LOCUS       sequence-32827-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Fang Y, Vilella-Bach M, Bachmann R, Flanigan A, Chen J
  TITLE     Phosphatidic acid-mediated mitogenic activation of mTOR signaling.
  JOURNAL   Science. 2001 Nov 30. 294(5548):1942-5.
  PUBMED    11729323
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2001 Nov 30. 294(5548):1942-5."
COMMENT     Sequence Label: pcDNA-Myc S6K1
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             87..116
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttatggaac aaaaactcat ctcagaagag gatctgagcg
      121 gccgcatggc aggagtgttt gacatagacc tggaccagcc agaggatgca ggctctgagg
      181 atgagctgga ggaggggggt cagttaaatg aaagcatgga ccatggggga gttggaccat
      241 atgaacttgg catggaacat tgtgagaaat ttgaaatctc agaaactagt gtgaacagag
      301 ggccagaaaa aatcagacca gaatgttttg agctacttcg ggtacttggt aaagggggct
      361 atggaaaggt ttttcaagta cgaaaagtaa caggagcaaa tactgggaag atatttgcca
      421 tgaaggtgct taaaaaggca atgatagtaa gaaatgctaa agatacagct catacaaaag
      481 cagagcggaa tattctggag gaagtaaagc atcccttcat cgtggattta atttatgcct
      541 ttcagaccgg tggaaaactc tacctcatcc ttgagtatct cagtggagga gaactattta
      601 tgcagttaga aagagagggg atattcatgg aagatacagc ttgcttttac ttggctgaaa
      661 tctccatggc tttggggcat ttacatcaaa aagggatcat ctacagagac ctgaagccgg
      721 agaacatcat gcttaatcac caaggtcacg tgaagctgac agactttgga ctatgcaaag
      781 aatctattca tgatggaaca gtcacgcaca cattttgtgg aacaatagaa tacatggccc
      841 ctgaaatctt gatgagaagc ggccacaacc gtgctgtgga ttggtggagt ttgggagcat
      901 taatgtatga catgctgact ggagcacctc cattc
//
