LOCUS       sequence-328364-        1464 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for human VPS4B ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Neggers JE, Paolella BR, Asfaw A, Rothberg MV, Skipper TA, Yang A, 
            Kalekar RL, Krill-Burger JM, Dharia NV, Kugener G, Kalfon J, Yuan C,
            Dumont N, Gonzalez A, Abdusamad M, Li YY, Spurr LF, Wu WW, Durbin 
            AD, Wolpin BM, Piccioni F, Root DE, Boehm JS, Cherniack AD, 
            Tsherniak A, Hong AL, Hahn WC, Stegmaier K, Golub TR, Vazquez F, 
            Aguirre AJ
  TITLE     Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A
            and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.
  JOURNAL   Cell Rep. 2020 Dec 15;33(11):108493. doi: 
            10.1016/j.celrep.2020.108493.
  PUBMED    33326793
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-12-15"; volume: "33(11):108493. doi"; pages: " 
            10.1016/j.celrep.2020.108493"
COMMENT     Sequence Label: pDONR 223 VPS4B
FEATURES             Location/Qualifiers
     source          1..1464
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             889..900
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1399..1423)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
       61 ttggcatgtc atccacttcg cccaacctcc agaaagcgat agatctggct agcaaagcag
      121 cgcaagaaga caaggctggg aactacgaag aagcccttca gctctatcag catgctgtgc
      181 agtattttct tcatgtcgtt aaatatgaag cacagggtga taaagccaag caaagtatca
      241 gggcaaagtg tacagaatat cttgatagag cagaaaaact aaaggagtac ctgaaaaata
      301 aagagaaaaa agcacagaag ccagtgaaag aaggacagcc gagtccagca gatgagaagg
      361 ggaatgacag tgatggggaa ggagaatctg atgatcctga aaaaaagaaa ctacagaatc
      421 aacttcaagg tgccattgtt atagaacgac caaatgtgaa atggagtgac gttgctggac
      481 ttgaaggagc caaagaagca ctgaaagagg ctgtgatact gcctattaaa tttcctcatc
      541 tttttacagg caagagaaca ccttggaggg gaatcctatt atttgggccg cctggaacag
      601 gaaagtccta cttagccaaa gctgtagcaa cagaagccaa caactcaaca tttttttcaa
      661 tatcttcctc tgatcttgtt tctaagtggc taggtgaaag tgaaaaactg gttaagaatt
      721 tattccaact tgccagagag aacaagccct ccattatctt cattgatgaa attgattctc
      781 tctgtggttc aagaagtgaa aatgaaagtg aagccgcacg tagaattaag acggagttcc
      841 tagtgcaaat gcaaggggtt ggtgtagaca atgatggaat tttggttctg ggagctacaa
      901 atataccctg ggttctggat tctgccatta ggcgaagatt tgagaaacga atttatattc
      961 ccttgccgga accccatgcc cgagcagcaa tgtttaaact gcacctaggg accactcaga
     1021 acagtctcac ggaagcagac tttcgggaac ttgggaggaa aacagatggt tattcagggg
     1081 cagatataag tatcattgta cgtgatgccc ttatgcagcc tgttaggaaa gtacagtcag
     1141 ctactcattt taaaaaggtt cgcggacctt cccgagctga tcctaaccat cttgtagatg
     1201 atctgctaac accttgctct ccaggtgacc ctggtgccat tgaaatgaca tggatggatg
     1261 tccctggaga taaacttttg gagccagttg tttccatgtc ggatatgttg cggtcactat
     1321 ctaacacaaa acctacagtc aatgaacatg acttgttgaa attaaagaag tttacagaag
     1381 attttggtca agaaggctac ccaactttct tgtacaaagt tggcattata agaaagcatt
     1441 gcttatcaat ttgttgcaac gaac
//
