LOCUS       sequence-328856-       12210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses carotenoid biosynthesis gene in response to homoserine 
            lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and
            LuxTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Tominaga M, Nozaki K, Umeno D, Ishii J, Kondo A
  TITLE     Robust and flexible platform for directed evolution of yeast genetic
            switches.
  JOURNAL   Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y.
  PUBMED    33758180
REFERENCE   2  (bases 1 to 12210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-23"; volume: "12(1):1846. doi"; pages: " 
            10.1038/s41467-021-22134-y"
COMMENT     Sequence Label: pATP416-­pluxO5-­pphlO6-­crtYBI
FEATURES             Location/Qualifiers
     source          1..12210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2009..2025
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2010..2026
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2204..2391)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     2312..2336
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        4877..5543
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5413..5436
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      9102..9372
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     primer_bind     complement(9437..9457)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9437..9455)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9476..9492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9476..9492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9489..9511)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9500..9516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9524..9530,9531..9548,9549..9554))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9569..9590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9707..9724)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9878..10466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9958..9977)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10637..11428,11429..11497))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11260..11279
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11498..11602)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    11639..12142
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     12183..12201
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
     2041 gaattcctgc agcccggggg atccactagt ggtaccgggc cccccctcga gttgtcctct
     2101 gaggacataa aatacacacc gagattcatc aactcattgc tggagttagc atatctacaa
     2161 ttgggtgaaa tggggagcga tttgcaggca tttgctcggc atgccggtag aggtgtggtc
     2221 aataagagcg acctcatgct atacctgaga aagcaacctg acctacagga aagagttact
     2281 caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat
     2341 tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg cggccggcct
     2401 tactgccctt cccatccgct catgaccaca ctcaagactt tccgtactct cctccatccg
     2461 gcaactgtcc ttctctctcc gacgtctcct ttccaaacga ctttgatctc tcggccgatc
     2521 agtaggtagc tcgcgcaagc cgctcgcatt cccgcttgaa cctcggtagg aagtcggtca
     2581 attcccttat aagaatgttt ggcaagatcc tctgcgtagg cgactaatgg aagcgagtac
     2641 gtcttccatt cgaaccggaa gctttctgag gcgtttgaag atggtaatgt ggacgaagga
     2701 gatagactga ggagtttgtc gaaatcttga ggccgaggtt ccgtccaatc agtcgggatc
     2761 gcaagctttg attcatcccg aagaccaaag aatgagagtg gtaggtaaaa tctcccttct
     2821 gttgcgtccc ctttaatgtc cctagcaatg ttcaccaact gaagggcagt tcccatctct
     2881 cggcttgcca ctaacacagc ttctctttct tctatggtgg cagggacctg acttggtgca
     2941 cttgcccaag agacatagac caatagctcg gcgactgagc ctgctacaca tagaccatag
     3001 tccagcaagt cagctgtggt ctcgataggc gtcttccgag cctggactgc ctctgtcgat
     3061 aaaggaaaga taagatcagt ggtgtatcct ctaaggagtt cgtcgagtgg gtatcgaggg
     3121 atcagccctt gcaacttagc gagcaacctg aaggcgaaat ggtattgaac gggaaccctt
     3181 tcggtaagga attgaacgag ctcggcaggc gagagcgaag gaggaggcgg gaggggatac
     3241 attcccgtgg gtcgggaagg gtgcgaagga ggaagtaaag gcgaagaaag gatcttgtca
     3301 ggttgcgaag ggtgtagcgg gggcccaaat agtagggtaa gaaaatcgga gaccatgtca
     3361 attgtggcat gcgggttgga agatacttca ggagagtcga taagatcatc agtcacccgg
     3421 cagaatgcgt atagtccaac cagcctctcc ctaacttcgc taggaaatcc agccgaggca
     3481 acaaaaaagc tccggctctt ttcctccaac aacttgactg ccagttccaa gtcacgtttt
     3541 ggctgagaag agtatggtcg gctgctaaaa aacagggaga gcacaggcgg tgtaatgagg
     3601 ggaaatgaag atggcatctt tttgttgcca taaatagttc gaccgtgtag caggtatagg
     3661 gcctgagtat gatcgcaggc agacagaccc agaacaatca ttagattcgt cagtaagaag
     3721 aacatagctt cctcaatggg tagtacacct ccaagcctcc accctacaat cttctcatcg
     3781 ttgatcgacc aagagtcttg accgacagca acataatcta cccaaatcag atacaccgtc
     3841 gggatcatga ttgctgcaat agttgacttt gctcggccac ttttccaatc gaaagcatat
     3901 tcgcctgata atgctgccaa gagcatggta ggtggggtga tgagtaagga gagtgcccgc
     3961 atgtagaagt agtgatctgt cacgagcggg tcgggcgatg ggctggggtg agcggtaaat
     4021 aggtagataa tgggcagagg gatgagcgcc ttgagcgcga gagaaagggc ggacgatcta
     4081 gtcttgggaa gcgcgagaga tgggagaagg tgcctagttg ccaagacgta gaccaagccg
     4141 gtgattacgg tttgaatgac aaagaaagcg tactcttcat atggaacatc tagaaacgtt
     4201 ccaaacacgc cttggccact ctccgctgat ggatatgtcc atgcgccatt tctgatgatc
     4261 catgagtccc atggtgtggt tgcactaaac gcaataaata cgaggatcga tattttgtag
     4321 atgtcaaatt ttgtcaaaat cggggaagtg agcagaccga gaagaccaag aattgggaga
     4381 gtatagatca gatggatctg gtaatatgcg agagccgtca tcctaggtat agttttttct
     4441 ccttgacgtt aaagtataga ggtatattaa caattttttg ttgatacttt tattacattt
     4501 gaataagaag taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg ttatgcagtt
     4561 tttgcattta tatatctgtt aatagatcaa aaatcatcgc ttcgctgatt aattacccca
     4621 gaaataaggc taaaaaacta atcgcattat catccggatc acatacgtac ctgtacgatc
     4681 ctacaggttg acctgtacga tcctacaggt tcacctgtac gatcctacag gtggatccgc
     4741 tggcgtaata gcgacctgta cgatcctaca ggtacttatg ttcgaccttg cgtaaacctg
     4801 tacgatccta caggtactta tgttcgacct tgcgtaactt aaggaataaa aaacacgctt
     4861 tttcagttcg agtttatcat tatcaatact gccatttcaa agaatacgta aataattaat
     4921 agtagtgatt ttcctaactt tatttagtca aaaaattagc cttttaattc tgctgtaacc
     4981 cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcgta ggtgtctggg
     5041 tgaacagttt attcctggca tccactaaat ataatggagc ccgcttttta agctggcatc
     5101 cagaaaaaaa aagaatccca gcaccaaaat attgttttct tcaccaacca tcagttcata
     5161 ggtccattct cttagcgcaa ctacagagaa caggggcaca aacaggcaaa aaacgggcac
     5221 aacctcaatg gagtgatgca acctgcctgg agtaaatgat gacacaaggc aattgaccca
     5281 cgcatgtatc tatctcattt tcttacacct tctattacct tctgctctct ctgatttgga
     5341 aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac ttgactaata
     5401 agtatataaa gacggtaggt attgattgta attctgtaaa tctatttctt aaacttctta
     5461 aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac ttagtttcga
     5521 ataaacacac ataaacaaac aaaacgcgta tgggaaaaga acaagatcag gataaaccca
     5581 cagctatcat cgtgggatgt ggtatcggtg gaatcgccac tgccgctcgt cttgctaaag
     5641 aaggtttcca ggtcacggtg ttcgagaaga acgactactc cggaggtcga tgctctttaa
     5701 tcgagcgaga tggttatcga ttcgatcagg ggcccagttt gctgctcttg ccagatctct
     5761 tcaagcagac attcgaagat ttgggagaga agatggaaga ttgggtcgat ctcatcaagt
     5821 gtgaacccaa ctatgtttgc cacttccacg atgaagagac tttcactctt tcaaccgaca
     5881 tggcgttgct caagcgggaa gtcgagcgtt ttgaaggcaa agatggattt gatcggttct
     5941 tgtcgtttat ccaagaagcc cacagacatt acgagcttgc tgtcgttcac gtcctgcaga
     6001 agaacttccc tggcttcgca gcattcttac ggctacagtt cattggccaa atcctggctc
     6061 ttcacccctt cgagtctatc tggacaagag tttgtcgata tttcaagacc gacagattac
     6121 gaagagtctt ctcgtttgca gtgatgtaca tgggtcaaag cccatacagt gcgcccggaa
     6181 catattcctt gctccaatac accgaattga ccgagggcat ctggtatccg agaggaggct
     6241 tttggcaggt tcctaatact cttcttcaga tcgtcaagcg caacaatccc tcagccaagt
     6301 tcaatttcaa cgctccagtt tcccaggttc ttctctctcc tgccaaggac cgagcgactg
     6361 gtgttcgact tgaatccggc gaggaacatc acgccgatgt tgtgattgtc aatgctgacc
     6421 tcgtttacgc ctccgagcac ttgattcctg acgatgccag aaacaagatt ggccaactgg
     6481 gtgaagtcaa gagaagttgg tgggctgact tagttggtgg aaagaagctc aagggaagtt
     6541 gcagtagttt gagcttctac tggagcatgg accgaatcgt ggacggtctg ggcggacaca
     6601 atatcttctt ggccgaggac ttcaagggat cattcgacac aatcttcgag gagttgggtc
     6661 tcccagccga tccttccttt tacgtgaacg ttccctcgcg aatcgatcct tctgccgctc
     6721 ccgaaggcaa agatgctatc gtcattcttg tgccgtgtgg ccatatcgac gcttcgaacc
     6781 ctcaagatta caacaagctt gttgctcggg caaggaagtt tgtgatccaa acgctttccg
     6841 ccaagcttgg acttcccgac tttgaaaaaa tgattgtggc agagaaggtt cacgatgctc
     6901 cctcttggga gaaagaattc aacctcaagg acggaagcat cttgggactg gctcacaact
     6961 ttatgcaagt tcttggtttc aggccgagca ccagacatcc caagtatgac aagttgttct
     7021 ttgtcggggc ttcgactcat cccggaactg gggttcccat cgtcttggct ggagccaagt
     7081 taactgccaa ccaagttctc gaatcctttg accgatcccc agctccagat cccaatatgt
     7141 cactctccgt accatatgga aaacctctca aatcaaatgg aacgggtatc gattctcagg
     7201 tccagctgaa gttcatggat ttggagagat gggtatacct tttggtattg ttgattgggg
     7261 ccgtgatcgc tcgatccgtt ggtgttcttg ctttctgagc ggccgcgtga atttacttta
     7321 aatcttgcat ttaaataaat tttcttttta tagctttatg acttagtttc aatttatata
     7381 ctattttaat gacattttcg attcattgat tgaaagcttt gtgttttttc ttgatgcgct
     7441 attgcattgt tcttgtcttt ttcgccacat gtaatatctg tagtagatac ctgatacatt
     7501 gtggatgctg agtgaaattt tagttaataa tggaggcgct cttaataatt ttggggatat
     7561 tggctttttt ttttaaagtt tacaaatgaa ttttttccgc caggatttaa ttaacgctat
     7621 tacgccagcg gatccatgat acgaaacgta ccgtatcgtt aaggtgaatg atacgaaacg
     7681 taccgtatcg ttaaggtcaa tgatacgaaa cgtaccgtat cgttaaggtc aatgatacga
     7741 aacgtaccgt atcgttaagg tgtatgatac gaaacgtacc gtatcgttaa ggtagatgat
     7801 acgaaacgta ccgtatcgtt aaggtggcgt tgtgatccgg atgataatgc gattagtttt
     7861 ttagccttat ttctggggta attaatcagc gaagcgatga tttttgatct attaacagat
     7921 atataaatgc aaaaactgca taaccacttt aactaatact ttcaacattt tcggtttgta
     7981 ttacttctta ttcaaatgta ataaaagtat caacaaaaaa ttgttaatat acctctatac
     8041 tttaacgtca aggagaaaaa actatagcta gcatggaggc caagatagat gagctgatca
     8101 ataatgatcc tgtttggtcc agccaaaatg aaagcttgat ttcaaaacct tataatcaca
     8161 tccttttgaa acctggcaag aactttagac taaatttaat agttcaaatt aacagagtta
     8221 tgaatttgcc caaagaccag ctggccatag tttcgcaaat tgttgagctc ttgcataatt
     8281 ccagcctttt aatcgacgat atagaagata atgctccctt gagaagggga cagaccactt
     8341 ctcacttaat cttcggtgta ccctccacta taaacaccgc aaattatatg tatttcagag
     8401 ccatgcaact tgtatcgcag ctaaccacaa aagagccttt gtatcataat ttgattacga
     8461 ttttcaacga agaattgatc aatctacata ggggacaagg cttggatata tactggagag
     8521 actttctgcc tgaaatcata cctactcagg agatgtattt gaatatggtt atgaataaaa
     8581 caggcggcct tttcagatta acgttgagac tcatggaagc gctgtctcct tcctcacacc
     8641 acggccattc gttggttcct ttcataaatc ttctgggtat tatttatcag attagagatg
     8701 attacttgaa tttgaaagat ttccaaatgt ccagcgaaaa aggctttgct gaggacatta
     8761 cagaggggaa gttatctttt cccatcgtcc acgcccttaa cttcactaaa acgaaaggtc
     8821 aaactgagca acacaatgaa attctaagaa ttctcctgtt gaggacaagt gataaagata
     8881 taaaactaaa gctgattcaa atactggaat tcgacaccaa ttcattggcc tacaccaaaa
     8941 attttattaa tcaattagtg aatatgataa aaaatgataa tgaaaataag tatttacctg
     9001 atttggcttc gcattccgac accgccacca atttacatga cgaattgtta tatataatag
     9061 accacttatc cgaattgtga cccgggggcg cgccgaaata aattgaattg aattgaaatc
     9121 gatagatcaa tttttttctt ttctctttcc ccatccttta cgctaaaata atagtttatt
     9181 ttattttttg aatatttttt atttatatac gtatatatag actattattt atcttttaat
     9241 gattattaag atttttatta aaaaaaaatt cgctcctctt ttaatgcctt tatgcagttt
     9301 ttttttccca ttcgatattt ctatgttcgg gttcagcgta ttttaagttt aataactcga
     9361 aaattctgcg ttcgttaaag ctgagctcac tagttctaga gcggccgcca ccgcggtgga
     9421 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     9481 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9541 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9601 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9661 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9721 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9781 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9841 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9901 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9961 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10021 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10081 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10141 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10201 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10261 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    10321 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10381 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10441 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10501 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10561 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10621 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10681 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10741 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10801 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10861 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10921 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10981 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11041 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11101 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11161 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11221 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11281 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11341 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11401 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11461 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11521 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11581 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat
    11641 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat
    11701 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac
    11761 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta
    11821 atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta
    11881 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa
    11941 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct
    12001 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa
    12061 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg
    12121 tgtaagttac aggcaagcga tccgtctaag aaaccattat tatcatgaca ttaacctata
    12181 aaaataggcg tatcacgagg ccctttcgtc
//
