt agaattcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga
      361 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
      421 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
      481 agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct
      541 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      601 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      661 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      721 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      781 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      841 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagag
//
