LOCUS       sequence-328280-        6350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic transgenesis Mini-white physical eye marker cassette in 
            Alpha1, lacks attB site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pMiniwhiteA1
FEATURES             Location/Qualifiers
     source          1..6350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(74..688,759..890,1094..1409,1471..2125,
                     2200..2473,2875..2946))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     3398..3416
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     rep_origin      complement(3451..3906)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3588..3609)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3800..3819
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(4020..4835)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4020..4835)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4743..4762
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(4836..4938)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      5053..5641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5542..5561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcaataaac agtaaacacg atggtaataa tggtaaaaaa aaaaaacaag cagttatttc
       61 ggatatatgt cggctactcc ttgcgtcggg cccgaagtct tagagccaga tatgcgagca
      121 cccggaagct cacgatgaga atggccagac ccacgtagtc cagcggcaga tcggcggcgg
      181 agaagttaag tgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg
      241 tgcagctaat ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg
      301 cgtaacggaa ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt
      361 tcaagaagaa gccgccaaag agcaggaatg gtatgataac cggcggaccc acagacagcg
      421 ccatcgaggt cgaggagctg gcgcaggata ttagatatcc gaaggacgtt gacacattgg
      481 ccaccagagt gaccagcgcc aggcagttga agaagtgcag cactccggcc cgcagtccga
      541 tcatcggata ggcaatcgcc gtgaagacca gtggcactgt gagaaaaagc ggcaattcgg
      601 caatcgtttt gcccagaaag tatgtgtcac agcgataaag tcgacttcgg gcctccctca
      661 taaaaactgg cagctctgag gtgaacacct aaatcgaatc gattcattag aaagttagta
      721 aattattgaa atgcaaatgt attctaaaca tgacttacat ttatcgtggc aaagacgttt
      781 tgaaaggtca tgttggtcag gaagaggaag atggctccgt tgatattcat cacacccact
      841 tgcgtgagtt gttggcccaa aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta
      901 aaatgttact cgcatctcat taatattcgc gagttaaatg aaatttattt atcttctgca
      961 aaactataaa ctatacatct cattgaaaaa aactaagaag ggtgtggaat caggcaattc
     1021 tatctaaaat ctagcgaatt tgtttccaag aattgtaagc gttatatcat ttgtttccac
     1081 tggaaccact caccgttgtc tgaataagtc gcacttttac gaggagtggt tccttgagca
     1141 ccgacagcca ggatcgccac aggaccgccc ggaactgcat gaaccaggtg gccttgtagg
     1201 tgtacccatt ctccggctgc tccagtggct tctccagatt tttggtggcc aacaactgct
     1261 ccatatcccg ggctactttg ctaatggcaa aattgtcgca tatcttggcg atccgatcac
     1321 gggactcgat ctcccgtccg ggcacaacgg ccaacacctg tacgtaaaag tccgccggat
     1381 tgtagttggt aggacactgg gcacccacgc tggataggag ttgagatgtt atgtaatact
     1441 agataccctt aataaacaca tcgaactcac taggaaaaga agtcgacggc ttcgctggga
     1501 gtgcccaaga aagctaccct gccctcggcc atcagaagga tcttgtcaaa gagctcaaac
     1561 agctcggaag acggctgatg aatggtcagg atgacggtct tgcccttctg cgacagcttc
     1621 ttcagcacct ggacgacgct gtgggcggta aaggagtcca gtccggaggt gggctcatcg
     1681 cagatcagaa gcggcggatc ggttagagcc tcggaggcga atgccagacg cttcctttct
     1741 ccgccggaca gacctttcac cctgccgggc acaccgatga tcgtgtgctg acatttgctg
     1801 agcgaaagct cctggatcac ctgatccacg cgggccactc gctgccgata ggtcagatgt
     1861 cgtggcatcc gcaccatggc ttggaaaatc aggtgttccc tggccgttag ggagccgata
     1921 aagaggtcat cctgctggac ataggcgcac ctggcctgca tctccttggc gtccacaggt
     1981 tggccattga gcagtcgcat cccggatggc gatacttgga tgccctgcgg cgatcgaaag
     2041 gcaagggcat tcagcagggt cgtctttccg gcaccggaac tgcccatcac ggccaaaagt
     2101 tcgcccggat aggccacgcc gcaaactgag tttcaaattg gtaattggac cctttattaa
     2161 gatttcacac agatcagccg actgcgaata gaaactcacc gttcttgagc aaatgtttcc
     2221 tgggcgccgg tatgtgtcgc tcgttgcaga atagtccgcg tgtccggttg accagctgcc
     2281 gccatccgga gcccggctga ttgaccgccc caaagatgtc catattgtgc caggcatagg
     2341 tgaggttctc ggctagttgg ccgctccctg aaccggagtc ctccggcgga ctgggtggcc
     2401 ggagcgtgcc gtagtttttg gcctgcccga agccctggtt aatgcagctc tgcgaagccg
     2461 ctccgctgtc accctgcaat gataggggat ctcaaatatc aactacaagc gttatgctca
     2521 tctaaccccg aacaaaaagt accccgaagt atcctacgaa gtaggtttat acttttattt
     2581 attttttgtg catctaggat cagcttaaaa tatctggttg ttatattttt tgtaaaaaag
     2641 aatatagtcg aaaatgaatg cctttagatg tcttgatcat gatatgatct caaaaattgt
     2701 cttatatagc gagaacagct accagaataa tctgtttcgt gtcactattt gtttgtgcaa
     2761 ttgcggtttg ggatttttgt gggtcgcagt tctcacgccg cagacaattt gatgttgcaa
     2821 tcgcagttcc tatagatcaa gtgaacttaa gatgtatgca catgtactac tcacattgtt
     2881 cagatgctcg gcagatgggt gtttgctgcc tccgcgaatt aatagctcct gatcctcttg
     2941 gcccattgcc gggatttttc acactttccc ctgcttaccc acccaaaacc aatcaccacc
     3001 ccaatcactc aaaaaacaaa caaaaataag aagcgagagg agttttggca cagcactttg
     3061 tgtttaattg atggcgtaaa ccgcttggag cttcgtcacg aaaccgctga caaaatgcaa
     3121 ctgaaggcgg acattgacgc tacgtaacgc tacaaacggt ggcgaaagag atagcggacg
     3181 cagcggcgaa agtgacggcg atatttctgt ggacagagaa ggaggcaaac agcgctgact
     3241 ttgagtggaa tgtcattttg agtgagaggt aatcgaaaga acctggtaca tcaaataccc
     3301 ttggatcgaa gtaaatttaa aactgatcag ataagttcaa tgatatccag tgcagtaaaa
     3361 aaaaaaaatg ttttttttat ctactttccg caaaaatggg ttttattaac ttacatacat
     3421 actagaattc ctcctgagac gaattcgtta aaattgtaag cgttaatatt ttgttaaaat
     3481 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3541 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3601 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3661 gcgctggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3721 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3781 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3841 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3901 gcgcgtaaca tcatgtttga cagcttatca tcgaatttct gccattcatc cgcttattat
     3961 cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct taaaaaaaat
     4021 cagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     4081 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     4141 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     4201 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     4261 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     4321 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     4381 gcctgagcga gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     4441 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     4501 tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca
     4561 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     4621 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     4681 aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga ttgcccgaca
     4741 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     4801 cttgagcaag acgtttcccg ttgaatatgg ctcattttag cttccttagc tcctgaaaat
     4861 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     4921 cctcttacgt gccgatcatc acaaaaggat ctaggtgaag atcctttttg ataatctcat
     4981 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5041 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5101 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5161 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5221 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5281 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5341 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5401 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5461 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5521 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5581 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5641 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5701 gttctttcct gcgttatccc ctgattctgt ggataaccgt gtcagaattc gtctcatgac
     5761 agcgatgcat ctacacaagg aacaaacact ggatgtcact ttcagttcaa attgtaacgc
     5821 taatcactcc gaacaggtca caaaaaatta ccttaaaaag tcataatatt aaattagaat
     5881 aaatatagct gtgagggaaa tatatacaaa tatattggag caaataaatt gtacatacaa
     5941 atatttatta ctaatttcta ttgagccgaa atgaaccact cggaaccatt tgagcgaacc
     6001 gaatcgcgcg gaactaacga cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg
     6061 cggagagcgg gtgggagaca gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa
     6121 ttatgaagag ggcgcgcgat ttgaaaagta tgtatataaa aaatatatcc cggtgtttta
     6181 tgtagcgata aacgagtttt tgatgtaagg tatgcaggtg tgtaagtctt ttggttagaa
     6241 gacaaatcca aagtctactt gtggggatgt tcgaagggga aatacttgta ttctataggt
     6301 catatcttgt ttttattggc acaaatataa ttacattagc tttttgaggg
//
