LOCUS       sequence-32829-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Kim JE, Chen J
  TITLE     Cytoplasmic-nuclear shuttling of FKBP12-rapamycin-associated protein
            is involved in rapamycin-sensitive signaling and translation 
            initiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Dec 19. 97(26):14340-5.
  PUBMED    11114166
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Dec 19. 97(26):14340-5."
COMMENT     Sequence Label: pcDNA3 luciferase
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(170..188)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctcggatc caaatggaag acgccaaaaa
      121 cataaagaaa ggcccggcgc cattctatcc tctagaggat ggaaccgctg gagagcaact
      181 gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca
      241 tatcgaggtg aacatcacgt acgcggaata cttcgaaatg tccgttcggt tggcagaagc
      301 tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct
      361 tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa
      421 cgacatttat aatgaacgtg aattgctcaa cagtatgaac atttcgcagc ctaccgtagt
      481 gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat taccaataat
      541 ccagaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac
      601 gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtac cagagtcctt
      661 tgatcgtgac aaaacaattg cactgataat gaattcctct ggatctactg ggttacctaa
      721 gggtgtggcc cttccgcata gaactgcctg cgtcagattc tcgcatgcca gagatcctat
      781 ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg
      841 ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta
      901 tagatttgaa gaagagctgt ttttacgatc ccttcaggat tacaaaattc aaagtgcgtt
      961 gctagta
//
