LOCUS       sequence-32854-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Swinger KK, Lemberg KM, Zhang Y, Rice PA
  TITLE     Flexible DNA bending in HU-DNA cocrystal structures.
  JOURNAL   EMBO J. 2003 Jul 15. 22(14):3749-60.
  PUBMED    12853489
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2003 Jul 15. 22(14):3749-60."
COMMENT     Sequence Label: pET21a AHU
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             345..362
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(415..433)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      429..476
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      513..871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(600..619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     810..831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgaacaa aggtgaattg gttgatgccg
       61 tagctgaaaa agctagtgtt actaaaaaac aagctgatgc cgtcttaaca gctgctttgg
      121 aaacgattat tgaagctgtc tcttctggcg ataaagttac gctggtagga tttggctctt
      181 ttgaatcacg ggaacgtaaa gctcgtgaag gtcgtaaccc caaaactaac gaaaagatgg
      241 aaattccagc gacaagggtt cctgctttct ctgccggtaa gttgttcaga gaaaaagtag
      301 cacccccaaa agcataaggt accaagcttg cggccgcact cgagcaccac caccaccacc
      361 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      421 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      481 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      661 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      841 caaccctatc tcggtctatt cttttgattt atnaagg
//
