LOCUS       sequence-328329-        9862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R 
            mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and 
            inflammasome activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R
FEATURES             Location/Qualifiers
     source          1..9862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      929..938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3242..3253
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5354..5377
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5415..5433)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5416..5433)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5453..5470)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5459..5683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5729..6157
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5816..5835)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6026..6047
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6166..6186)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6171..6500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6351..6486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6413..6432
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6567..7361
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6621..6640)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7231..7250
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7535..7656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7572..7591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7626..7645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7705..7721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7705..7721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7718..7740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7729..7745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7753..7759,7760..7777,7778..7783))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7798..7819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7936..7953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8107..8695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8187..8206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8866..9657,9658..9726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9489..9508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9727..9831)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccctacttcc aatccaatgc caccatggct ggcggagcct ggggccgcct
      961 ggcctgttac ttggagttcc tgaagaagga ggagctgaag gagttccagc ttctgctcgc
     1021 caataaagcg cactccagga gctcttcggg tgagacaccc gctcagccag agaagacgag
     1081 tggcatggag gtggcctcgt acctggtggc tcagtatggg gagcagcggg cctgggacct
     1141 agccctccat acctgggagc agatggggct gaggtcactg tgcgcccaag cccaggaagg
     1201 ggcaggccac tctccctcat tcccctacag cccaagtgaa ccccacctgg ggtctcccag
     1261 ccaacccacc tccaccgcag tgctaatgcc ctggatccat gaattgccgg cggggtgcac
     1321 ccagggctca gagagaaggg ttttgagaca gctgcctgac acatctggac gccgctggag
     1381 agaaatctct gcctcactcc tctaccaagc tcttccaagc tccccagacc atgagtctcc
     1441 aagccaggag tcacccaacg cccccacatc cacagcagtg ctggggagct ggggatcccc
     1501 acctcagccc agcctagcac ccagagagca ggaggctcct gggacccaat ggcctctgga
     1561 tgaaacgtca ggaatttact acacagaaat cagagaaaga gagagagaga aatcagagaa
     1621 aggcaggccc ccatgggcag cggtggtagg aacgccccca caggcgcaca ccagcctaca
     1681 gccccaccac cacccatggg agccttctgt gagagagagc ctctgttcca catggccctg
     1741 gaaaaatgag gattttaacc aaaaattcac acagctgcta cttctacaaa gacctcaccc
     1801 cagaagccaa gatcccctgg tcaagagaag ctggcctgat tatgtggagg agaatcgagg
     1861 acatttaatt gagatcagag acttatttgg cccaggcctg gatacccaag aacctcgcat
     1921 agtcatactg cagggggctg ctggaattgg gaagtcaaca ctggccaggc aggtgaagga
     1981 agcctggggg agaggccagc tgtatgggga ccgcttccag catgtcttct acttcagctg
     2041 cagagagctg gcccagtcca aggtggtgag tctcgctgag ctcatcggaa aagatgggac
     2101 agccactccg gctcccatta gacagatcct gtctaggcca gagcggctgc tcttcatcct
     2161 cgatggtgta gatgagccag gatgggtctt gcaggagccg agttctgagc tctgtctgca
     2221 ctggagccag ccacagccgg cggatgcact gctgggcagt ttgctgggga aaactatact
     2281 tcccgaggca tccttcctga tcacggctcg gaccacagct ctgcagaacc tcattccttc
     2341 tttggagcag gcacgttggg tagaggtcct ggggttctct gagtccagca ggaaggaata
     2401 tttctacaga tatttcacag atgaaaggca agcaattaga gcctttaggt tggtcaaatc
     2461 aaacaaagag ctctgggccc tgtgtcttgt gccctgggtg tcctggctgg cctgcacttg
     2521 cctgatgcag cagatgaagc ggaaggaaaa actcacactg acttccaaga ccaccacaac
     2581 cctctgtcta cattaccttg cccaggctct ccaagctcag ccattgggac cccagctcag
     2641 agacctctgc tctctggctg ctgagggcat ctggcaaaaa aagacccttt tcagtccaga
     2701 tgacctcagg aagcatgggt tagatggggc catcatctcc accttcttga agatgggtat
     2761 tcttcaagag caccccatcc ctctgagcta cagcttcatt cacctctgtt tccaagagtt
     2821 ctttgcagca atgtcctatg tcttggagga tgagaagggg agaggtaaac attctaattg
     2881 catcatagat ttggaaaaga cgctagaagc atatggaata catggcctgt ttggggcatc
     2941 aaccacacgt ttcctattgg gcctgttaag tgatgagggg gagagagaga tggagaacat
     3001 ctttcactgc cggctgtctc aggggaggaa cctgatgcag tgggtcccgt ccctgcagct
     3061 gctgctgcag ccacactctc tggagtccct ccactgcttg tacgagactc ggaacaaaac
     3121 gttcctgaca caagtgatgg cccatttcga agaaatgggc atgtgtgtag aaacagacat
     3181 ggagctctta gtgtgcactt tctgcattaa attcagccgc cacgtgaaga agcttcagct
     3241 gattgagggc aggcagcaca gatcaacatg gagccccacc atggtagtcc tgttcaggtg
     3301 ggtcccagtc acagatgcct attggcagat tctcttctcc gtcctcaagg tcaccagaaa
     3361 cctgaaggag ctggacctaa gtggaaactc gctgagccac tctgcagtga agagtctttg
     3421 taagaccctg agacgccctc gctgcctcct ggagaccctg cggttggctg gctgtggcct
     3481 cacagctgag gactgcaagg accttgcctt tgggctgaga gccaaccaga ccctgaccga
     3541 gctggacctg agcttcaatg tgctcacgga tgctggagcc aaacaccttt gccagagact
     3601 gagacagccg agctgcaagc tacagcgact gcagctggtc agctgtggcc tcacgtctga
     3661 ctgctgccag gacctggcct ctgtgcttag tgccagcccc agcctgaagg agctagacct
     3721 gcagcagaac aacctggatg acgttggcgt gcgactgctc tgtgaggggc tcaggcatcc
     3781 tgcctgcaaa ctcatacgcc tggggctgga ccagacaact ctgagtgatg agatgaggca
     3841 ggaactgagg gccctggagc aggagaaacc tcagctgctc atcttcagca gacggaaacc
     3901 aagtgtgatg acccctactg agggcctgga tacgggagag atgagtaata gcacatcctc
     3961 actcaagcgg cagagactcg gatcagagag ggcggcttcc catgttgctc aggctaatct
     4021 caaactcctg gacgtgagca agatcttccc aattgctgag attgcagagg aaagctcccc
     4081 agaggtagta ccggtggaac tcttgtgcgt gccttctcct gcctctcaag gggacctgca
     4141 tacgaagcct ttggggactg acgatgactt ctggggcccc acggggcctg tggctactga
     4201 ggtagttgac aaagaaaaga acttgtaccg agttcacttc cctgtagctg gctcctaccg
     4261 ctggcccaac acgggtctct gctttgtgat gagagaagcg gtgaccgttg agattgaatt
     4321 ctgtgtgtgg gaccagttcc tgggtgagat caacccacag cacagctgga tggtggcagg
     4381 gcctctgctg gacatcaagg ctgagcctgg agctgtggaa gctgtgcacc tccctcactt
     4441 tgtggctctc caagggggcc atgtggacac atccctgttc caaatggccc actttaaaga
     4501 ggaggggatg ctcctggaga agccagccag ggtggagctg catcacatag ttctggaaaa
     4561 ccccagcttc tcccccttgg gagtcctcct gaaaatgatc cataatgccc tgcgcttcat
     4621 tcccgtcacc tctgtggtgt tgctttacca ccgcgtccat cctgaggaag tcaccttcca
     4681 cctctacctg atcccaagtg actgctccat tcggaaggcc atagatgatc tagaaatgaa
     4741 attccagttt gtgcgaatcc acaagccacc cccgctgacc ccactttata tgggctgtcg
     4801 ttacactgtg tctgggtctg gttcagggat gctggaaata ctccccaagg aactggagct
     4861 ctgctatcga agccctggag aagaccagct gttctcgcgg ttctacgttg gccacttggg
     4921 atcagggatc aggctgcaag tgaaagacaa gaaagatgag actctggtgt gggaggcctt
     4981 ggtgaaacca ggagatctca tgcctgcaac tactctgatc cctccagccc gcatagccgt
     5041 accttcacct ctggatgccc cgcagttgct gcactttgtg gaccagtatc gagagcagct
     5101 gatagcccga gtgacatcgg tggaggttgt cttggacaaa ctgcatggac aggtgctgag
     5161 ccaggagcag tacgagaggg tgctggctga gaacacgagg cccagccaga tgcggaagct
     5221 gttcagcttg agccagtcct gggaccggaa gtgcaaagat ggactctacc aagccctgaa
     5281 ggagacccat cctcacctca ttatggaact ctgggagaag ggcagcaaaa agggactcct
     5341 gccactcagc agcgactaca aagacgatga cgacaagtaa taacattgga agtggataat
     5401 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
     5461 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5521 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5581 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5641 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5701 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5761 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5821 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5881 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5941 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6001 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6061 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6121 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     6181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6541 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6601 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6661 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6721 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6781 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6841 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6901 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6961 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7021 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7081 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     7141 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7201 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7261 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7321 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     7381 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7441 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7501 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7681 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7741 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7801 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7861 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8521 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9721 actcatactc ttcctttttc attattattg aagcatttat cagggttatt gtctcatgag
     9781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9841 ccgaaaagtg ccacctgacg tc
//
