LOCUS       sequence-32855-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     Phoebe Rice plasmids
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET21a IHF
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             658..675
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(728..746)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      742..789
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgacc aagtcagaat tgatagaaag
       61 acttgccacc cagcaatcgc acattcccgc caagacggtt gaagatgcag taaaagagat
      121 gctggagcat atggcctcga ctcttgcgca gggcgagcgt attgaaatcc gcggtttcgg
      181 cagtttctct ttgcactacc gcgcaccacg taccggacgt aatccgaaga ctggcgataa
      241 agtagaactg gaaggaaaat acgttcctca ctttaaacct ggtaaagaac tgcgcgatcg
      301 cgccaatatt tacggttaat tttgtttaag actagtgaag gagatataca tatggcgctt
      361 acaaaagctg aaatgtcaga atatctgttt gataagcttg ggcttagcaa gcgggatgcc
      421 aaagaactgg ttgaactgtt tttcgaagag atccgtcgcg ctctggaaaa cggcgaacag
      481 gtgaaactct ctggttttgg taacttcgat ctgcgtgata agaatcaacg cccgggacgt
      541 aacccgaaaa cgggcgagga tattcccatt acagcacggc gcgtggtgac cttcagaccc
      601 gggcagaagt taaaaagccg ggtcgaaaac gcttcgccca aagacgagta actcgagcac
      661 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      721 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      781 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
      841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgcac
//
