LOCUS       sequence-328274-       11830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro
            from the EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, 
            Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH,
            Walensky LD, Marto JA, Dhe-Paganon S, Segal RA
  TITLE     Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors 
            promotes axon survival.
  JOURNAL   J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 
            10.1083/jcb.202005051.
  PUBMED    33284322
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-01-4"; volume: "220(1). pii: 211577. doi"; 
            pages: " 10.1083/jcb.202005051"
COMMENT     Sequence Label: pLV-EF1a-KIF5A-HA-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3386..3395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6482..6508
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6624..7173
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6768..6785)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6995..7014
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7176..7775
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7176..7195)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7672..7692
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7799..8387
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7852..7872)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8270..8281)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8594..9227
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9355..9371)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9355..9371)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9368..9390)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9379..9395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9403..9409,9410..9427,9428..9433))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9448..9469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9586..9603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9757..10345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9837..9856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10516..11307,11308..11376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11139..11158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11377..11481)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11529..11663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11566..11585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11620..11639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatcgccac catggcggag actaacaacg aatgcagcat
     3421 caaggtgctt tgccgatttc ggcccctgaa ccaggccgag attctgcggg gggacaagtt
     3481 catccccatt ttccaagggg acgacagcgt cattattggg ggaaagccat atgtctttga
     3541 ccgcgtcttc cccccaaaca ccactcagga gcaggtttac cacgcctgtg ccatgcagat
     3601 cgtcaaagac gtccttgctg gttacaatgg cacaatcttc gcttatggac agacatcctc
     3661 agggaaaacg cataccatgg aggggaagct gcacgaccct cagctgatgg gcatcattcc
     3721 ccggatcgct cgagacatct tcaaccacat ctactccatg gatgagaacc ttgaattcca
     3781 cattaaggta tcttacttcg agatttacct ggataagatc cgtgaccttt tggatgtgac
     3841 caagacgaac ctgtccgtgc atgaggacaa aaaccgggtg ccgtttgtca agggttgtac
     3901 cgaacgcttt gtgtccagcc cagaggagat tctggatgtg atcgatgagg ggaagtccaa
     3961 ccgtcacgta gctgtcacca acatgaacga gcacagttct cggagccaca gcatcttcct
     4021 catcaacatc aagcaggaga acgtagagac cgagcagaag ctcagcggga agctgtacct
     4081 cgtggatctg gccggaagcg agaaggtcag caagacaggg gcagagggag ccgttctgga
     4141 cgaggcaaag aatatcaaca agtcgctgtc ggccctgggg aacgtgatct ctgcactggc
     4201 agagggcacc aaaagctatg tgccgtaccg cgacagcaaa atgacgagga ttctccagga
     4261 ctctctggaa gggaactgca ggactaccat gttcatctgc tgctcgccgt ccagctacaa
     4321 tgacgcagag accaagtcca cgctcatgtt tgggcagcgg gcgaagacca tcaagaacac
     4381 tgcctcagtg aatctggagc tgactgctga gcagtggaag aagaagtatg agaaggagaa
     4441 ggagaagacc aagacccaga aggagacaat tgcgaagcta gaggctgagc ttagccggtg
     4501 gcgcaatgga gagaatgtgc ctgagactga gcgcctggct ggagaggact cagctctggg
     4561 agctgagctc tgcgaggaga cccctgtgaa tgacaactca tccattgtgg tacgcatcgc
     4621 acctgaggaa aggcagaaat atgaggaaga gatccgccgt ctctacaagc agcttgatga
     4681 caaggatgat gagatcaacc agcagagcca gctcattgag aagctgaagc agcagatgct
     4741 ggaccaggaa gagctgctcg tgtccactcg gggagacaac gagaaggtcc agcgggagct
     4801 tagccacctg cagtccgaga acgatgctgc gaaggacgag gtgaaggaag tgctgcaggc
     4861 cctagaggag ctggcggtca actacgacca gaagtcccag gaggtggagg agaagagcca
     4921 gcagaaccag ctgctggtgg acgagctgtc ccagaaagtg gccaccatgc tgtccctgga
     4981 gtccgagcta cagcggctcc aggaggtcag tggacaccag cgaaagcgga tcgctgaggt
     5041 gctgaatggg ctgatgaggg acctgagtga gttcagtgtc atcgtgggca acggcgagat
     5101 taagctgccc gtggagatca gtggggccat cgaggaggag ttcacggtgg cccggctcta
     5161 catcagcaag atcaagtcgg aggtgaagtc cgtggttaag cgatgtcggc agctggagaa
     5221 cctccaggtg gagtgtcatc gcaagatgga ggtgaccggt agggagctgt catcttgcca
     5281 actgctcatc tcacagcatg aggccaagat ccgttcactc acggagtaca tgcagactgt
     5341 ggagttgaag aaacggcacc tggaagagtc ctacgactcc ctgagcgatg agcttgccag
     5401 gctccaggcg cacgaaactg tacacgaggt agctctgaaa gacaaggagc cagacacaca
     5461 ggacgcggag gaggtgaaga aggccctgga actacagatg gagaatcacc gtgaggccca
     5521 tcaccggcag ctggcccgcc tccgggatga gattaatgag aaacagaaaa ccattgatga
     5581 gctgaaagac ctgaaccaga agctccagtt agagctggag aagcttcagg ccgactatga
     5641 gaggctgaag aatgaagaga atgagaagag cgccaagctc caggagctga catttctgta
     5701 tgagcgacat gagcagtcca agcaggacct caaggggctg gaggagacag ttgcccgtga
     5761 actccagacc ctccacaacc ttcgcaagct gttcgttcaa gacgtcacga ctcgagtcaa
     5821 gaaaagtgca gaaatggagc ccgaggacag tggggggatt cattcccaaa agcagaagat
     5881 ctcctttctt gagaacaacc tggaacagct tacaaaggtt cacaaacagc tggtacgtga
     5941 caatgcagat ctgcgttgtg agcttcctaa attggaaaaa cgacttcggg ctacggctga
     6001 gagagttaag gccctggagg gtgcactgaa ggaggccaag gagggtgcta tgaaggacaa
     6061 gcgtagatac cagcaggagg tggaccgcat caaggaagcc gtgcggtaca agagctccgg
     6121 caagcggggc cattctgccc agatcgctaa gcctgtgagg cctggccact atcctgcctc
     6181 ctcacccacc aacccctacg gcacccggag ccccgagtgt atcagctaca ccaacaacct
     6241 cctccagaac taccagaacc tgcacctgca ggctgcgcct agctccactt cagatatgta
     6301 ctttgccagt agcggagcca catctgttgc ccccttggct tcctaccaga aggccaacat
     6361 ggacaatgga aatgccacag atatcaacga caacaggagt gacctgccgt gtggctatga
     6421 ggctgaggac caggccaagc ttttccctct ccaccaagag acagcagcca gtggatccgg
     6481 atacccatac gatgttccag attacgctta aaattcctcg agggcggccg ctctagagtc
     6541 gacgggccgc ggtaacaatt gttaactaac ttaagctagc aacggtttcc ctctagcggg
     6601 atcaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     6661 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     6721 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     6781 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     6841 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     6901 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     6961 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     7021 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     7081 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     7141 ggacgtggtt ttcctttgaa aaacacgata ataccatgac cgagtacaag cccacggtgc
     7201 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7261 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     7321 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7381 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7441 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7501 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7561 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7621 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     7681 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     7741 gcacctggtg catgacccgc aagcccggtg cctgacccgg gcggggcgcg tctggaacaa
     7801 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7861 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7921 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7981 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     8041 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     8101 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8161 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     8221 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8281 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8341 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     8401 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     8461 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     8521 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8581 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     8641 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     8701 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     8761 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     8821 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     8881 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     8941 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     9001 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     9061 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9121 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9181 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     9241 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     9301 ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9361 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9421 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9481 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    11521 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    11581 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    11641 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    11701 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    11761 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    11821 acttagtagt
//
