LOCUS       sequence-328281-       10669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G418 resistant QF driver vector with Mini-white CDS eye marker. 
            Contains an enhancer grammar GB20 entry point for custom enhancers. 
            Standard cut-and-paste cloning can also be used. Vector uses 
            Purple-White bacteria colony screening for identification of correct
            cloning .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 10669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pG418R-MCS-QF-MiniWhite
FEATURES             Location/Qualifiers
     source          1..10669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(80..99)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1056..1847
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1902..2357
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1989..2008)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2199..2220
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    2480..2549
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        2577..3028
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2976..2997
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     promoter        3977..3995
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3977..3994
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4006..4023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      4831..4925
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    4932..4988
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(5050..5068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6339..6473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6363..6382)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6417..6436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6561..6579)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7031..7102,7504..7777,7852..8506,8568..8883,9087..9218,
                     9289..9903)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 tttccatagg ctccgccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
       61 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      421 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      661 atgagattat caaaaaggat cttcacctag atccttttgt gaccagccag gacagaaatg
      721 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
      781 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
      841 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
      901 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
      961 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1021 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     1081 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1141 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     1201 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     1261 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     1321 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     1381 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     1441 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     1501 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     1561 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     1621 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtagctg
     1681 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     1741 aggcttatct tggacaagaa gaagatcgtt tggcctcgcg cgcagatcag ttggaagaat
     1801 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatca ctagaccaat
     1861 gttacacata tatactttag attgatttaa aacttcatgt tacgcgccct gtagcggcgc
     1921 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     1981 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     2041 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     2101 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccagcgccct gatagacggt
     2161 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     2221 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     2281 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     2341 attaacgctt acaattttaa cggatcagcg cagctgcccg ggtctagagt cgacgcatgc
     2401 ctcctgacag cgagatgtcc taatatggac atcctctccg gatcccaggg aggaattggt
     2461 cgacgatgta ggtcactgtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct
     2521 ccccgggcgc gtactccacc tcacccatct ggtccatcat gatgcgctgt caggagctag
     2581 aatcccaaaa caaactggtt attgtggtag gtcatttgtt tggcagaaag aaaactcgag
     2641 aaatttctct ggccgttatt cgttattctc tcttttcttt ttgggtcgct ccctctctgc
     2701 actaatgctc tctcactctg tcacacagta aacggcatac tgctctcgtt ggttcgagag
     2761 agcgcgcctc gaatgttcgc gaaaagagcg ccggagtata aatagaggcg cttcgtctac
     2821 ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa
     2881 ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat
     2941 caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag
     3001 taattattga atacaagaag agaactctca atactcgaat ccatgtggga gtttattctt
     3061 gacacagata tttatgatat aataactgag taagcttaac ataaggagga aaacaaaatg
     3121 attgagcagg atggactgca cgctggctcg ccagctgcct gggtggagcg tctgttcggc
     3181 tacgattggg cccagcagac catcggctgc tccgatgctg ccgtgttccg tctgtcggcc
     3241 cagggacgcc ccgtgctgtt cgtcaagacc gacctgagtg gagccctgaa cgagctccag
     3301 gatgaggcag ctcgtctgag ctggctggcc acaacaggag tgccgtgtgc tgccgtgctg
     3361 gatgtggtga ccgaagctgg acgcgattgg ctgctgctgg gcgaggtgcc aggccaggac
     3421 ctgctgtcca gccatctggc cccagccgag aaggtgtcca ttatggccga tgctatgcgt
     3481 cgcctgcaca ccctggaccc agccacctgc ccctttgacc accaggccaa gcaccgtatt
     3541 gagcgtgccc gaacccgtat ggaggctgga ctggtggacc aggatgacct ggatgaggag
     3601 caccagggcc tggctccagc cgagctgttt gcccgtctga aggcccgtat gcccgatggc
     3661 gaggacctgg tggtgaccca cggcgacgcc tgcctgccca acattatggt ggagaacgga
     3721 cgcttcagtg gcttcattga ttgtggacgc ctgggcgtgg ccgaccgtta ccaggatatc
     3781 gccctggcca cccgagatat cgccgaggag ctgggaggcg agtgggccga ccgattcctg
     3841 gtgctctacg gcattgcagc cccagatagc cagcgtattg ccttctaccg tctgctggac
     3901 gagttcttct aagcttggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     3961 gttccgctgt caggagattt aggtgacact atagacttcc gcttcctcgc tcactgactc
     4021 gctgcgctcg gtcgttcggc tgtcgggaga gagaccaatc catgtgggag tttattcttg
     4081 acacagatat ttatgatata ataactgagt aagcttaaca taaggaggaa aacaaaatgg
     4141 cgagcttggt taagaaagat atgtgtgtta agatgacgat ggaaggtact gtgaacggtt
     4201 atcactttaa gtgcgttggc gagggtgaag gcaagccgtt cgagggcacg cagaacatgc
     4261 gcattcgtgt caccgagggc ggtccgctgc cttttgcatt cgacatcctg gccccgtgct
     4321 gtatgtacgg ctctaagacc ttcattaaac acgtgagcgg tatcccggat tactttaaag
     4381 agtcctttcc agagggcttc acttgggaac gtacccagat ttttgaggac ggtggtgttc
     4441 tgaccgcgca ccaagacacc agcctggaag gtaattgcct gatctataaa gtgaaggttc
     4501 tgggtaccaa tttcccggcg aatggtccgg tgatgcaaaa gaaaaccgcg ggttgggagc
     4561 cgtgcgtcga gatgctgtat ccgcgtgacg gcgtcttgtg tggtcagagc ttgatggcgc
     4621 tgaagtgcac cgatggcaat catctgacca gccacctgcg cacgacgtat cgtagccgta
     4681 aaccgagcaa cgccgttaac atgccggagt tccattttgg tgaccatcgc atcgaaatcc
     4741 tgaaagctga gcagggcaaa ttctacgaac aatacgaatc ggctgtcgca cgttacagcg
     4801 atgtgccgga aaaagcgacg taatgagctt gactcctgtt gatagatcca gtaatgacct
     4861 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
     4921 agaattactt caagcttgca tgcctgcagg tcgactctag aggatccccg ggtaccgagc
     4981 tcgaattcgg tctcttcccg cttgtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     5041 cgcatcaggc ctatagtgag tcgtattatc ccgagctcgc ccggggatcg agcgcagcgg
     5101 tataaaaggg cgcggggtgg ctgagagcat cagttgtgaa tgaatgttcg agccgagcag
     5161 acgtgccgct gccttcgtta atatcctttg aataagccaa ctttgaatca caagacgcat
     5221 accaaaccaa atgcccccca agcgcaagac tctgaacgcc gccgctgagg ccaacgccca
     5281 cgctgacggt catgctgatg gtaacgctga tggtcacgtc gccaataccg ctgctagctc
     5341 caacaacgcc cgtttcgccg acctgaccaa tattgatacc cccggcttgg gccccaccac
     5401 caccaccctg ctggtggagc ccgcccgttc caagcgtcag cgtgtgtccc gcgcctgcga
     5461 ccagtgccgc gccgctcgtg agaagtgcga cggaatccag ccagcctgct tcccctgcgt
     5521 gtcccagggc cgctcctgca cttaccaggc ctccccaaaa aagcgcggcg tgcagactgg
     5581 atacattcgc accctcgagc tcgccctggc ctggatgttc gagaacgtgg cccgctccga
     5641 ggatgctctg cataacctgt tggtccgtga cgccggacag ggctcggccc tgctggtcgg
     5701 caaggactcg cccgccgctg agcgcctgca tgctcgttgg gccaccagcc gtgtcaacaa
     5761 gagcatcacc cgcctcctga gtggcggagg ttccggtggc ggcagcggcg ggggttcggg
     5821 aggtggctcg cgccagctgg agctgccacc cactgccacc gccaccgctt cgattatgcc
     5881 acacgtcatg gagcagccgc tgtccacctc catcaacccc gtcaacgatc gcttcaacgg
     5941 catcccaaac cccaccccct acaactccga cgccgccctg gacgccatca cccagaccaa
     6001 cgactacggt tccgtcaata cccacggcat cttgtccact taccccccgc cagccaccca
     6061 cctcaacgag gcctccgtcg ccctggctcc cggcggagct ccaccccgtc ccccgccccc
     6121 ctacgtcgac tccaccacca accacccgcc ctaccactcc aacctggtgc ccatggctaa
     6181 cttcggatac tccaccgttg actacgatgc catggtggac gatttggctt ccatcgagta
     6241 cactgacgcc gttgacgtcg atccgcagtt catgaccaac ctgggtttcg ttcccggctg
     6301 caacttctcc gacatcaaca cttacgagca gtaagcttga tccagacatg ataagataca
     6361 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     6421 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     6481 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcgctgt
     6541 caggaggaat tctagtatgt atgtaagtta ataaaaccca tttttgcgga aagtagataa
     6601 aaaaaacatt tttttttttt actgcactgg atatcattga acttatctga tcagttttaa
     6661 atttacttcg atccaagggt atttgatgta ccaggttctt tcgattacct ctcactcaaa
     6721 atgacattcc actcaaagtc agcgctgttt gcctccttct ctgtccacag aaatatcgcc
     6781 gtcactttcg ccgctgcgtc cgctatctct ttcgccaccg tttgtagcgt tacgtagcgt
     6841 caatgtccgc cttcagttgc attttgtcag cggtttcgtg acgaagctcc aagcggttta
     6901 cgccatcaat taaacacaaa gtgctgtgcc aaaactcctc tcgcttctta tttttgtttg
     6961 ttttttgagt gattggggtg gtgattggtt ttgggtgggt aagcagggga aagtgtgaaa
     7021 aatcccggca atgggccaag aggatcagga gctattaatt cgcggaggca gcaaacaccc
     7081 atctgccgag catctgaaca atgtgagtag tacatgtgca tacatcttaa gttcacttga
     7141 tctataggaa ctgcgattgc aacatcaaat tgtctgcggc gtgagaactg cgacccacaa
     7201 aaatcccaaa ccgcaattgc acaaacaaat agtgacacga aacagattat tctggtagct
     7261 gttctcgcta tataagacaa tttttgagat catatcatga tcaagacatc taaaggcatt
     7321 cattttcgac tatattcttt tttacaaaaa atataacaac cagatatttt aagctgatcc
     7381 tagatgcaca aaaaataaat aaaagtataa acctacttcg taggatactt cggggtactt
     7441 tttgttcggg gttagatgag cataacgctt gtagttgata tttgagatcc cctatcattg
     7501 cagggtgaca gcggagcggc ttcgcagagc tgcattaacc agggcttcgg gcaggccaaa
     7561 aactacggca cgctccggcc acccagtccg ccggaggact ccggttcagg gagcggccaa
     7621 ctagccgaga acctcaccta tgcctggcac aatatggaca tctttggggc ggtcaatcag
     7681 ccgggctccg gatggcggca gctggtcaac cggacacgcg gactattctg caacgagcga
     7741 cacataccgg cgcccaggaa acatttgctc aagaacggtg agtttctatt cgcagtcggc
     7801 tgatctgtgt gaaatcttaa taaagggtcc aattaccaat ttgaaactca gtttgcggcg
     7861 tggcctatcc gggcgaactt ttggccgtga tgggcagttc cggtgccgga aagacgaccc
     7921 tgctgaatgc ccttgccttt cgatcgccgc agggcatcca agtatcgcca tccgggatgc
     7981 gactgctcaa tggccaacct gtggacgcca aggagatgca ggccaggtgc gcctatgtcc
     8041 agcaggatga cctctttatc ggctccctaa cggccaggga acacctgatt ttccaagcca
     8101 tggtgcggat gccacgacat ctgacctatc ggcagcgagt ggcccgcgtg gatcaggtga
     8161 tccaggagct ttcgctcagc aaatgtcagc acacgatcat cggtgtgccc ggcagggtga
     8221 aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc ctccgaggct ctaaccgatc
     8281 cgccgcttct gatctgcgat gagcccacct ccggactgga ctcctttacc gcccacagcg
     8341 tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac cgtcatcctg accattcatc
     8401 agccgtcttc cgagctgttt gagctctttg acaagatcct tctgatggcc gagggcaggg
     8461 tagctttctt gggcactccc agcgaagccg tcgacttctt ttcctagtga gttcgatgtg
     8521 tttattaagg gtatctagta ttacataaca tctcaactcc tatccagcgt gggtgcccag
     8581 tgtcctacca actacaatcc ggcggacttt tacgtacagg tgttggccgt tgtgcccgga
     8641 cgggagatcg agtcccgtga tcggatcgcc aagatatgcg acaattttgc cattagcaaa
     8701 gtagcccggg atatggagca gttgttggcc accaaaaatc tggagaagcc actggagcag
     8761 ccggagaatg ggtacaccta caaggccacc tggttcatgc agttccgggc ggtcctgtgg
     8821 cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa aagtgcgact tattcagaca
     8881 acggtgagtg gttccagtgg aaacaaatga tataacgctt acaattcttg gaaacaaatt
     8941 cgctagattt tagatagaat tgcctgattc cacacccttc ttagtttttt tcaatgagat
     9001 gtatagttta tagttttgca gaagataaat aaatttcatt taactcgcga atattaatga
     9061 gatgcgagta acattttaat ttgcagatgg ttgccatctt gattggcctc atctttttgg
     9121 gccaacaact cacgcaagtg ggtgtgatga atatcaacgg agccatcttc ctcttcctga
     9181 ccaacatgac ctttcaaaac gtctttgcca cgataaatgt aagtcatgtt tagaatacat
     9241 ttgcatttca ataatttact aactttctaa tgaatcgatt cgatttaggt gttcacctca
     9301 gagctgccag tttttatgag ggaggcccga agtcgacttt atcgctgtga cacatacttt
     9361 ctgggcaaaa cgattgccga attgccgctt tttctcacag tgccactggt cttcacggcg
     9421 attgcctatc cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg
     9481 ctggtcactc tggtggccaa tgtgtcaacg tccttcggat atctaatatc ctgcgccagc
     9541 tcctcgacct cgatggcgct gtctgtgggt ccgccggtta tcataccatt cctgctcttt
     9601 ggcggcttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc
     9661 tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg
     9721 ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg
     9781 gagacactta acttctccgc cgccgatctg ccgctggact acgtgggtct ggccattctc
     9841 atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag
     9901 tagccgacat atatccgaaa taactgcttg tttttttttt ttaccattat taccatcgtg
     9961 tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa
    10021 gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga
    10081 tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc
    10141 gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct
    10201 tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc
    10261 tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg
    10321 cgattcggtt cgctcaaatg gttccgagtg gttcatttcg gctcaataga aattagtaat
    10381 aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc cctcacagct
    10441 atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac ctgttcggag
    10501 tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt gtgtagatgc
    10561 atcgcttgac acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10621 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgtt
//
