LOCUS       sequence-328537-        3350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Blasticidin resistance cassette in Alpha1, lacks physical 
            marker or attB site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pHSP70B-CP6-bsr
FEATURES             Location/Qualifiers
     source          1..3350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        171..273
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             274..1089
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             274..1089
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(347..366)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1203..1658
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1290..1309)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1500..1521
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2078..2099
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     rep_origin      complement(join(2818..3350,1..56))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2898..2917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
       61 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      121 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgtga tgatcggcac
      181 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
      241 ttatcgagat tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct
      301 tgctcaaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
      361 cgcgataatg tcgggcaatc aggtgcgaca atctaccgat tgtatgggaa gcccgatgcg
      421 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
      481 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
      541 actcctgatg atgcatggtt actcaccact gcgatcccag ggaaaacagc attccaggta
      601 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
      661 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgactc
      721 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
      781 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
      841 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
      901 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
      961 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     1021 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     1081 tttttctgat tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg
     1141 aataagtgat aataagcgga tgaatggcag aaattcgatg ataagctgtc aaacatgatg
     1201 ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1261 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1321 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1381 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1441 gccagcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1501 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1561 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1621 taacgcgaat tttaacaaaa tattaacgct tacaatttta acgaattcgt ctcaggagct
     1681 agaatcccaa aacaaactgg ttattgtggt aggtcatttg tttggcagaa agaaaactcg
     1741 agaaatttct ctggccgtta ttcgttattc tctcttttct ttttgggtcg ctccctctct
     1801 gcactaatgc tctctcactc tgtcacacag taaacggcat actgctctcg ttggttcgag
     1861 agagcgcgcc tcgaatgttc gcgaaaagag cgccggagta taaatagagg cgcttcgtct
     1921 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
     1981 aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga
     2041 atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga
     2101 agtaattatt gaatacaaga agagaacact caatactcga atccatgtgg gagtttattc
     2161 ttgacacaga tatttatgat ataataactg agtaagctta acataaggag gaaaacaaaa
     2221 tgaaaacctt caacatcagc cagcaggacc tggaactggt ggaggtggct accgagaaga
     2281 ttacgatgct ctacgaggat aacaagcacc acgtgggagc tgccattcgc accaagacgg
     2341 gcgagattat cagtgccgtg cacattgagg cctacatcgg acgcgtgacc gtgtgtgccg
     2401 aggccattgc cattggcagt gccgtgagca acggccagaa ggattttgat accattgtgg
     2461 ccgtgcgtca cccatactcc gatgaggtgg accgtagcat ccgtgtcgtg tccccgtgtg
     2521 gaatgtgccg tgagctgatt agcgattacg cccccgattg cttcgtgctg attgagatga
     2581 acggcaagct ggtcaagacc accattgagg aactgattcc cctgaagtat acccgtaact
     2641 aagcttggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttccgctgt
     2701 catgagacga attctgacac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2761 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2821 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2881 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2941 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3001 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3061 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3121 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3181 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3241 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3301 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
//
