LOCUS       sequence-328850-       12338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses carotenoid biosynthesis gene in response to 
            2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA 
            and PhlTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12338)
  AUTHORS   Tominaga M, Nozaki K, Umeno D, Ishii J, Kondo A
  TITLE     Robust and flexible platform for directed evolution of yeast genetic
            switches.
  JOURNAL   Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y.
  PUBMED    33758180
REFERENCE   2  (bases 1 to 12338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-23"; volume: "12(1):1846. doi"; pages: " 
            10.1038/s41467-021-22134-y"
COMMENT     Sequence Label: pATP416-­ptetO7-­pphlO6-­crtYBI
FEATURES             Location/Qualifiers
     source          1..12338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2009..2025
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2010..2026
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2204..2391)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     2312..2336
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    4664..4682
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4700..4718
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4735..4753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4771..4789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4807..4825
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4842..4860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4878..4896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5005..5671
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5541..5564
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      9230..9500
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     primer_bind     complement(9565..9585)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9565..9583)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9604..9620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9604..9620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9617..9639)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9628..9644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9652..9658,9659..9676,9677..9682))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9697..9718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9835..9852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10006..10594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10086..10105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10765..11556,11557..11625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11388..11407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11626..11730)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    11767..12270
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     12311..12329
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
     2041 gaattcctgc agcccggggg atccactagt ggtaccgggc cccccctcga gttgtcctct
     2101 gaggacataa aatacacacc gagattcatc aactcattgc tggagttagc atatctacaa
     2161 ttgggtgaaa tggggagcga tttgcaggca tttgctcggc atgccggtag aggtgtggtc
     2221 aataagagcg acctcatgct atacctgaga aagcaacctg acctacagga aagagttact
     2281 caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat
     2341 tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg cggccggcct
     2401 tactgccctt cccatccgct catgaccaca ctcaagactt tccgtactct cctccatccg
     2461 gcaactgtcc ttctctctcc gacgtctcct ttccaaacga ctttgatctc tcggccgatc
     2521 agtaggtagc tcgcgcaagc cgctcgcatt cccgcttgaa cctcggtagg aagtcggtca
     2581 attcccttat aagaatgttt ggcaagatcc tctgcgtagg cgactaatgg aagcgagtac
     2641 gtcttccatt cgaaccggaa gctttctgag gcgtttgaag atggtaatgt ggacgaagga
     2701 gatagactga ggagtttgtc gaaatcttga ggccgaggtt ccgtccaatc agtcgggatc
     2761 gcaagctttg attcatcccg aagaccaaag aatgagagtg gtaggtaaaa tctcccttct
     2821 gttgcgtccc ctttaatgtc cctagcaatg ttcaccaact gaagggcagt tcccatctct
     2881 cggcttgcca ctaacacagc ttctctttct tctatggtgg cagggacctg acttggtgca
     2941 cttgcccaag agacatagac caatagctcg gcgactgagc ctgctacaca tagaccatag
     3001 tccagcaagt cagctgtggt ctcgataggc gtcttccgag cctggactgc ctctgtcgat
     3061 aaaggaaaga taagatcagt ggtgtatcct ctaaggagtt cgtcgagtgg gtatcgaggg
     3121 atcagccctt gcaacttagc gagcaacctg aaggcgaaat ggtattgaac gggaaccctt
     3181 tcggtaagga attgaacgag ctcggcaggc gagagcgaag gaggaggcgg gaggggatac
     3241 attcccgtgg gtcgggaagg gtgcgaagga ggaagtaaag gcgaagaaag gatcttgtca
     3301 ggttgcgaag ggtgtagcgg gggcccaaat agtagggtaa gaaaatcgga gaccatgtca
     3361 attgtggcat gcgggttgga agatacttca ggagagtcga taagatcatc agtcacccgg
     3421 cagaatgcgt atagtccaac cagcctctcc ctaacttcgc taggaaatcc agccgaggca
     3481 acaaaaaagc tccggctctt ttcctccaac aacttgactg ccagttccaa gtcacgtttt
     3541 ggctgagaag agtatggtcg gctgctaaaa aacagggaga gcacaggcgg tgtaatgagg
     3601 ggaaatgaag atggcatctt tttgttgcca taaatagttc gaccgtgtag caggtatagg
     3661 gcctgagtat gatcgcaggc agacagaccc agaacaatca ttagattcgt cagtaagaag
     3721 aacatagctt cctcaatggg tagtacacct ccaagcctcc accctacaat cttctcatcg
     3781 ttgatcgacc aagagtcttg accgacagca acataatcta cccaaatcag atacaccgtc
     3841 gggatcatga ttgctgcaat agttgacttt gctcggccac ttttccaatc gaaagcatat
     3901 tcgcctgata atgctgccaa gagcatggta ggtggggtga tgagtaagga gagtgcccgc
     3961 atgtagaagt agtgatctgt cacgagcggg tcgggcgatg ggctggggtg agcggtaaat
     4021 aggtagataa tgggcagagg gatgagcgcc ttgagcgcga gagaaagggc ggacgatcta
     4081 gtcttgggaa gcgcgagaga tgggagaagg tgcctagttg ccaagacgta gaccaagccg
     4141 gtgattacgg tttgaatgac aaagaaagcg tactcttcat atggaacatc tagaaacgtt
     4201 ccaaacacgc cttggccact ctccgctgat ggatatgtcc atgcgccatt tctgatgatc
     4261 catgagtccc atggtgtggt tgcactaaac gcaataaata cgaggatcga tattttgtag
     4321 atgtcaaatt ttgtcaaaat cggggaagtg agcagaccga gaagaccaag aattgggaga
     4381 gtatagatca gatggatctg gtaatatgcg agagccgtca tcctaggtat agttttttct
     4441 ccttgacgtt aaagtataga ggtatattaa caattttttg ttgatacttt tattacattt
     4501 gaataagaag taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg ttatgcagtt
     4561 tttgcattta tatatctgtt aatagatcaa aaatcatcgc ttcgctgatt aattacccca
     4621 gaaataaggc taaaaaacta atcgcattat catccacata cgttctctat cactgatagg
     4681 gagtaaactc gacatacgtt ctctatcact gatagggata aactcgacat acgttctcta
     4741 tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag taaactcgac
     4801 atacgttctc tatcactgat agggagtaaa ctcgacatcg ttctctatca ctgataggga
     4861 gtaaactcga catacgttct ctatcactga tagggagtaa actcgagggg gggcccggta
     4921 cccaattcgc cctatagtga gtcgggatcc gctggcgtaa tagcgcttaa ggaataaaaa
     4981 acacgctttt tcagttcgag tttatcatta tcaatactgc catttcaaag aatacgtaaa
     5041 taattaatag tagtgatttt cctaacttta tttagtcaaa aaattagcct tttaattctg
     5101 ctgtaacccg tacatgccca aaataggggg cgggttacac agaatatata acatcgtagg
     5161 tgtctgggtg aacagtttat tcctggcatc cactaaatat aatggagccc gctttttaag
     5221 ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat tgttttcttc accaaccatc
     5281 agttcatagg tccattctct tagcgcaact acagagaaca ggggcacaaa caggcaaaaa
     5341 acgggcacaa cctcaatgga gtgatgcaac ctgcctggag taaatgatga cacaaggcaa
     5401 ttgacccacg catgtatcta tctcattttc ttacaccttc tattaccttc tgctctctct
     5461 gatttggaaa aagctgaaaa aaaaggttga aaccagttcc ctgaaattat tcccctactt
     5521 gactaataag tatataaaga cggtaggtat tgattgtaat tctgtaaatc tatttcttaa
     5581 acttcttaaa ttctactttt atagttagtc ttttttttag ttttaaaaca ccaagaactt
     5641 agtttcgaat aaacacacat aaacaaacaa aacgcgtatg ggaaaagaac aagatcagga
     5701 taaacccaca gctatcatcg tgggatgtgg tatcggtgga atcgccactg ccgctcgtct
     5761 tgctaaagaa ggtttccagg tcacggtgtt cgagaagaac gactactccg gaggtcgatg
     5821 ctctttaatc gagcgagatg gttatcgatt cgatcagggg cccagtttgc tgctcttgcc
     5881 agatctcttc aagcagacat tcgaagattt gggagagaag atggaagatt gggtcgatct
     5941 catcaagtgt gaacccaact atgtttgcca cttccacgat gaagagactt tcactctttc
     6001 aaccgacatg gcgttgctca agcgggaagt cgagcgtttt gaaggcaaag atggatttga
     6061 tcggttcttg tcgtttatcc aagaagccca cagacattac gagcttgctg tcgttcacgt
     6121 cctgcagaag aacttccctg gcttcgcagc attcttacgg ctacagttca ttggccaaat
     6181 cctggctctt caccccttcg agtctatctg gacaagagtt tgtcgatatt tcaagaccga
     6241 cagattacga agagtcttct cgtttgcagt gatgtacatg ggtcaaagcc catacagtgc
     6301 gcccggaaca tattccttgc tccaatacac cgaattgacc gagggcatct ggtatccgag
     6361 aggaggcttt tggcaggttc ctaatactct tcttcagatc gtcaagcgca acaatccctc
     6421 agccaagttc aatttcaacg ctccagtttc ccaggttctt ctctctcctg ccaaggaccg
     6481 agcgactggt gttcgacttg aatccggcga ggaacatcac gccgatgttg tgattgtcaa
     6541 tgctgacctc gtttacgcct ccgagcactt gattcctgac gatgccagaa acaagattgg
     6601 ccaactgggt gaagtcaaga gaagttggtg ggctgactta gttggtggaa agaagctcaa
     6661 gggaagttgc agtagtttga gcttctactg gagcatggac cgaatcgtgg acggtctggg
     6721 cggacacaat atcttcttgg ccgaggactt caagggatca ttcgacacaa tcttcgagga
     6781 gttgggtctc ccagccgatc cttcctttta cgtgaacgtt ccctcgcgaa tcgatccttc
     6841 tgccgctccc gaaggcaaag atgctatcgt cattcttgtg ccgtgtggcc atatcgacgc
     6901 ttcgaaccct caagattaca acaagcttgt tgctcgggca aggaagtttg tgatccaaac
     6961 gctttccgcc aagcttggac ttcccgactt tgaaaaaatg attgtggcag agaaggttca
     7021 cgatgctccc tcttgggaga aagaattcaa cctcaaggac ggaagcatct tgggactggc
     7081 tcacaacttt atgcaagttc ttggtttcag gccgagcacc agacatccca agtatgacaa
     7141 gttgttcttt gtcggggctt cgactcatcc cggaactggg gttcccatcg tcttggctgg
     7201 agccaagtta actgccaacc aagttctcga atcctttgac cgatccccag ctccagatcc
     7261 caatatgtca ctctccgtac catatggaaa acctctcaaa tcaaatggaa cgggtatcga
     7321 ttctcaggtc cagctgaagt tcatggattt ggagagatgg gtataccttt tggtattgtt
     7381 gattggggcc gtgatcgctc gatccgttgg tgttcttgct ttctgagcgg ccgcgtgaat
     7441 ttactttaaa tcttgcattt aaataaattt tctttttata gctttatgac ttagtttcaa
     7501 tttatatact attttaatga cattttcgat tcattgattg aaagctttgt gttttttctt
     7561 gatgcgctat tgcattgttc ttgtcttttt cgccacatgt aatatctgta gtagatacct
     7621 gatacattgt ggatgctgag tgaaatttta gttaataatg gaggcgctct taataatttt
     7681 ggggatattg gctttttttt ttaaagttta caaatgaatt ttttccgcca ggatttaatt
     7741 aacgctatta cgccagcgga tccatgatac gaaacgtacc gtatcgttaa ggtgaatgat
     7801 acgaaacgta ccgtatcgtt aaggtcaatg atacgaaacg taccgtatcg ttaaggtcaa
     7861 tgatacgaaa cgtaccgtat cgttaaggtg tatgatacga aacgtaccgt atcgttaagg
     7921 tagatgatac gaaacgtacc gtatcgttaa ggtggcgttg tgatccggat gataatgcga
     7981 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
     8041 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
     8101 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
     8161 ctctatactt taacgtcaag gagaaaaaac tatagctagc atggaggcca agatagatga
     8221 gctgatcaat aatgatcctg tttggtccag ccaaaatgaa agcttgattt caaaacctta
     8281 taatcacatc cttttgaaac ctggcaagaa ctttagacta aatttaatag ttcaaattaa
     8341 cagagttatg aatttgccca aagaccagct ggccatagtt tcgcaaattg ttgagctctt
     8401 gcataattcc agccttttaa tcgacgatat agaagataat gctcccttga gaaggggaca
     8461 gaccacttct cacttaatct tcggtgtacc ctccactata aacaccgcaa attatatgta
     8521 tttcagagcc atgcaacttg tatcgcagct aaccacaaaa gagcctttgt atcataattt
     8581 gattacgatt ttcaacgaag aattgatcaa tctacatagg ggacaaggct tggatatata
     8641 ctggagagac tttctgcctg aaatcatacc tactcaggag atgtatttga atatggttat
     8701 gaataaaaca ggcggccttt tcagattaac gttgagactc atggaagcgc tgtctccttc
     8761 ctcacaccac ggccattcgt tggttccttt cataaatctt ctgggtatta tttatcagat
     8821 tagagatgat tacttgaatt tgaaagattt ccaaatgtcc agcgaaaaag gctttgctga
     8881 ggacattaca gaggggaagt tatcttttcc catcgtccac gcccttaact tcactaaaac
     8941 gaaaggtcaa actgagcaac acaatgaaat tctaagaatt ctcctgttga ggacaagtga
     9001 taaagatata aaactaaagc tgattcaaat actggaattc gacaccaatt cattggccta
     9061 caccaaaaat tttattaatc aattagtgaa tatgataaaa aatgataatg aaaataagta
     9121 tttacctgat ttggcttcgc attccgacac cgccaccaat ttacatgacg aattgttata
     9181 tataatagac cacttatccg aattgtgacc cgggggcgcg ccgaaataaa ttgaattgaa
     9241 ttgaaatcga tagatcaatt tttttctttt ctctttcccc atcctttacg ctaaaataat
     9301 agtttatttt attttttgaa tattttttat ttatatacgt atatatagac tattatttat
     9361 cttttaatga ttattaagat ttttattaaa aaaaaattcg ctcctctttt aatgccttta
     9421 tgcagttttt ttttcccatt cgatatttct atgttcgggt tcagcgtatt ttaagtttaa
     9481 taactcgaaa attctgcgtt cgttaaagct gagctcacta gttctagagc ggccgccacc
     9541 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     9601 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9661 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9721 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
    11761 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
    11821 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
    11881 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
    11941 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
    12001 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
    12061 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
    12121 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
    12181 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
    12241 gaggcgcgtg taagttacag gcaagcgatc cgtctaagaa accattatta tcatgacatt
    12301 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
