LOCUS       sequence-32828-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Kim JE, Chen J
  TITLE     Cytoplasmic-nuclear shuttling of FKBP12-rapamycin-associated protein
            is involved in rapamycin-sensitive signaling and translation 
            initiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Dec 19. 97(26):14340-5.
  PUBMED    11114166
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Dec 19. 97(26):14340-5."
COMMENT     Sequence Label: pcDNA3-TOP luciferase
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(139..158)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(140..158)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(203..406)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(232..252)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        407..786
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     958..977
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatttg gatccagtgg cggatggcga
       61 cggattctca cgggcacagg agaagagcga ggggcgccga cgatggcggc ggctgcggcg
      121 gaagagaagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
      181 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
      241 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
      301 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
      361 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
      421 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
      481 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
      541 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
      601 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
      661 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
      721 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
      781 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
      841 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
      901 cgcactactc agcgacctcc aacacacaag caggagcaga tactggctta acctatgcgg
      961 catcagagca gattgtact
//
