LOCUS       sequence-328405-        9852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression and purification of NLRP1 (without the PYD and CARD) from
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG
            cod...
FEATURES             Location/Qualifiers
     source          1..9852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2804..2815
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4607..4627
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(4646..4648,4649..5359)
                     /codon_start=1
                     /product="enhanced GFP (EGFP) derivative that exhibits 
                     robust folding and enhanced photostability (Zhong et al., 
                     2019)"
                     /label=SiriusGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4688..4709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     5296..5317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5360..5383
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5405..5423)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5406..5423)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5443..5460)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5449..5673
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5719..6147
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5806..5825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6016..6037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6156..6176)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6161..6490
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6341..6476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6403..6422
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6557..7351
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6611..6630)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7221..7240
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7525..7646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7562..7581)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7616..7635
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7695..7711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7695..7711)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7708..7730)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7719..7735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7743..7749,7750..7767,7768..7773))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7788..7809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7926..7943)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8097..8685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8177..8196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8856..9647,9648..9716))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9479..9498
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9717..9821)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccctacttcc aatccaatgc caccatgtgg agagaaatct cagccagcct
      961 gctgtatcag gccctgcctt ctagcccaga tcacgagagc cctagccagg agtctcctaa
     1021 cgcccctaca agcaccgcag tgctgggatc ttggggatct cctccacagc catctctggc
     1081 tcctagagag caggaggctc caggaactca gtggcctctg gacgaaacca gcggcatcta
     1141 ctacaccgag atccgggaga gagagaggga gaagagcgag aagggcagac ctccttgggc
     1201 agcagtcgtg ggaacacctc ctcaggctca cacatctctg cagcctcacc accacccttg
     1261 ggaacctagc gtgagagaga gcctctgcag cacttggcct tggaagaacg aggacttcaa
     1321 ccagaagttc acccagctgc tgctgctgca gagacctcat cctaggagcc aggatcctct
     1381 ggtgaagcgg tcttggccag actacgtgga ggagaacaga ggccacctga tcgagatccg
     1441 ggacctgttt ggcccaggac tggataccca ggagcccaga atcgtgatcc tgcagggagc
     1501 cgccggaatt ggcaaaagca cactggccag acaggtcaag gaggcttggg gaagaggaca
     1561 gctgtacggc gacagattcc agcacgtgtt ctacttctct tgccgggagc tggcccagtc
     1621 taaagtggtg tccctggccg agctgatcgg aaaagacgga acagccaccc cagcccctat
     1681 cagacagatc ctgagcaggc cagagaggct gctgttcatc ctggacggag tcgacgaacc
     1741 aggttgggtg ctgcaggaac caagcagcga gctctgcctg cattggtctc agcctcagcc
     1801 agcagacgct ctcctgggaa gcctgctggg caagacaatc ctgccagagg ccagcttcct
     1861 gatcaccgct agaaccaccg ccctgcagaa cctgatccct tctctggaac aggctcgctg
     1921 ggtggaagtg ctgggattca gcgagagcag ccggaaggag tacttctacc gctacttcac
     1981 cgacgagagg caggctatca gggccttccg gctggtgaag agcaacaagg agctgtgggc
     2041 tctgtgtctg gtgccttggg tctcttggct ggcttgcact tgcctgatgc agcagatgaa
     2101 gcggaaggag aagctgaccc tgaccagcaa gacaaccacc accctgtgcc tgcactatct
     2161 ggctcaggct ctgcaggctc agcctctggg acctcagctg agagatctgt gttctctggc
     2221 cgccgaaggc atttggcaga agaagaccct gttcagcccc gacgacctga gaaagcacgg
     2281 actggacgga gccatcatca gcaccttcct gaagatgggc atcctgcagg agcaccctat
     2341 ccctctgagc tacagcttca tccacctctg cttccaggag ttcttcgccg ccatgagcta
     2401 cgtgctggaa gacgaaaagg gccggggcaa gcacagcaat tgcatcatcg acctggagaa
     2461 gaccctggag gcctacggaa tccacggact gtttggcgcc agcacaacca gattcctgct
     2521 gggcctgctg tcagacgagg gagagcgcga gatggagaac atcttccatt gcaggctgag
     2581 ccagggcaga aacctgatgc agtgggtgcc atctctgcag ctgctcctgc agcctcatag
     2641 cctggagagc ctgcattgcc tgtacgagac ccggaacaag accttcctga cccaggtcat
     2701 ggcccacttc gaggagatgg gcatgtgcgt ggagaccgac atggagctcc tcgtctgcac
     2761 cttttgcatc aagttcagcc ggcacgtgaa gaagctgcag ctgatcgagg gcagacagca
     2821 cagaagcact tggagcccta ccatggtggt gctctttcgg tgggtcccag tgaccgacgc
     2881 ctattggcag atcctgttca gcgtgctgaa ggtcacccgg aacctgaagg agctggacct
     2941 gagcggcaac tctctgtctc acagcgccgt gaagagcctg tgcaaaaccc tgaggagacc
     3001 tcgctgtctg ctggaaaccc tgagactggc aggttgcgga ctgacagcag aggattgcaa
     3061 ggacctggcc tttggcctga gggcaaatca gaccctgacc gagctggacc tgagcttcaa
     3121 cgtgctgacc gacgcaggag ccaaacacct ctgtcagagg ctgaggcagc cttcttgcaa
     3181 gctgcagagg ctccagctgg tgtcttgcgg cctgaccagc gattgttgtc aggatctggc
     3241 tagcgtgctg agcgcttctc ctagcctgaa ggagctggac ctgcagcaga acaacctgga
     3301 cgacgtgggc gtgagactgc tctgcgaagg actgagacat cccgcttgca agctgatcag
     3361 gctgggactg gaccagacca cactgagcga cgagatgagg caggagctga gagctctgga
     3421 gcaggagaag cctcagctgc tgatcttcag ccggaggaag cctagcgtga tgacccctac
     3481 agagggcctg gataccggag agatgagcaa cagcaccagc agcctgaaga ggcagagact
     3541 gggcagcgaa agagccgctt ctcacgtggc ccaggccaat ctgaagctgc tggacgtgtc
     3601 caagatcttc cccatcgccg agatcgccga ggaatcttct ccagaggtgg tgccagtgga
     3661 actgctctgt gtgccttctc cagccagcca gggagatctg cacacaaagc ctctgggcac
     3721 cgacgacgac ttttggggac ctacaggccc agtggctaca gaagtggtgg acaaggagaa
     3781 gaacctgtac cgggtgcact tccccgtggc aggatcttat cgctggccta acaccggcct
     3841 ctgcttcgtg atgagggaag ccgtgaccgt cgagatcgag ttttgcgtct gggaccagtt
     3901 cctcggcgag atcaaccctc agcactcttg gatggtggca ggacctctgc tggatatcaa
     3961 ggccgagcca ggagcagtgg aggcagtgca tctgccacac ttcgtggctc tgcagggagg
     4021 acacgtggat acaagcctgt tccagatggc ccacttcaag gaggagggca tgctgctgga
     4081 gaagccagct agagtggagc tgcaccacat cgtgctggag aaccctagct tttcccctct
     4141 gggcgtgctg ctgaagatga tccacaacgc cctgcggttc atcccagtga caagcgtcgt
     4201 gctgctgtac cacagggtgc atccagagga ggtcaccttc cacctgtacc tgatccccag
     4261 cgattgcagc atccggaagg ccatcgacga cctggagatg aagttccagt tcgtgcggat
     4321 ccacaagcct cctcctctga cacccctgta catgggttgc cggtacacag tgtccggctc
     4381 aggaagcgga atgctggaga tcctgcccaa ggagctcgag ctctgttaca gaagcccagg
     4441 cgaggaccag ctgttcagcg agttttacgt gggccacctg ggaagcggaa tccggctgca
     4501 ggtcaaggac aagaaggacg agacactcgt ctgggaagct ctggtgaagc caggcgatct
     4561 gatgccagcc acaaccctga tccctccagg cattggtagt gggagcgaaa acctttactt
     4621 ccagggcaac ggcagcagcg gatccgtgag caagggcgag gagctgttca ccggggtggt
     4681 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgcgcggcga
     4741 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     4801 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     4861 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4921 cgtccaggag cgcaccatct ccttcaagga cgacggcacc tacaagaccc gcgccgaggt
     4981 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5041 ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat
     5101 cacggccgac aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacgtcga
     5161 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5221 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     5281 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5341 catggacgag ctgtacaagg actacaagga cgatgacgac aagtagtaat ctagagggcc
     5401 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
     5461 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5521 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5581 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     5641 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     5701 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5761 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5821 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5881 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5941 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6001 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6061 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6121 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     6181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6241 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6301 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6361 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6421 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6481 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     6541 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6601 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6661 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6721 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6781 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6841 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6901 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6961 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7021 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7081 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7141 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7201 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7261 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7321 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     7381 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7441 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7501 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7561 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7621 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7681 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7741 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7801 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9181 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9241 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9301 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9361 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9421 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9481 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9541 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9601 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9661 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9721 ttcctttttc attattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9781 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9841 ccacctgacg tc
//
