LOCUS       sequence-32859-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  TITLE     Chenn Lab Plasmids
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PCDNA-Delta-90AEGFP
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tagcgactct ctggctactg agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttggtaccga gctcggatcc actagtaacg gccgccagtg
      121 tgctggaatt cccatcgatt ctagaccgcg gccaccatgg ctcagagggt ccgagctgcc
      181 atgttccctg agacgctaga tgagggcatg cagatcccat ccacgcagtt tgacgctgct
      241 catcccacta atgtccagcg cttggctgaa ccatcacaga tgttgaaaca tgcagttgtc
      301 aatttgatta actatcagga tgacgcggaa cttgccacac gtgcaattcc tgagctgaca
      361 aaactgctaa acgatgagga ccaggtggta gttaataaag ctgctgttat ggtccatcag
      421 ctttccaaaa aggaagcttc cagacatgcc atcatgcgct cccctcagat ggtgtctgcc
      481 attgtacgca ccatgcagaa tacaaatgat gtagagacag ctcgttgtac tgctgggact
      541 ctgcacaacc tttctcacca ccgcgagggc ttgctggcca tctttaagtc tggtggcatc
      601 ccagcgctgg tgaaaatgct tgggtcacca gtggattctg tactgttcta cgccatcacg
      661 acactgcata atctcctgct ccatcaggaa ggagctaaaa tggcagtgcg cctagctggt
      721 ggactgcaga aaatggttgc tttgctcaac aaaacaaacg tgaaattctt ggctattaca
      781 acagactgcc ttcagatctt agcttatggc aatcaagaga gcaagctcat cattctggcc
      841 agtggtggac cccaagcctt agtaaacata atgaggacct acacttatga gaagcttctg
      901 tggaccacaa gcagagtgct gaaagtgctg tctgtctgct ctacaaacaa gccggccatt
      961 gtaaaacggg g
//
