LOCUS       sequence-32867-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Xu Y, Sengupta T, Kukreja L, Minella AC
  TITLE     MicroRNA-223 regulates cyclin E activity by modulating expression of
            F-box and WD-40 domain protein 7.
  JOURNAL   J Biol Chem. 2010 Nov 5;285(45):34439-46. doi: 
            10.1074/jbc.M110.152306. Epub 2010 Sep 7.
  PUBMED    20826802
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.152306"; journalName: "J Biol Chem"; date: 
            "2010-11-5- 5"; volume: "285"; issue: "45"; pages: "34439-46"
COMMENT     Sequence Label: pFLAG-Fbw7-alpha-3'UTR
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..100
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gagctcgttt agtgaaccgt cagaattaac catggactac aaagaccatg acggtgatta
       61 taaagatcat gacatcgatt acaaggatga cgatgacaag cttatgaatc aggaactgct
      121 ctctgtgggc agcaaaagac gacgaactgg aggctctctg agaggtaacc cttcctcaag
      181 ccaggtagat gaagaacaga tgaatcgtgt ggtagaggag gaacagcaac agcaactcag
      241 acaacaagag gaggagcaca ctgcaaggaa tggtgaagtt gttggagtag aacctagacc
      301 tggaggccaa aatgattccc agcaaggaca gttggaagaa aacaataata gatttatttc
      361 ggtagatgag gactcctcag gaaaccaaga agaacaagag gaagatgaag aacatgctgg
      421 tgaacaagat gaggaggatg aggaggagga ggagatggac caggagagtg acgattttga
      481 tcagtctgat gatagtagca gagaagatga acatacacat actaacagtg tcacgaactc
      541 cagtagtatt gtggacctgc ccgttcacca actctcctcc ccattctata caaaaacaac
      601 aaaaatgaaa agaaagttgg accatggttc tgaggtccgc tctttttctt tgggaaagaa
      661 accatgcaaa gtctcagaat atacaagtac cactgggctt gtaccatgtt cagcaacacc
      721 aacaactttt ggggacctca gagcagccaa tggccaaggg caacaacgac gccgaattac
      781 atctgtccag ccacctacag gcctccagga atggctaaaa atgtttcaga gctggagtgg
      841 accagagaaa ttgcttgctt tagatgaact cattg
//
