LOCUS       sequence-328533-       12911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blasticidin resistant FC31 integrase mediated RMCE cassette for 
            upgrading MiMic insertions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12911)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 12911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31
FEATURES             Location/Qualifiers
     source          1..12911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1579..2030)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    3086..3155
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(3204..3659)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3341..3362)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3553..3572
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(3772..4431)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4346..4365
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4432..4534)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      4649..5237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5138..5157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5366..5428
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(6260..8077)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     protein_bind    6293..6304
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    10358..10427
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    10620..10754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10657..10676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10711..10730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 attcacaact gatgctctca gccaccccgc gcccttttat accgctgcgc tcgatccccg
       61 ggcgagctcg ggaggggcat gacgtaccta cctcgactgc tactcgactt aaaggcattt
      121 caagggtttc cactgggctc gacttaaagg catttcaagg gtttccactg ggctcgactt
      181 aaaggcattt caagggtttc cactgggctc gacttaaagg catttcaagg gtttccactg
      241 ggctcgactt aaaggcattt caagggtttc cactgggctc gacagtcagg tgctagtcgt
      301 acgctcctga cagcggaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt
      361 gccaagctta cacgcagtag tagcgatcgc cgaaatcgcc caggccgggc accagatatt
      421 tgttctcatc caatccgcga tccaaggctc cggtcacgat gcggacctcc gggaaggcgg
      481 cgtggtactt ctcaatgccc tccttgctgc aaatcaggtt cagaaagtag atgcgctcgg
      541 gcttcacgcc gcgcttgatc agcacctcgg tggccatgat ggcgctgccg ccggtggcca
      601 gcatgggatc gagcaggaac acgtagcgct cggaaatatc ctcgggcagc ttctcgtaaa
      661 acagcttggg cagggcggtt tcctcgtcgc gctggatcag gatcttgccg atgcgcacgc
      721 tgcggcagca atcgcgcagg ccctgctcca tgctctcgcc ggcacgcacg atgctcacgc
      781 cgcagatctt gcccatgaag ctaacgccct cgaagttctc gttggtatcg gtttcgacga
      841 tctgcttctg cacgggcagg tggttcaggc cctcctcgac cagcaggcgg atgatgcggt
      901 cacaatagaa gatgaaatct gggcgggtgg tgttcttgtt gcggatgatg gtgtacaggc
      961 ccagcagttg gttggtctgg ggcagcagat acacgttctt gaagggctcg ctggcctcgc
     1021 caatatcctc gaaccaatcc tgggggcgct catcgatgaa ctgcttcatg atctttttgc
     1081 atcgctcatc atccaccacc accacctcgt gtccgcgggt ttgcagatac ttctcgccct
     1141 tgctcttgaa gttcacgttc tcgcccacca cgcagcgggg gatgccgtac atgatgatgg
     1201 cgccggtgca catatcgcag gggctcaggg tggtgtacag ggtggtatcc ttgtacacct
     1261 tgccctccaa gcgtccgcag ttctccaggg tgctgatctc gccgtgcaag gtggcgctgc
     1321 ccttctggaa gcgcatgttg tggccgcgtc ccagcacgct gccatccttg ttgttgatca
     1381 agcagccgcc aatggggacg ccgccctcct tgtagcccaa ggcggcctcc tcataggcaa
     1441 tatccatgcc cttttgatcc cacttgctgg ccatgccgcc ggtcaccatt ttgttttcct
     1501 ccttatgtta agcttactca gttattatat cataaatatc tgtgtcaaga ataaactccc
     1561 acatggattc gagtattgag agttctcttc ttgtattcaa taattacttc ttggcagatt
     1621 tcagtagttg cagttgattt acttggttgc tggttacttt taattgattc actttaactt
     1681 gcactttact gcagattgtt tagcttgttc agctgcgctt gtttatttgc ttagctttcg
     1741 cttagcgacg tgttcacttt gcttgtttga attgaattgt cgctccgtag acgaagcgcc
     1801 tctatttata ctccggcgct cttttcgcga acattcgagg cgcgctctct cgaaccaacg
     1861 agagcagtat gccgtttact gtgtgacaga gtgagagagc attagtgcag agagggagcg
     1921 acccaaaaag aaaagagaga ataacgaata acggccagag aaatttctcg agttttcttt
     1981 ctgccaaaca aatgacctac cacaataacc agtttgtttt gggattctag ctcctgacag
     2041 cggaacaaac gacccaacac ccgtgcgttt tattctgtct ttttattgcc aagcttagtt
     2101 acgggtatac ttcaggggaa tcagttcctc aatggtggtc ttgaccagct tgccgttcat
     2161 ctcaatcagc acgaagcaat cgggggcgta atcgctaatc agctcacggc acattccaca
     2221 cggggacacg acacggatgc tacggtccac ctcatcggag tatgggtgac gcacggccac
     2281 aatggtatca aaatccttct ggccgttgct cacggcactg ccaatggcaa tggcctcggc
     2341 acacacggtc acgcgtccga tgtaggcctc aatgtgcacg gcactgataa tctcgcccgt
     2401 cttggtgcga atggcagctc ccacgtggtg cttgttatcc tcgtagagca tcgtaatctt
     2461 ctcggtagcc acctccacca gttccaggtc ctgctggctg atgttgaagg ttttcatttt
     2521 gttttcctcc ttatgttaag cttactcagt tattatatca taaatatctg tgtcaagaat
     2581 aaactcccac atggattcga gtattgagtg ttctcttctt gtattcaata attacttctt
     2641 ggcagatttc agtagttgca gttgatttac ttggttgctg gttactttta attgattcac
     2701 tttaacttgc actttactgc agattgttta gcttgttcag ctgcgcttgt ttatttgctt
     2761 agctttcgct tagcgacgtg ttcactttgc ttgtttgaat tgaattgtcg ctccgtagac
     2821 gaagcgcctc tatttatact ccggcgctct tttcgcgaac attcgaggcg cgctctctcg
     2881 aaccaacgag agcagtatgc cgtttactgt gtgacagagt gagagagcat tagtgcagag
     2941 agggagcgac ccaaaaagaa aagagagaat aacgaataac ggccagagaa atttctcgag
     3001 ttttctttct gccaaacaaa tgacctacca caataaccag tttgttttgg gattctagct
     3061 cctgacagcg catcatgatg gaccagatgg gtgaggtgga gtacgcgccc ggggagccca
     3121 agggcacgcc ctggcacccg caccgcggct tcgagacagt gacctacatc gtcgaccaat
     3181 tcctcctgag accggatccg ttaaaattgt aagcgttaat attttgttaa aattcgcgtt
     3241 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     3301 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     3361 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgctgg
     3421 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     3481 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     3541 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     3601 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgta
     3661 acatcatgtt tgacagctta tcatcgaatt tctgccattc atccgcttat tatcacttat
     3721 tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc
     3781 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     3841 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     3901 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     3961 atcaaaactg gtgaaactca cccagggatt ggctgacacg aaaaacatat tctcaataaa
     4021 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     4081 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     4141 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     4201 cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     4261 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     4321 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     4381 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     4441 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     4501 atggtgaaag ttggaacctc ttacgtgccg atcatcacaa aaggatctag gtgaagatcc
     4561 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4621 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4681 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4741 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4801 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4861 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4921 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4981 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5041 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5101 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5161 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5221 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5281 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtgtca
     5341 ggatccggtc tcatgacagc gttaacccta gaaagataat catattgtga cgtacgttaa
     5401 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
     5461 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
     5521 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
     5581 tctataaagt aacaaaactt ttaactgaaa gtgtttgcgc gctacgaaat gccgtcagag
     5641 actgaaatgt ctttcacttt gctgcggaag ttgagaattc aacacgaaga gcagcagtgt
     5701 ggtggatttc tcagttattg attttgcttc tgcaaagaca gcaaacgcaa aagggcaaca
     5761 tttgctggtc ggcatgcttt tcgggggagg aggactgggc gcgagcaggg cgaggcatgc
     5821 attcaaaaag ccggctccaa cggagagcga gagagcgcaa gagccaagtg tacagcaagg
     5881 caaacaacaa acaacgaaac acgaaggatg agtacttaaa accaaacaca tgatagtaca
     5941 cggtattagt tgggatcaaa gtgcacgttt ctgttaagtt ttgcaaccgc tcgttattaa
     6001 aagcagttca caaaatactt aaaagaaatc gaattagagc cagaacattt tttcaaatta
     6061 tattttgtta tacatttctt tgtaaaagga aattaaaata gaaaattcga atggcttgca
     6121 tatcctaaat atgcatttta ttattatatg cgttataata catataatta atatgttatt
     6181 aatatctaac ttatttaact aattacatca attactgcat tacatttaat tatttgaaat
     6241 ctatgtccat acattaagct taggctgcta catcctctgt cccgtcctgt gcgtcatcct
     6301 catcgtcgtc ggtaggaggc ttagcccaag tgatagatgc gcgcttctcg attggtgtgc
     6361 cttgccctct gccagtggtg ctcttagtca ccacgatctt gtccacgaag aggccgacga
     6421 aaacccgttt atcgtccacg gaagctcgcc cccaccagct tttaggtccg gtgggatcag
     6481 cgtcggcatc ctcagggaac cactgatcca gggggagctt cggggcctca gcggcctcta
     6541 gctcggcgag cctctcctcg gcgccctgtt gtctaagtgt cagtgctgcc tgctgtttcc
     6601 ggaagtgctt gcgacccact ggcccatcgt aggcgcccgc tgccctatcc tcgtagagtt
     6661 cctccagtgc attgagcgca tccgctctct cagctaccag gttggcacgt tcgccagatt
     6721 tttctggagc ctctgtcagc ttcccaaatc gtctggcggc ctcccataac agagccaggg
     6781 tttcttcatc tccttcagcg tgacggatct tgttaaagat acgttctgcg acgaacttgt
     6841 ctaatgcggc catactgaca ttacaggtgc cctcgtgctg tccgggagct gagggatcga
     6901 ccactttgcg acgtctgcat ctataactat ctttgatgct ctcttcgcct ctcttggatg
     6961 tcatgacggc gccgcattcg caataaagtt tgtccatcgc ggacaagata gcctggcctc
     7021 tagatagccc tttgcctctc ccccgtccat ccagccaggc ctgaagttcg taccattctg
     7081 ccggttcgat aataggcccg caatccagtt cgactggccg cagggtaatg gggtcgcgct
     7141 ggatccggta gccctcaatc tttgtagtgg gtgtaccgtc gggcttcttt ttatatatca
     7201 cctccgcggc gaacccggca atgcggggat cccggagaat gcgcatgact gtggcagggt
     7261 cccacgcgct tgaggcagtc tttttgccta ttgtctcccc cctcgttggg actgcgtcgg
     7321 cgtccatccg cttacaaagc ccagtaatgg agcccgggtg aatggctgcc tgagatccag
     7381 gtttaaaggg cagatgcttg tgcgtcttaa tttcacgcca ccaccaacgg atcacgtcgg
     7441 gttcgaattc aaatggccca gtcagtgggg ttgtggaatg tgccagttta ttaatgacga
     7501 cgttgaccat tcggccgttt ctggtgattt cttttgtctc gctaacgagt tcgaacccgt
     7561 agggggcctt ccctcccacg tagccgccaa gctctcgctg aagattcttt gtgtccaaga
     7621 ttttcgcgct cttaaggctg ctttctttgt ggctggcgtc caggcgcata atcaaatgaa
     7681 tcaggtccat gacatttccc tgccggaaca ctccctcctg agtgctaaca atagtcaccc
     7741 cgagtgccag cagctcactt acgataggga tggcgtccat aaccttcagc cgtgagaacc
     7801 gggaaacatc gtaaactatg atcatgttca ggcggcccgc tcggcactca ttcaggattc
     7861 tctcgaactc tggcctctct gctgtcccaa aggcactagt ccctggtgcc tcgctaaagt
     7921 ggcctacgaa tctgaatctg ccgccgtccc tctccacctc cctctgcagg tcggccgcct
     7981 tatcctcgtt agctgaccgt tgcgtggcgg ggctggctgc actggagttt tcccgctccc
     8041 gggactgtct gtcgtaggca ccggcatagg tgtccatttt gatatttttt ttttaatttg
     8101 gcctgccttt caagagcaaa ttaattcttt ttcactttat gtattacctt acaaactttg
     8161 ataagttcag atcacttcgc gcaacgcaaa accgaaaagg atctgttaaa agtaccaaaa
     8221 atctagtggt ggaatttccc attgtgctat cgcaattcga tattcacaat ttcgataatt
     8281 tatatatata gccgttttcc actaacacat caaatgtgat ttagctatta atgaaattgt
     8341 aatattcaag caacctaagt gattcattca tatgtacata cttatatgta aagatggtgg
     8401 tggcattttt aacatgggga agttcttatt tatttacttt ttcgtttgtt ataggtgaat
     8461 tcctctttat ttttatacaa tataatttta ttttacgtta agcaaccaat ttaagggcta
     8521 tgaaacgtag taactaacag gagcaacgaa gataacatgg aaacatacaa aatttgtata
     8581 aaaatattga ttagataata gtttggcttg tttatgccac gtgtccaaag atatctttga
     8641 tgacgcctcc tcagtgttct cctggttgtc tggtatgtgc ctgatttaat ttgttctcaa
     8701 actgaaggtg gatgctgagg tcatgtgcga tatcaacatt tctcgcgtac cagggcgcgt
     8761 tggtgattgt tcgcaacgct tggttttgga gccgttgccc tctcactatg tcccaccgcc
     8821 gatttgaccc gcagacgtat cccgcttgtc cccaacaacc gcacttcatc ataatacatc
     8881 atctctagac cacactcaaa cgatttattt attaaaacta agtgatatat agggaattgt
     8941 tattgaaggt aattagtata attcaaaaac gcccaacacg ctgttctttc attgaaatac
     9001 agtggagccc tgcttgcaag cagtgcaagt ttaaaaaaca tcgcaatgct atgtgtgtgt
     9061 actcgcttaa aagccagatg tacattaaaa tacggtaaca ctgttgactt ctgaaaccca
     9121 aactacttcc agttgggaat tgtattagcc atgggaaggc atttcttgac aagccaccgc
     9181 ccggtaacac taaaacgcgt tagggtgttc tgtcaactcg ttctgcgaat acagaaacga
     9241 aaaaaatagt aaacaacatt tattggaggg aaaaaaagaa atagcccggc ttccgcataa
     9301 acaactgtcg cagacccgca gcaccgatta aagccgacag ccgacgccag ttaacctaac
     9361 ctctgatcaa attaagtggg ccaaaatgag cgtggaggag gaagtgtttc gaatccaaaa
     9421 gaagatgagc aagatggcca gcgacggcac agtaagtatc gggattagct aacgtgccgc
     9481 gcatcatgtc acaaatatac atatatccac cgtacaggga caggatcagg ctctggacct
     9541 gctgaaggcc ctgcaaacgc ttaacatcaa tctcgacatt ctgaccaaaa cgcgcatcgg
     9601 catgaccgta aacgagctgc gcaagagtag caaggacgac gaggtgatcg ctctggccaa
     9661 gacgctgatc aagaactgga aacgattcct cgccagcccg gcgccaacca caccgaacaa
     9721 cagctccgct aaggagggct cctccaacaa cagcagtgcc tcgaagtcca ccagtgccgc
     9781 caagtcgtcg tcgtcaatct ccggcaagga taagtccagt tcatccagct catccaagga
     9841 taaagagaag aaaggctcga catcctcgtc acagacctcg tttccctccg gcggcatgac
     9901 agatgcggtc cgcatcaaat gccgcgaaat gctggccacc gcactgaaga ttggtgaagt
     9961 gcccgaagga tgcggtgagc cggaggaaat ggccgccgaa ctggaggatg ccatttactc
    10021 cgagttcaat aacacggata tgaagtacaa gaatcgcatt aggtcacgcg tggccaatct
    10081 gaaggacccc aaaaatcctg gattgcgagg caactttatg tgcggcgccg tcactgccaa
    10141 gcagctggcc aaaatgacgc cggaggagat ggccagcgat gagatgaaga agctgcgcga
    10201 gaagttcgtc aaggaggcca tcaacgatgc ccagctggcc accgtgcagg gcaccaagac
    10261 cgatctcctc aagtgcgcca agtgcaagaa gcgcaactgc acctacaacc agctgcagct
    10321 cctgacagcg gaattggtcg acgatgtagg tcactgtctc gaagccgcgg tgcgggtgcc
    10381 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
    10441 tgctcctgac agcggttctc cttgttctcc acggtcttaa gcttagcacg agcgatcgag
    10501 agagatagag agcgagatag agcgaagctt gttaattcgt cgagcgcttg ccatactgaa
    10561 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    10621 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10681 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10741 atcatgtctg gatcaagctc actccttgcg acgagcgcgc aggcgcagag ccagataggc
    10801 cagcacgcgg aaggacacaa tcaggatggc caggcccaca taatccagtg gcaggtcggc
    10861 ggcactgaaa ttcagtgtct ccaggatgac ctttccgctg ctggggcagg tcgtgttgct
    10921 gctggtgcag gagatctcgc cgggctccac atcggcccac tgattgatca gcaggccctc
    10981 attggcgtag cgaaaccagc tcaggtagct cagccacttc aggtacacgg gcacgctgcc
    11041 ggagttcaga aagaatccgc cgaacagcag gaaggggata atcacggggg gtcccacgct
    11101 cagggccatg ctggtgctgc tggaggcgca gctgatcagg tagccgaagc tggtcgacac
    11161 gttggccacc agggtcacca gggccaggca attaaagaaa tgcagcactc cggcacgcag
    11221 gccaatcatg gggtaggcaa tggcggtaaa caccaggggc acggtcagga acagcggcag
    11281 ctcggcaatg gtctttccca ggaagtaggt atcgcagcgg tacaggcggc tgcgggcctc
    11341 gcgcatgaac acgggcagct cgctggtgaa cacgttgatg gtggcgaaca cgttctggaa
    11401 cgtcatgttc gtcagaaaca gaaaaatggc tccattgatg ttcatcactc cgacctgcgt
    11461 cagctgctgt cccaggaaga tcaggccgat cagaatggcc accatcgtgg tctggatcag
    11521 gcggaccttg accagcaggg gctccttcag cacactcagc cagctgcgcc acagcacggc
    11581 gcgaaactgc ataaaccagg tagccttata cgtatagccg ttctcgggct gctccagggg
    11641 cttctccagg ttcttcgtgg ccagcagctg ctccatgtcg cgggccacct tggagatggc
    11701 gaagttatcg cagatcttgg caatgcgatc gcggctctca atctcgcgtc ctggcaccac
    11761 ggccagcact tgcacataga agtcggcggg gttgtaattc gtggggcact gggcgcccac
    11821 atagctgaag aaatccacgg cctcgcttgg ggttcccagg aaggccacgc gtccctcggc
    11881 catcagcaga atcttatcaa acagctcgaa cagctccgag ctgggctggt ggatcgtcag
    11941 aatgaccgtc tttcccttct ggctcagctt cttcagcacc tgcaccacgg agtgggcggt
    12001 gaagctatcc aggccgctgg tcggctcgtc gcaaatcagc agggggggat cggtcagggc
    12061 ctcgctggca aaggccaggc gcttgcgctc tccgccactc aggcccttca cgcggcctgg
    12121 cacgccaata atggtgtgct ggcacttgct caggctcagc tcctggatga cctggtccac
    12181 gcgggccacg cgctgacggt atgtcaggtg gcggggcatg cggaccatgg cctgaaagat
    12241 cagatgctcg cgggctgtca ggctgccgat gaacagatcg tcctgctgca cgtaggcgca
    12301 gcgggcctgc atctccttgg catccacggg ctggccgttc agcaggcgca tgccgcttgg
    12361 ggacacctga atgccctggg ggctgcggaa ggccagggcg ttcagcagcg tggtctttcc
    12421 ggcgccacta cttcccatca cggccagcag ctcgccgggg taggccactc cgcacacatt
    12481 cttcagcagg tgcttgcgtg gggctgggat atggcgctca ttgcagaaca gtccgcgggt
    12541 gcggttcacc agctgacgcc agccactgcc tggctggttc acggcgccga agatatccat
    12601 gttatgccag gcgtacgtca gattctcggc cagctgtccg gatccgcttc cgctatcctc
    12661 tggggggctg gggggcagca gggttccgta attcttggcc tggccaaatc cctgattgat
    12721 gcacgactgg ctggcggctc cgctatcgcc gttgttcagg tgctcggcgc ttgggtgctt
    12781 ggagcctccg cggatcagca gctcctggtc ctcctgtccc atttggtttg gtatgcgtct
    12841 tgtgattcaa agttggctta ttcaaaggat attaacgaag gcagcggcac gtctgctcgg
    12901 ctcgaacatt c
//
