LOCUS       sequence-328404-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA3.1 His-NLRP1 codon-optimized
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             920..937
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3242..3253
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5354..5371
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5468..5485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5474..5698
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5744..6172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5831..5850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6041..6062
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6181..6201)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6186..6515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6366..6501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6428..6447
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6582..7376
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6636..6655)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7246..7265
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7550..7671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7587..7606)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7641..7660
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7720..7736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7720..7736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7733..7755)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7744..7760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7768..7774,7775..7792,7793..7798))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7813..7834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7951..7968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8122..8710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8202..8221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8881..9672,9673..9741))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9504..9523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9742..9846)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgc atcatcatca tcatcatgct ggaggagctt ggggaagact
      961 ggcttgctac ctggagttcc tgaagaagga ggagctgaag gagttccagc tgctgctggc
     1021 caacaaagcc cacagcagaa gcagcagcgg agagacacca gcccagccag aaaagaccag
     1081 cggaatggag gtggccagct atctggtggc tcagtacgga gagcagagag cttgggacct
     1141 ggctctgcac acttgggagc agatgggact gcgaagtctc tgcgctcagg ctcaggaagg
     1201 agcaggccac tctcctagct tcccttacag ccctagcgaa cctcacctgg gctctccttc
     1261 tcagcctaca agcaccgcag tgctgatgcc ttggatccac gagctgccag caggttgtac
     1321 ccagggaagc gagagaaggg tgctgagaca gctgccagac acaagcggaa ggcgctggag
     1381 agaaatctca gccagcctgc tgtatcaggc cctgccttct agcccagatc acgagagccc
     1441 tagccaggag tctcctaacg cccctacaag caccgcagtg ctgggatctt ggggatctcc
     1501 tccacagcca tctctggctc ctagagagca ggaggctcca ggaactcagt ggcctctgga
     1561 cgaaaccagc ggcatctact acaccgagat ccgggagaga gagagggaga agagcgagaa
     1621 gggcagacct ccttgggcag cagtcgtggg aacacctcct caggctcaca catctctgca
     1681 gcctcaccac cacccttggg aacctagcgt gagagagagc ctctgcagca cttggccttg
     1741 gaagaacgag gacttcaacc agaagttcac ccagctgctg ctgctgcaga gacctcatcc
     1801 taggagccag gatcctctgg tgaagcggtc ttggccagac tacgtggagg agaacagagg
     1861 ccacctgatc gagatccggg acctgtttgg cccaggactg gatacccagg agcccagaat
     1921 cgtgatcctg cagggagccg ccggaattgg caaaagcaca ctggccagac aggtcaagga
     1981 ggcttgggga agaggacagc tgtacggcga cagattccag cacgtgttct acttctcttg
     2041 ccgggagctg gcccagtcta aagtggtgtc cctggccgag ctgatcggaa aagacggaac
     2101 agccacccca gcccctatca gacagatcct gagcaggcca gagaggctgc tgttcatcct
     2161 ggacggagtc gacgaaccag gttgggtgct gcaggaacca agcagcgagc tctgcctgca
     2221 ttggtctcag cctcagccag cagacgctct cctgggaagc ctgctgggca agacaatcct
     2281 gccagaggcc agcttcctga tcaccgctag aaccaccgcc ctgcagaacc tgatcccttc
     2341 tctggaacag gctcgctggg tggaagtgct gggattcagc gagagcagcc ggaaggagta
     2401 cttctaccgc tacttcaccg acgagaggca ggctatcagg gccttccggc tggtgaagag
     2461 caacaaggag ctgtgggctc tgtgtctggt gccttgggtc tcttggctgg cttgcacttg
     2521 cctgatgcag cagatgaagc ggaaggagaa gctgaccctg accagcaaga caaccaccac
     2581 cctgtgcctg cactatctgg ctcaggctct gcaggctcag cctctgggac ctcagctgag
     2641 agatctgtgt tctctggccg ccgaaggcat ttggcagaag aagaccctgt tcagccccga
     2701 cgacctgaga aagcacggac tggacggagc catcatcagc accttcctga agatgggcat
     2761 cctgcaggag caccctatcc ctctgagcta cagcttcatc cacctctgct tccaggagtt
     2821 cttcgccgcc atgagctacg tgctggaaga cgaaaagggc cggggcaagc acagcaattg
     2881 catcatcgac ctggagaaga ccctggaggc ctacggaatc cacggactgt ttggcgccag
     2941 cacaaccaga ttcctgctgg gcctgctgtc agacgaggga gagcgcgaga tggagaacat
     3001 cttccattgc aggctgagcc agggcagaaa cctgatgcag tgggtgccat ctctgcagct
     3061 gctcctgcag cctcatagcc tggagagcct gcattgcctg tacgagaccc ggaacaagac
     3121 cttcctgacc caggtcatgg cccacttcga ggagatgggc atgtgcgtgg agaccgacat
     3181 ggagctcctc gtctgcacct tttgcatcaa gttcagccgg cacgtgaaga agctgcagct
     3241 gatcgagggc agacagcaca gaagcacttg gagccctacc atggtggtgc tctttcggtg
     3301 ggtcccagtg accgacgcct attggcagat cctgttcagc gtgctgaagg tcacccggaa
     3361 cctgaaggag ctggacctga gcggcaactc tctgtctcac agcgccgtga agagcctgtg
     3421 caaaaccctg aggagacctc gctgtctgct ggaaaccctg agactggcag gttgcggact
     3481 gacagcagag gattgcaagg acctggcctt tggcctgagg gcaaatcaga ccctgaccga
     3541 gctggacctg agcttcaacg tgctgaccga cgcaggagcc aaacacctct gtcagaggct
     3601 gaggcagcct tcttgcaagc tgcagaggct ccagctggtg tcttgcggcc tgaccagcga
     3661 ttgttgtcag gatctggcta gcgtgctgag cgcttctcct agcctgaagg agctggacct
     3721 gcagcagaac aacctggacg acgtgggcgt gagactgctc tgcgaaggac tgagacatcc
     3781 cgcttgcaag ctgatcaggc tgggactgga ccagaccaca ctgagcgacg agatgaggca
     3841 ggagctgaga gctctggagc aggagaagcc tcagctgctg atcttcagcc ggaggaagcc
     3901 tagcgtgatg acccctacag agggcctgga taccggagag atgagcaaca gcaccagcag
     3961 cctgaagagg cagagactgg gcagcgaaag agccgcttct cacgtggccc aggccaatct
     4021 gaagctgctg gacgtgtcca agatcttccc catcgccgag atcgccgagg aatcttctcc
     4081 agaggtggtg ccagtggaac tgctctgtgt gccttctcca gccagccagg gagatctgca
     4141 cacaaagcct ctgggcaccg acgacgactt ttggggacct acaggcccag tggctacaga
     4201 agtggtggac aaggagaaga acctgtaccg ggtgcacttc cccgtggcag gatcttatcg
     4261 ctggcctaac accggcctct gcttcgtgat gagggaagcc gtgaccgtcg agatcgagtt
     4321 ttgcgtctgg gaccagttcc tcggcgagat caaccctcag cactcttgga tggtggcagg
     4381 acctctgctg gatatcaagg ccgagccagg agcagtggag gcagtgcatc tgccacactt
     4441 cgtggctctg cagggaggac acgtggatac aagcctgttc cagatggccc acttcaagga
     4501 ggagggcatg ctgctggaga agccagctag agtggagctg caccacatcg tgctggagaa
     4561 ccctagcttt tcccctctgg gcgtgctgct gaagatgatc cacaacgccc tgcggttcat
     4621 cccagtgaca agcgtcgtgc tgctgtacca cagggtgcat ccagaggagg tcaccttcca
     4681 cctgtacctg atccccagcg attgcagcat ccggaaggcc atcgacgacc tggagatgaa
     4741 gttccagttc gtgcggatcc acaagcctcc tcctctgaca cccctgtaca tgggttgccg
     4801 gtacacagtg tccggctcag gaagcggaat gctggagatc ctgcccaagg agctcgagct
     4861 ctgttacaga agcccaggcg aggaccagct gttcagcgag ttttacgtgg gccacctggg
     4921 aagcggaatc cggctgcagg tcaaggacaa gaaggacgag acactcgtct gggaagctct
     4981 ggtgaagcca ggcgatctga tgccagccac aaccctgatc cctccagcca gaattgccgt
     5041 gccttctcct ctggacgctc ctcagctgct gcatttcgtg gaccagtacc gggagcagct
     5101 gatcgctaga gtgacctccg tggaagtggt gctggacaag ctgcacggac aggtgctgag
     5161 tcaggagcag tacgagaggg tgctggcaga gaacaccagg cctagccaga tgcggaagct
     5221 gttcagcctg agccagtctt gggacaggaa gtgcaaggac ggcctgtacc aggctctgaa
     5281 ggagacacac ccccacctga tcatggagct ctgggagaag ggaagcaaga agggcctgct
     5341 gcctctgtct agccaccacc accaccacca ctagggatcc actagtccag tgtggtggaa
     5401 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc
     5461 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5521 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5581 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5641 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     5701 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5761 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5821 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5881 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5941 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6001 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6061 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6121 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     6181 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6241 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6301 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6361 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6421 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6481 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6541 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     6601 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6661 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6721 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6781 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6841 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6901 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6961 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     7021 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     7081 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     7141 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     7201 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7261 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7321 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7381 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     7441 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7501 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7561 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7621 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7681 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7741 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7801 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7861 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7921 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8521 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9481 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9841 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
