LOCUS       sequence-328427-       11814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with
            N-term eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11814)
  TITLE     Alexandrov Lab Plasmids
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pmCellFree_KA1_SARS-COV-2_NSP3
FEATURES             Location/Qualifiers
     source          1..11814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     184..1011
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             184..308
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(346..951)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1143..1446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1452..1729
                     /label=chicken beta-actin promoter
     primer_bind     2008..2030
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2092..2111
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2150..2169
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2150..2168
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2312..2335
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             2342..3052
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             2342..3052
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(2381..2402)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2642..2661)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2989..3010
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3053..3076
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    3077..3101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8943..8967)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9002..9019
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9109..9128)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9174..9229
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9228..9247)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      9317..9364
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     9377..10082
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           9465..9837
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(9494..10082)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(10091..10124)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(10292..10879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10371..10390)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10892..11683,11684..11752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11515..11534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctctcctgca tcttttaatc aaatcccaag atgtgtataa acgcgccggt atgtacagga
       61 agaggtttat actaaactgt tacattgcaa acgtggtttc gtgtgccaag tgtgaaaacc
      121 gatgtttaat caaggctctg acgcatttct acaaccacga ctccaagtgt gtgggtgaag
      181 tcatgcatct tttaatcaaa tcccaagatg tgtataaacc accaaactgc caaaaaatga
      241 aaactgtcga caagctctgt ccgtttgctg gcaactgcaa gggtctcaat cctatttgta
      301 attattgaat aataaaacaa ttataaatgt caaatttgtt ttttattaac gatacaaacc
      361 aaacgcaaca agaacatttg tagtattatc tataattgaa aacgcgtagt tataatcgct
      421 gaggtaatat ttaaaatcat tttcaaatga ttcacagtta atttgcgaca atataatttt
      481 attttcacat aaactagacg ccttgtcgtc ttcttcttcg tattccttct ctttttcatt
      541 tttctcttca taaaaattaa catagttatt atcgtatcca tatatgtatc tatcgtatag
      601 agtaaatttt ttgttgtcat aaatatatat gtctttttta atggggtgta tagtaccgct
      661 gcgcatagtt tttctgtaat ttacaacagt gctattttct ggtagttctt cggagtgtgt
      721 tgctttaatt attaaattta tataatcaat gaatttggga tcgtcggttt tgtacaatat
      781 gttgccggca tagtacgcag cttcttctag ttcaattaca ccatttttta gcagcaccgg
      841 attaacataa ctttccaaaa tgttgtacga accgttaaac aaaaacagtt cacctccctt
      901 ttctatacta ttgtctgcga gcagttgttt gttgttaaaa ataacagcca ttgtaatgag
      961 acgcacaaac taatatcaca aactggaaat gtctatcaat atatagttgc tgatggccgg
     1021 ccgtaatgag acgcacaaac taatatcaca aactggaaat gtctatcaat atatagttgc
     1081 tctagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     1141 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1201 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1261 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     1321 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     1381 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     1441 accatgcatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1501 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1561 gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag
     1621 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     1681 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     1741 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     1801 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctt cgggctgtaa
     1861 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
     1921 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
     1981 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2041 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2101 tggttattgt gctgtctcat cattttggca aagaattgga tcggacgtct aatacgactc
     2161 actataggga catcttaagt ttattttatt ttattttatt ttattttatt ttattttatt
     2221 ttattttatt ttattttatt taaccatgac agtaatgtat aaagtctgta aagacattaa
     2281 acacgtaagt gaaaccatgg gcacctctgg tcaccaccat caccaccatc accacggcac
     2341 aagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2401 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2461 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2521 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2581 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2641 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2701 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2761 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     2821 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2881 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2941 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3001 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctataca agctggaggt
     3061 gctcttccag ggtccgacaa gtttgtacaa aaaagttggc atggccccta ccaaggtgac
     3121 cttcggagac gacaccgtga tcgaggtgca gggttacaag agcgtgaaca tcaccttcga
     3181 gctggacgaa cgcatcgaca aggtgctgaa cgaaaagtgc tccgcttaca ccgtggagct
     3241 gggcaccgag gtgaacgaat tcgcctgcgt ggtggccgac gctgtgatca agaccctgca
     3301 gccagtgtcc gaactgctga cccctctggg tatcgacctg gacgagtggt ctatggctac
     3361 ctactacctg ttcgacgaga gcggcgaatt caagctggcc tctcacatgt actgcagctt
     3421 ctaccctccc gacgaagacg aggaagaggg tgactgcgaa gaggaagagt tcgagccttc
     3481 cactcagtac gagtacggca ccgaagacga ctaccagggc aagcctctgg aattcggagc
     3541 cacctctgcc gctctgcagc ctgaagagga acaggaggaa gactggctgg acgacgactc
     3601 ccagcagacc gtgggtcagc aggacggctc tgaagacaac cagaccacca ccatccagac
     3661 catcgtggag gtgcagcctc agctggagat ggaactgacc ccagtggtgc agaccatcga
     3721 ggtgaacagc ttctccggct acctgaagct gaccgacaac gtgtacatca agaacgctga
     3781 catcgtggag gaagccaaga aggtgaaacc aaccgtggtg gtgaacgccg ctaacgtgta
     3841 cctgaagcac ggcggaggtg tggctggagc tctgaacaag gctaccaaca acgccatgca
     3901 ggtggaatcc gacgactaca tcgctaccaa cggtcctctg aaggtgggcg gatcctgcgt
     3961 gctgtctggc cacaacctgg ccaagcactg cctgcacgtg gtgggaccaa acgtgaacaa
     4021 gggagaggac atccagctgc tgaagagcgc ctacgaaaac ttcaaccagc acgaggtgct
     4081 gctggctcca ctgctgtctg ccggaatctt cggtgctgat cccatccaca gcctgcgcgt
     4141 gtgcgtggac accgtgagga ccaacgtgta cctggccgtg ttcgacaaga acctgtacga
     4201 caagctggtg agctccttcc tggagatgaa gtccgaaaag caggtggagc agaagatcgc
     4261 cgagatcccc aaggaggaag tgaagccttt catcaccgaa tctaagccca gcgtggagca
     4321 gagaaagcag gacgacaaga agatcaaggc ctgcgtggag gaagtgacca ccaccctgga
     4381 ggaaaccaag ttcctgaccg agaacctgct gctgtacatc gacatcaacg gcaacctgca
     4441 ccctgactcc gctaccctgg tgtctgacat cgacatcacc ttcctgaaga aggacgcccc
     4501 ctacatcgtg ggcgacgtgg tgcaggaagg agtgctgacc gctgtggtca tccctaccaa
     4561 gaaggccggt ggcaccaccg agatgctggc caaggctctg cgtaaggtgc caaccgacaa
     4621 ctacatcacc acctaccccg gtcagggcct gaacggttac accgtggagg aagctaagac
     4681 cgtgctgaag aagtgcaagt ctgccttcta catcctgccc tccatcatct ctaacgagaa
     4741 gcaggaaatc ctgggcaccg tgagctggaa cctgcgcgaa atgctggccc acgctgagga
     4801 aaccaggaag ctgatgcccg tgtgcgtgga gaccaaggcc atcgtgagca ccatccagag
     4861 aaagtacaag ggcatcaaga tccaggaggg tgtggtggac tacggcgctc gtttctactt
     4921 ctacacctcc aagaccaccg tggcctctct gatcaacacc ctgaacgacc tgaacgagac
     4981 cctggtgacc atgccactgg gatacgtgac ccacggtctg aacctggagg aagccgctag
     5041 atacatgcgt tccctgaagg tgcctgctac cgtgagcgtg agcagccctg acgctgtgac
     5101 cgcctacaac ggatacctga cctcctctag caagacccct gaggaacact tcatcgaaac
     5161 catcagcctg gccggttcct acaaggactg gtcttacagc ggacagtcca cccagctggg
     5221 catcgagttc ctgaagcgcg gagacaagag cgtgtactac acctccaacc caaccacctt
     5281 ccacctggac ggtgaagtga tcaccttcga caacctgaag accctgctgt ctctgcgcga
     5341 ggtgaggacc atcaaggtgt tcaccaccgt ggacaacatc aacctgcaca cccaggtggt
     5401 ggacatgagc atgacctacg gacagcagtt cggtcctacc tacctggacg gagctgacgt
     5461 gaccaagatc aagccacaca actcccacga gggcaagacc ttctacgtgc tgcctaacga
     5521 tgacaccctg agagtggagg ctttcgaata ctaccacacc accgacccat ccttcctggg
     5581 caggtacatg tctgccctga accacaccaa gaagtggaag tacccccagg tgaacggact
     5641 gaccagcatc aagtgggccg acaacaactg ctacctggcc accgctctgc tgaccctgca
     5701 gcagatcgaa ctgaagttca acccacccgc tctgcaggac gcttactaca gagcccgtgc
     5761 tggcgaggcc gctaacttct gcgctctgat cctggcctac tgcaacaaga ccgtgggcga
     5821 actgggagac gtgagagaga ccatgagcta cctgttccag cacgccaacc tggactcctg
     5881 caagcgtgtg ctgaacgtgg tgtgcaagac ctgcggccag cagcagacca ccctgaaggg
     5941 agtggaagct gtgatgtaca tgggtaccct gtcctacgag cagttcaaga agggcgtgca
     6001 gatcccttgc acctgcggaa agcaggccac caagtacctg gtgcagcagg aaagcccatt
     6061 cgtgatgatg tccgctcctc cagcccagta cgaactgaag cacggcacct tcacctgcgc
     6121 ttccgagtac accggcaact accagtgcgg acactacaag cacatcacct ctaaggagac
     6181 cctgtactgc atcgacggag ccctgctgac caagtcctct gaatacaagg gtcccatcac
     6241 cgacgtgttc tacaaggaga acagctacac caccaccatc aagcccgtga cctacaagct
     6301 ggacggcgtg gtgtgcaccg aaatcgaccc taagctggac aactactaca agaaggacaa
     6361 ctcttacttc accgagcagc ctatcgacct ggtgccaaac cagccctacc ctaacgccag
     6421 cttcgacaac ttcaagttcg tgtgcgacaa catcaagttc gctgacgacc tgaaccagct
     6481 gaccggatac aagaagcctg cctctagaga gctgaaggtg accttcttcc cagacctgaa
     6541 cggcgacgtg gtggctatcg actacaagca ctacacccca agcttcaaga agggagccaa
     6601 gctgctgcac aagcccatcg tgtggcacgt gaacaacgct accaacaagg ccacctacaa
     6661 gcctaacacc tggtgcatcc gttgcctgtg gtctaccaag ccagtggaaa cctccaactc
     6721 tttcgacgtg ctgaagagcg aggacgctca gggcatggac aacctggcct gcgaagacct
     6781 gaagcccgtg agcgaggaag tggtggagaa cccaaccatc cagaaggacg tgctggaatg
     6841 caacgtgaag accaccgagg tggtgggaga catcatcctg aagccagcta acaacagcct
     6901 gaagatcacc gaggaagtgg gtcacaccga cctgatggcc gcttacgtgg acaacagctc
     6961 cctgaccatc aagaagccca acgagctgag cagggtgctg ggtctgaaga ccctggctac
     7021 tcacggtctg gctgctgtga actctgtgcc atgggacacc atcgctaact acgccaagcc
     7081 tttcctgaac aaggtggtgt ccaccaccac caacatcgtg accagatgtc tgaaccgcgt
     7141 gtgcaccaac tacatgccct acttcttcac cctgctgctg cagctgtgca ccttcaccag
     7201 aagcaccaac tcccgtatca aggctagcat gcctaccacc atcgccaaga acaccgtgaa
     7261 gagcgtggga aagttctgcc tggaggcttc cttcaactac ctgaagtctc caaacttcag
     7321 caagctgatc aacatcatca tctggttcct gctgctctcc gtgtgcctgg gttccctgat
     7381 ctactctacc gccgctctgg gagtgctgat gtctaacctg ggtatgccta gctactgcac
     7441 tggttacaga gagggatacc tgaacagcac caacgtgacc atcgccacct actgcaccgg
     7501 cagcatccca tgctccgtgt gcctgtctgg actggacagc ctggacacct acccctccct
     7561 ggaaaccatc cagatcacca tctctagctt caagtgggac ctgaccgcct tcggtctggt
     7621 ggctgagtgg ttcctggcct acatcctgtt caccaggttc ttctacgtgc tgggcctggc
     7681 cgctatcatg cagctgttct tctcctactt cgctgtgcac ttcatcagca actcctggct
     7741 gatgtggctg atcatcaacc tggtgcagat ggctcccatc tctgccatgg tgcgcatgta
     7801 catcttcttc gccagcttct actacgtgtg gaagtcctac gtgcacgtgg tggacggttg
     7861 caactcctct acctgcatga tgtgctacaa gcgcaacagg gctaccaggg tggagtgcac
     7921 caccatcgtg aacggcgtgc gcaggtcctt ctacgtgtac gccaacggcg gcaagggatt
     7981 ctgcaagctg cacaactgga actgcgtgaa ctgcgacacc ttctgcgctg gctccacctt
     8041 catctctgac gaggtggcca gagacctgag cctgcagttc aagcgtccaa tcaaccccac
     8101 cgaccagagc tcctacatcg tggactctgt gaccgtgaag aacggtagca tccacctgta
     8161 cttcgacaag gctggccaga agacctacga gcgccactct ctgagccact tcgtgaacct
     8221 ggacaacctg agggccaaca acaccaaggg cagcctgcct atcaacgtga tcgtgttcga
     8281 cggaaagtcc aagtgcgagg aatctagcgc caagtccgcc agcgtgtact acagccagct
     8341 gatgtgccag ccaatcctgc tgctggacca ggctctggtg tctgacgtgg gagacagcgc
     8401 cgaagtggcc gtgaagatgt tcgacgccta cgtgaacacc ttctcctcta ccttcaacgt
     8461 gcctatggag aagctgaaga ccctggtggc tactgctgag gctgaactgg ctaagaacgt
     8521 gtccctggac aacgtgctga gcaccttcat ctccgccgct aggcagggtt tcgtggactc
     8581 tgacgtggaa accaaagacg tggtggagtg cctgaagctg tctcaccaga gcgacatcga
     8641 agtgaccggc gactcctgca acaactacat gctgacctac aacaaggtgg agaacatgac
     8701 cccaagagac ctgggagctt gcatcgactg ctccgcccgt cacatcaacg ctcaggtggc
     8761 caagtctcac aacatcgccc tgatctggaa cgtgaaggac ttcatgagcc tgtccgagca
     8821 gctgcgcaag cagatcagga gcgccgctaa gaagaacaac ctgcccttca agctgacctg
     8881 cgctaccacc agacaggtgg tgaacgtggt gaccaccaag atcgccctga agggcggata
     8941 atacccaact ttcttgtaca aagtggtgta agcggccgcc agctttctag accagtttaa
     9001 acatcaccat caccatcact aagtgattaa cctcaggtgc aggctgccta tcagaaggtg
     9061 gtggctggtg tggccaatgc cctggctcac aaataccact gagatcgatc tttttccctc
     9121 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     9181 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     9241 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     9301 atatgcccat atgtaactag cataacccct tggggcctct aaacgggtct tgaggggttt
     9361 tttgctgaaa gcatgcggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg
     9421 agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt
     9481 attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta
     9541 cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca
     9601 aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa
     9661 ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta
     9721 atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt
     9781 cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg
     9841 cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa
     9901 atagagcttg gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt
     9961 cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac
    10021 gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa
    10081 ttgcgatcgc ataacttcgt atagcataca ttatacgaag ttataagctc ggaacgctgc
    10141 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10201 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10261 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc ctgacgagca
    10321 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10381 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10441 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
    10501 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10561 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10621 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10681 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10741 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10801 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10861 cagaaaaaaa ggatctcaag aagatccttt gttaccaatg cttaatcagt gaggcaccta
    10921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11761 tttttcaata ttattgaagc atttatcagg gttattgtct catgtccgcg cgtt
//
