LOCUS       sequence-32836-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Drevets DA, Jelinek TA, Freitag NE
  TITLE     Listeria monocytogenes-infected phagocytes can initiate central 
            nervous system infection in mice.
  JOURNAL   Infect Immun. 2001 Mar . 69(3):1344-50.
  PUBMED    11179297
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun. 2001 Mar . 69(3):1344-50."
COMMENT     Sequence Label: pNF579
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(55..71)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(55..71)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(68..90)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    79..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(103..109,110..127,128..133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    148..169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tatttttcct ccttctagag gatccccggg taccgagctc gaattcgtaa tcatgtcata
       61 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      121 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      181 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctggactaaa aggcatgcaa
      241 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
      301 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
      361 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
      421 gagttgctag taacatctga ccgagatttt tttgagcaac tggatcagta caagaaagat
      481 actgtatttc ataaacagga actgcaagaa gttaaggatg agttacagaa ggcaaataag
      541 cagttacaga gtggaataga gcatatgagg tctacgaaac cctttgatta tgaaaatgag
      601 cgtacaggtt tgttctctgg acgtgaagag actggtagaa agatattaac tgctgatgaa
      661 tttgaacgcc tgcaagaaac aatctcttcg aacggattgt tgatgattac gaaaatatta
      721 agagcacaga ctattacaca gaaaatcaag aattaaaaaa acgtagagag agtttgaaag
      781 aagtagtgaa tacatggaaa gaggggtatc acgaaaaaag taaagaggtt aataaattaa
      841 agcgagagaa tgatagtttg aatgagcagt tgaatgta
//
