LOCUS       sequence-328792-        5148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic transgenesis 3xP3 white CDS physical eye marker cassette in 
            Alpha2, lacks attB site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: p3XP3-WhiteA2
FEATURES             Location/Qualifiers
     source          1..5148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     394..415
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     polyA_signal    2532..2666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2556..2575)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2610..2629
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2847..3435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2927..2946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3550..3652
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3653..4468
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3653..4468
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3726..3745)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4582..5037
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4669..4688)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4879..4900
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(5143..5148,1..446)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 tcccaaaaca aactggttat tgtggtaggt catttgtttg gcagaaagaa aactcgagaa
       61 atttctctgg ccgttattcg ttattctctc ttttcttttt gggtcgctcc ctctctgcac
      121 taatgctctc tcactctgtc acacagtaaa cggcatactg ctctcgttgg ttcgagagag
      181 cgcgcctcga atgttcgcga aaagagcgcc ggagtataaa tagaggcgct tcgtctacgg
      241 agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat
      301 aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca
      361 attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg ccaagaagta
      421 attattgaat acaagaagag aactctcaaa ggaggaaaac aaaatgggac aggaggacca
      481 ggagctgctg atccgcggag gctccaagca cccaagcgcc gagcacctga acaacggcga
      541 tagcggagcc gccagccagt cgtgcatcaa tcagggattt ggccaggcca agaattacgg
      601 aaccctgctg ccccccagcc ccccagagga tagcggaagc ggatccggac agctggccga
      661 gaatctgacg tacgcctggc ataacatgga tatcttcggc gccgtgaacc agccaggcag
      721 tggctggcgt cagctggtga accgcacccg cggactgttc tgcaatgagc gccatatccc
      781 agccccacgc aagcacctgc tgaagaatgt gtgcggagtg gcctaccccg gcgagctgct
      841 ggccgtgatg ggaagtagtg gcgccggaaa gaccacgctg ctgaacgccc tggccttccg
      901 cagcccccag ggcattcagg tgtccccaag cggcatgcgc ctgctgaacg gccagcccgt
      961 ggatgccaag gagatgcagg cccgctgcgc ctacgtgcag caggacgatc tgttcatcgg
     1021 cagcctgaca gcccgcgagc atctgatctt tcaggccatg gtccgcatgc cccgccacct
     1081 gacataccgt cagcgcgtgg cccgcgtgga ccaggtcatc caggagctga gcctgagcaa
     1141 gtgccagcac accattattg gcgtgccagg ccgcgtgaag ggcctgagtg gcggagagcg
     1201 caagcgcctg gcctttgcca gcgaggccct gaccgatccc cccctgctga tttgcgacga
     1261 gccgaccagc ggcctggata gcttcaccgc ccactccgtg gtgcaggtgc tgaagaagct
     1321 gagccagaag ggaaagacgg tcattctgac gatccaccag cccagctcgg agctgttcga
     1381 gctgtttgat aagattctgc tgatggccga gggacgcgtg gccttcctgg gaaccccaag
     1441 cgaggccgtg gatttcttca gctatgtggg cgcccagtgc cccacgaatt acaaccccgc
     1501 cgacttctat gtgcaagtgc tggccgtggt gccaggacgc gagattgaga gccgcgatcg
     1561 cattgccaag atctgcgata acttcgccat ctccaaggtg gcccgcgaca tggagcagct
     1621 gctggccacg aagaacctgg agaagcccct ggagcagccc gagaacggct atacgtataa
     1681 ggctacctgg tttatgcagt ttcgcgccgt gctgtggcgc agctggctga gtgtgctgaa
     1741 ggagcccctg ctggtcaagg tccgcctgat ccagaccacg atggtggcca ttctgatcgg
     1801 cctgatcttc ctgggacagc agctgacgca ggtcggagtg atgaacatca atggagccat
     1861 ttttctgttt ctgacgaaca tgacgttcca gaacgtgttc gccaccatca acgtgttcac
     1921 cagcgagctg cccgtgttca tgcgcgaggc ccgcagccgc ctgtaccgct gcgataccta
     1981 cttcctggga aagaccattg ccgagctgcc gctgttcctg accgtgcccc tggtgtttac
     2041 cgccattgcc taccccatga ttggcctgcg tgccggagtg ctgcatttct ttaattgcct
     2101 ggccctggtg accctggtgg ccaacgtgtc gaccagcttc ggctacctga tcagctgcgc
     2161 ctccagcagc accagcatgg ccctgagcgt gggacccccc gtgattatcc ccttcctgct
     2221 gttcggcgga ttctttctga actccggcag cgtgcccgtg tacctgaagt ggctgagcta
     2281 cctgagctgg tttcgctacg ccaatgaggg cctgctgatc aatcagtggg ccgatgtgga
     2341 gcccggcgag atctcctgca ccagcagcaa cacgacctgc cccagcagcg gaaaggtcat
     2401 cctggagaca ctgaatttca gtgccgccga cctgccactg gattatgtgg gcctggccat
     2461 cctgattgtg tccttccgcg tgctggccta tctggctctg cgcctgcgcg ctcgtcgcaa
     2521 ggagtgagct tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     2581 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2641 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2701 ttcaggggga ggtgtgggag gttttttcgc ttgagacgaa gcttgacacg gttatccaca
     2761 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2821 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2881 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2941 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3001 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3061 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3481 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3541 cttttgtgat gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
     3601 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca
     3661 tattcaacgg gaaacgtctt gctcaaggcc gcgattaaat tccaacatgg atgctgattt
     3721 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctaccgatt
     3781 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     3841 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     3901 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg
     3961 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     4021 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     4081 cgatcgcgta tttcgactcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     4141 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     4201 taaacttttg ccattctcac cggattcagt agtcactcat ggtgatttct cacttgataa
     4261 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     4321 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     4381 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     4441 tcatttgatg ctcgatgagt ttttctgatt ttttttaagg cagttattgg tgcccttaaa
     4501 cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgatga
     4561 taagctgtca aacatgatgt tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4621 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4681 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4741 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4801 gtgatggttc acgtagtggg ccagcgccct gatagacggt ttttcgccct ttgacgttgg
     4861 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4921 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4981 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaattttaa
     5041 caagcttcgt ctcagtcagg agggatctaa ttcaattaga gactaattca attagagcta
     5101 attcaattag gatccaagct tatcgatttc gaaccctctc ccctagaa
//
