LOCUS       sequence-32853-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Mouw KW, Rice PA
  TITLE     Shaping the Borrelia burgdorferi genome: crystal structure and 
            binding properties of the DNA-bending protein Hbb.
  JOURNAL   Mol Microbiol. 2007 Mar . 63(5):1319-30.
  PUBMED    17244195
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2007 Mar . 63(5):1319-30."
COMMENT     Sequence Label: pET21a Hbb
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             375..392
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(445..463)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      459..506
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(630..649)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     840..861
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatataca tatgtctttt tcacgccgcc
       61 caaaggttac taagtcagac attgttgatc aaatagcttt gaatattaaa aataataatc
      121 tgaaattaga aaaaaaatac ataagacttg taatagatgc tttttttgaa gagcttaaaa
      181 gtaatctttg ttcgaataat gttattgagt ttagatcttt tggtacattt gaagttagaa
      241 aaagaaaggg acgcttaaat gctcgaaatc ctcaaacagg ggagtatgtt aaggtcctag
      301 atcatcacgt tgcgtatttt cgtccaggca aagatttgaa agagcgcgtg tggggtatca
      361 aaggttaact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      421 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      481 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      541 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      601 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      661 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      721 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      781 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      841 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatc ttg
//
