LOCUS       sequence-32858-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Wrobel CN, Mutch CA, Swaminathan S, Taketo MM, Chenn A
  TITLE     Persistent expression of stabilized beta-catenin delays maturation 
            of radial glial cells into intermediate progenitors.
  JOURNAL   Dev Biol. 2007 Sep 15. 309(2):285-97.
  PUBMED    17706960
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2007 Sep 15. 309(2):285-97."
COMMENT     Sequence Label: pCAG-delta90GFP
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(57..76)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    122..177
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(176..195)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(538..554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(538..554)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(551..573)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    562..578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(586..592,593..610,611..616))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    631..652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
       61 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
      121 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
      181 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
      241 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
      301 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
      361 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
      421 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
      481 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
      541 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      601 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      661 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
      721 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc c
//
