LOCUS       sequence-328841-       12296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses carotenoid biosynthesis gene in response to homoserine 
            lactone and doxycycline in yeast expressing LuxTA and rtTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12296)
  AUTHORS   Tominaga M, Nozaki K, Umeno D, Ishii J, Kondo A
  TITLE     Robust and flexible platform for directed evolution of yeast genetic
            switches.
  JOURNAL   Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y.
  PUBMED    33758180
REFERENCE   2  (bases 1 to 12296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-23"; volume: "12(1):1846. doi"; pages: " 
            10.1038/s41467-021-22134-y"
COMMENT     Sequence Label: pATP416-­pluxO5-­ptetO7-­crtYBI
FEATURES             Location/Qualifiers
     source          1..12296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2009..2025
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2010..2026
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2204..2391)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     2312..2336
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        4877..5543
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5413..5436
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     protein_bind    7684..7702
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7720..7738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7755..7773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7791..7809
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7827..7845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7862..7880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7898..7916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      9188..9458
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     primer_bind     complement(9523..9543)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9523..9541)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9562..9578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9562..9578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9575..9597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9586..9602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9610..9616,9617..9634,9635..9640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9655..9676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9793..9810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9964..10552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10044..10063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10723..11514,11515..11583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11346..11365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11584..11688)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    11725..12228
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     12269..12287
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
     2041 gaattcctgc agcccggggg atccactagt ggtaccgggc cccccctcga gttgtcctct
     2101 gaggacataa aatacacacc gagattcatc aactcattgc tggagttagc atatctacaa
     2161 ttgggtgaaa tggggagcga tttgcaggca tttgctcggc atgccggtag aggtgtggtc
     2221 aataagagcg acctcatgct atacctgaga aagcaacctg acctacagga aagagttact
     2281 caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat
     2341 tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg cggccggcct
     2401 tactgccctt cccatccgct catgaccaca ctcaagactt tccgtactct cctccatccg
     2461 gcaactgtcc ttctctctcc gacgtctcct ttccaaacga ctttgatctc tcggccgatc
     2521 agtaggtagc tcgcgcaagc cgctcgcatt cccgcttgaa cctcggtagg aagtcggtca
     2581 attcccttat aagaatgttt ggcaagatcc tctgcgtagg cgactaatgg aagcgagtac
     2641 gtcttccatt cgaaccggaa gctttctgag gcgtttgaag atggtaatgt ggacgaagga
     2701 gatagactga ggagtttgtc gaaatcttga ggccgaggtt ccgtccaatc agtcgggatc
     2761 gcaagctttg attcatcccg aagaccaaag aatgagagtg gtaggtaaaa tctcccttct
     2821 gttgcgtccc ctttaatgtc cctagcaatg ttcaccaact gaagggcagt tcccatctct
     2881 cggcttgcca ctaacacagc ttctctttct tctatggtgg cagggacctg acttggtgca
     2941 cttgcccaag agacatagac caatagctcg gcgactgagc ctgctacaca tagaccatag
     3001 tccagcaagt cagctgtggt ctcgataggc gtcttccgag cctggactgc ctctgtcgat
     3061 aaaggaaaga taagatcagt ggtgtatcct ctaaggagtt cgtcgagtgg gtatcgaggg
     3121 atcagccctt gcaacttagc gagcaacctg aaggcgaaat ggtattgaac gggaaccctt
     3181 tcggtaagga attgaacgag ctcggcaggc gagagcgaag gaggaggcgg gaggggatac
     3241 attcccgtgg gtcgggaagg gtgcgaagga ggaagtaaag gcgaagaaag gatcttgtca
     3301 ggttgcgaag ggtgtagcgg gggcccaaat agtagggtaa gaaaatcgga gaccatgtca
     3361 attgtggcat gcgggttgga agatacttca ggagagtcga taagatcatc agtcacccgg
     3421 cagaatgcgt atagtccaac cagcctctcc ctaacttcgc taggaaatcc agccgaggca
     3481 acaaaaaagc tccggctctt ttcctccaac aacttgactg ccagttccaa gtcacgtttt
     3541 ggctgagaag agtatggtcg gctgctaaaa aacagggaga gcacaggcgg tgtaatgagg
     3601 ggaaatgaag atggcatctt tttgttgcca taaatagttc gaccgtgtag caggtatagg
     3661 gcctgagtat gatcgcaggc agacagaccc agaacaatca ttagattcgt cagtaagaag
     3721 aacatagctt cctcaatggg tagtacacct ccaagcctcc accctacaat cttctcatcg
     3781 ttgatcgacc aagagtcttg accgacagca acataatcta cccaaatcag atacaccgtc
     3841 gggatcatga ttgctgcaat agttgacttt gctcggccac ttttccaatc gaaagcatat
     3901 tcgcctgata atgctgccaa gagcatggta ggtggggtga tgagtaagga gagtgcccgc
     3961 atgtagaagt agtgatctgt cacgagcggg tcgggcgatg ggctggggtg agcggtaaat
     4021 aggtagataa tgggcagagg gatgagcgcc ttgagcgcga gagaaagggc ggacgatcta
     4081 gtcttgggaa gcgcgagaga tgggagaagg tgcctagttg ccaagacgta gaccaagccg
     4141 gtgattacgg tttgaatgac aaagaaagcg tactcttcat atggaacatc tagaaacgtt
     4201 ccaaacacgc cttggccact ctccgctgat ggatatgtcc atgcgccatt tctgatgatc
     4261 catgagtccc atggtgtggt tgcactaaac gcaataaata cgaggatcga tattttgtag
     4321 atgtcaaatt ttgtcaaaat cggggaagtg agcagaccga gaagaccaag aattgggaga
     4381 gtatagatca gatggatctg gtaatatgcg agagccgtca tcctaggtat agttttttct
     4441 ccttgacgtt aaagtataga ggtatattaa caattttttg ttgatacttt tattacattt
     4501 gaataagaag taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg ttatgcagtt
     4561 tttgcattta tatatctgtt aatagatcaa aaatcatcgc ttcgctgatt aattacccca
     4621 gaaataaggc taaaaaacta atcgcattat catccggatc acatacgtac ctgtacgatc
     4681 ctacaggttg acctgtacga tcctacaggt tcacctgtac gatcctacag gtggatccgc
     4741 tggcgtaata gcgacctgta cgatcctaca ggtacttatg ttcgaccttg cgtaaacctg
     4801 tacgatccta caggtactta tgttcgacct tgcgtaactt aaggaataaa aaacacgctt
     4861 tttcagttcg agtttatcat tatcaatact gccatttcaa agaatacgta aataattaat
     4921 agtagtgatt ttcctaactt tatttagtca aaaaattagc cttttaattc tgctgtaacc
     4981 cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcgta ggtgtctggg
     5041 tgaacagttt attcctggca tccactaaat ataatggagc ccgcttttta agctggcatc
     5101 cagaaaaaaa aagaatccca gcaccaaaat attgttttct tcaccaacca tcagttcata
     5161 ggtccattct cttagcgcaa ctacagagaa caggggcaca aacaggcaaa aaacgggcac
     5221 aacctcaatg gagtgatgca acctgcctgg agtaaatgat gacacaaggc aattgaccca
     5281 cgcatgtatc tatctcattt tcttacacct tctattacct tctgctctct ctgatttgga
     5341 aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac ttgactaata
     5401 agtatataaa gacggtaggt attgattgta attctgtaaa tctatttctt aaacttctta
     5461 aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac ttagtttcga
     5521 ataaacacac ataaacaaac aaaacgcgta tgggaaaaga acaagatcag gataaaccca
     5581 cagctatcat cgtgggatgt ggtatcggtg gaatcgccac tgccgctcgt cttgctaaag
     5641 aaggtttcca ggtcacggtg ttcgagaaga acgactactc cggaggtcga tgctctttaa
     5701 tcgagcgaga tggttatcga ttcgatcagg ggcccagttt gctgctcttg ccagatctct
     5761 tcaagcagac attcgaagat ttgggagaga agatggaaga ttgggtcgat ctcatcaagt
     5821 gtgaacccaa ctatgtttgc cacttccacg atgaagagac tttcactctt tcaaccgaca
     5881 tggcgttgct caagcgggaa gtcgagcgtt ttgaaggcaa agatggattt gatcggttct
     5941 tgtcgtttat ccaagaagcc cacagacatt acgagcttgc tgtcgttcac gtcctgcaga
     6001 agaacttccc tggcttcgca gcattcttac ggctacagtt cattggccaa atcctggctc
     6061 ttcacccctt cgagtctatc tggacaagag tttgtcgata tttcaagacc gacagattac
     6121 gaagagtctt ctcgtttgca gtgatgtaca tgggtcaaag cccatacagt gcgcccggaa
     6181 catattcctt gctccaatac accgaattga ccgagggcat ctggtatccg agaggaggct
     6241 tttggcaggt tcctaatact cttcttcaga tcgtcaagcg caacaatccc tcagccaagt
     6301 tcaatttcaa cgctccagtt tcccaggttc ttctctctcc tgccaaggac cgagcgactg
     6361 gtgttcgact tgaatccggc gaggaacatc acgccgatgt tgtgattgtc aatgctgacc
     6421 tcgtttacgc ctccgagcac ttgattcctg acgatgccag aaacaagatt ggccaactgg
     6481 gtgaagtcaa gagaagttgg tgggctgact tagttggtgg aaagaagctc aagggaagtt
     6541 gcagtagttt gagcttctac tggagcatgg accgaatcgt ggacggtctg ggcggacaca
     6601 atatcttctt ggccgaggac ttcaagggat cattcgacac aatcttcgag gagttgggtc
     6661 tcccagccga tccttccttt tacgtgaacg ttccctcgcg aatcgatcct tctgccgctc
     6721 ccgaaggcaa agatgctatc gtcattcttg tgccgtgtgg ccatatcgac gcttcgaacc
     6781 ctcaagatta caacaagctt gttgctcggg caaggaagtt tgtgatccaa acgctttccg
     6841 ccaagcttgg acttcccgac tttgaaaaaa tgattgtggc agagaaggtt cacgatgctc
     6901 cctcttggga gaaagaattc aacctcaagg acggaagcat cttgggactg gctcacaact
     6961 ttatgcaagt tcttggtttc aggccgagca ccagacatcc caagtatgac aagttgttct
     7021 ttgtcggggc ttcgactcat cccggaactg gggttcccat cgtcttggct ggagccaagt
     7081 taactgccaa ccaagttctc gaatcctttg accgatcccc agctccagat cccaatatgt
     7141 cactctccgt accatatgga aaacctctca aatcaaatgg aacgggtatc gattctcagg
     7201 tccagctgaa gttcatggat ttggagagat gggtatacct tttggtattg ttgattgggg
     7261 ccgtgatcgc tcgatccgtt ggtgttcttg ctttctgagc ggccgcgtga atttacttta
     7321 aatcttgcat ttaaataaat tttcttttta tagctttatg acttagtttc aatttatata
     7381 ctattttaat gacattttcg attcattgat tgaaagcttt gtgttttttc ttgatgcgct
     7441 attgcattgt tcttgtcttt ttcgccacat gtaatatctg tagtagatac ctgatacatt
     7501 gtggatgctg agtgaaattt tagttaataa tggaggcgct cttaataatt ttggggatat
     7561 tggctttttt ttttaaagtt tacaaatgaa ttttttccgc caggatttaa ttaacgctat
     7621 tacgccagcg gatcccgact cactataggg cgaattgggt accgggcccc ccctcgagtt
     7681 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     7741 gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     7801 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     7861 atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     7921 atgtggatga taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag
     7981 cgatgatttt tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact
     8041 aatactttca acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac
     8101 aaaaaattgt taatatacct ctatacttta acgtcaagga gaaaaaacta tagctagcat
     8161 ggaggccaag atagatgagc tgatcaataa tgatcctgtt tggtccagcc aaaatgaaag
     8221 cttgatttca aaaccttata atcacatcct tttgaaacct ggcaagaact ttagactaaa
     8281 tttaatagtt caaattaaca gagttatgaa tttgcccaaa gaccagctgg ccatagtttc
     8341 gcaaattgtt gagctcttgc ataattccag ccttttaatc gacgatatag aagataatgc
     8401 tcccttgaga aggggacaga ccacttctca cttaatcttc ggtgtaccct ccactataaa
     8461 caccgcaaat tatatgtatt tcagagccat gcaacttgta tcgcagctaa ccacaaaaga
     8521 gcctttgtat cataatttga ttacgatttt caacgaagaa ttgatcaatc tacatagggg
     8581 acaaggcttg gatatatact ggagagactt tctgcctgaa atcataccta ctcaggagat
     8641 gtatttgaat atggttatga ataaaacagg cggccttttc agattaacgt tgagactcat
     8701 ggaagcgctg tctccttcct cacaccacgg ccattcgttg gttcctttca taaatcttct
     8761 gggtattatt tatcagatta gagatgatta cttgaatttg aaagatttcc aaatgtccag
     8821 cgaaaaaggc tttgctgagg acattacaga ggggaagtta tcttttccca tcgtccacgc
     8881 ccttaacttc actaaaacga aaggtcaaac tgagcaacac aatgaaattc taagaattct
     8941 cctgttgagg acaagtgata aagatataaa actaaagctg attcaaatac tggaattcga
     9001 caccaattca ttggcctaca ccaaaaattt tattaatcaa ttagtgaata tgataaaaaa
     9061 tgataatgaa aataagtatt tacctgattt ggcttcgcat tccgacaccg ccaccaattt
     9121 acatgacgaa ttgttatata taatagacca cttatccgaa ttgtgacccg ggggcgcgcc
     9181 gaaataaatt gaattgaatt gaaatcgata gatcaatttt tttcttttct ctttccccat
     9241 cctttacgct aaaataatag tttattttat tttttgaata ttttttattt atatacgtat
     9301 atatagacta ttatttatct tttaatgatt attaagattt ttattaaaaa aaaattcgct
     9361 cctcttttaa tgcctttatg cagttttttt ttcccattcg atatttctat gttcgggttc
     9421 agcgtatttt aagtttaata actcgaaaat tctgcgttcg ttaaagctga gctcactagt
     9481 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     9541 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9601 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9661 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10381 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    10441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    11041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    11101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    11161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    11221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    11341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11701 aaaagtgcca cctgggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt
    11761 ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt
    11821 ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat
    11881 cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga
    11941 ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat
    12001 ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt
    12061 ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact
    12121 ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat
    12181 tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg tctaagaaac
    12241 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
