LOCUS       sequence-328358-        1443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for human VPS4A ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Neggers JE, Paolella BR, Asfaw A, Rothberg MV, Skipper TA, Yang A, 
            Kalekar RL, Krill-Burger JM, Dharia NV, Kugener G, Kalfon J, Yuan C,
            Dumont N, Gonzalez A, Abdusamad M, Li YY, Spurr LF, Wu WW, Durbin 
            AD, Wolpin BM, Piccioni F, Root DE, Boehm JS, Cherniack AD, 
            Tsherniak A, Hong AL, Hahn WC, Stegmaier K, Golub TR, Vazquez F, 
            Aguirre AJ
  TITLE     Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A
            and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.
  JOURNAL   Cell Rep. 2020 Dec 15;33(11):108493. doi: 
            10.1016/j.celrep.2020.108493.
  PUBMED    33326793
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-12-15"; volume: "33(11):108493. doi"; pages: " 
            10.1016/j.celrep.2020.108493"
COMMENT     Sequence Label: pDONR 223 VPS4A
FEATURES             Location/Qualifiers
     source          1..1443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1378..1402)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
       61 ttggcatgac aacgtcaacc ctccagaaag ccattgatct ggtgacgaaa gccacagagg
      121 aggacaaagc caagaactac gaggaggcgc tgcggctgta ccagcatgcg gtggagtact
      181 tcctccacgc tatcaagtat gaggcccaca gcgacaaggc caaggagagc attcgagcca
      241 agtgcgtgca gtacctagac cgggccgaga agctgaagga ttatttacga agcaaagaga
      301 aacacggcaa gaagccagtc aaagagaacc agagtgaggg caagggcagt gacagtgaca
      361 gtgaagggga taatccggag aaaaagaaac tgcaagaaca gctgatgggt gccgtcgtga
      421 tggagaagcc caacatacgg tggaacgacg tggccgggct ggagggggcc aaggaggccc
      481 tcaaagaagc tgtcattttg ccaatcaaat tcccacactt gttcacaggc aagcgcaccc
      541 cctggcgggg gattctgctg ttcggacccc ctggcacagg gaaatcctac ctggccaaag
      601 ccgtggcaac agaggccaac aactccacct tcttctctgt gtcctcctca gatctgatgt
      661 ccaagtggct gggggagagt gaaaagctgg tcaagaacct gtttgagctg gccaggcagc
      721 acaagccctc catcatcttc atcgatgagg tggattccct ctgcgggtcc cgaaatgaaa
      781 atgagagtga ggccgcccgg aggatcaaaa cggagttctt ggtccagatg cagggggtgg
      841 ggaataacaa tgatgggact ctggttcttg gagccacaaa catcccatgg gtgttggatt
      901 cggccatcag gaggaggttt gaaaaacgaa tttatatccc cttgccggag gaagctgccc
      961 gcgcccagat gttccggttg catctcggga gcactcccca caacctcacg gatgcaaaca
     1021 tccacgagct ggcccggaag acggaaggct actcgggcgc ggacatcagc atcatcgtgc
     1081 gggactctct catgcagccc gtgaggaagg tgcagtcggc cacacacttc aaaaaggtct
     1141 gtggcccctc tcgcaccaac cccagcatga tgattgatga cctcctgact ccatgctcac
     1201 caggggaccc aggagccatg gagatgactt ggatggatgt ccctggggac aaactcttag
     1261 agcctgtggt ttgcatgtcg gacatgctgc ggtctctggc caccacccgg cccacggtga
     1321 atgcagacga cctcctgaaa gtgaagaaat tctcagagga ctttgggcaa gagagttacc
     1381 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1441 aac
//
