LOCUS       sequence-328860-        9453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human equilibrative nucleoside transporter 1 (hENT1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Tominaga M, Nozaki K, Umeno D, Ishii J, Kondo A
  TITLE     Robust and flexible platform for directed evolution of yeast genetic
            switches.
  JOURNAL   Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y.
  PUBMED    33758180
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-23"; volume: "12(1):1846. doi"; pages: " 
            10.1038/s41467-021-22134-y"
COMMENT     Sequence Label: pATP403red-hENT1
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1005..1192
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             1193..1855
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(2115..2570)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2252..2273)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2464..2483
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2696..2718
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2710..2727
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2711..2727
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2737..2756
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2737..2755
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2897..3084)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     3005..3029
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(3119..3520)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     promoter        3846..4512
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4382..4405
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      6894..7164
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     primer_bind     complement(7193..7213)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7193..7211)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7232..7248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7232..7248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7245..7267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7256..7272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7280..7286,7287..7304,7305..7310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7325..7346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7463..7480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7634..8222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7714..7733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8393..9184,9185..9253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9016..9035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9254..9358)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9426..9444
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cctattgtga gggtcagtta tttcatccag atataacccg agaggaaact
      241 tcttagcgtc tgttttcgta ccataaggca gttcatgagg tatattttcg ttattgaagc
      301 ccagctcgtg aatgcttaat gctgctgaac tggtgtccat gtcgcctagc tacgcaatct
      361 ccacaggctg caaaggtttt gtctcaagag caatgttatt gtgcaccccg taattggtca
      421 acaagtttaa tctgtgcttg tccaccagct ctgtcgtaac cttcagttca tcgactatct
      481 gaagaaattt actaggaata gtgccatggt acagcaaccg agaatggcaa tttctactcg
      541 ggttcagcaa cgctgcataa acgctgttgg tgccgtagac atattcgaag ataggattat
      601 cattcataag tttcagagca atgtccttat tctggaactt ggatttatgg ctcttttggt
      661 ttaatttcgc ctgattcttg atctccttta gcttctcgac gtgggccttt ttcttgccat
      721 atggatccgc tgcacggtcc tgttccctag catgtacgtg agcgtatttc cttttaaacc
      781 acgacgcttt gtcttcattc aacgtttccc attgtttttt tctactattg ctttgctgtg
      841 ggaaaaactt atcgaaagat gacgactttt tcttaattct cgttttaaga gcttggtgag
      901 cgctaggagt cactgccagg tatcgtttga acacggcatt agtcagggaa gtcataacac
      961 agtcctttcc cgcaattttc tttttctatt actcttggcc tcctctagta cactctatat
     1021 ttttttatgc ctcggtaatg attttcattt ttttttttcc acctagcgga tgactctttt
     1081 tttttcttag cgattggcat tatcacataa tgaattatac attatataaa gtaatgtgat
     1141 ttcttcgaag aatatactaa aaaatgagca ggcaagataa acgaaggcaa agatgacaga
     1201 gcagaaagcc ctagtaaagc gtattacaaa tgaaaccaag attcagattg cgatctcttt
     1261 aaagggtggt cccctagcga tagagcactc gatcttccca gaaaaagagg cagaagcagt
     1321 agcagaacag gccacacaat cgcaagtgat taacgtccac acaggtatag ggtttctgga
     1381 ccatatgata catgctctgg ccaagcattc cggctggtcg ctaatcgttg agtgcattgg
     1441 tgacttacac atagacgacc atcacaccac tgaagactgc gggattgctc tcggtcaagc
     1501 ttttaaagag gcgctagggg ccgtgcgtgg agtaaaaagg tttggatcag gatttgcgcc
     1561 tttggatgag gcactttcca gagcggtggt agatctttcg aacaggccgt acgcagttgt
     1621 cgaacttggt ttgcaaaggg agaaagtagg agatctctct tgcgagatga tcccgcattt
     1681 tcttgaaagc tttgcagagg ctagcagaat taccctccac gttgattgtc tgcgaggcaa
     1741 gaatgatcat caccgtagtg agagtgcgtt caaggctctt gcggttgcca taagagaagc
     1801 cacctcgccc aatggtacca acgatgttcc ctccaccaaa ggtgttctta tgtagtgaca
     1861 ccgattattt aaagctgcag catacgatat atatacatgt gtatatatgt atacctatga
     1921 atgtcagtaa gtatgtatac gaacagtatg atactgaaga tgacaaggta atgcatcatt
     1981 ctatacgtgt cattctgaac gaggcgcgct ttcctttttt ctttttgctt tttctttttt
     2041 tttctcttga actcgacgga tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     2101 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     2161 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     2221 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     2281 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     2341 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     2401 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     2461 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     2521 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     2581 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2641 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2701 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     2761 ttgggtaccg ggccccccct cgagttgtcc tctgaggaca taaaatacac accgagattc
     2821 atcaactcat tgctggagtt agcatatcta caattgggtg aaatggggag cgatttgcag
     2881 gcatttgctc ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat gctatacctg
     2941 agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac
     3001 ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa
     3061 aatcataaat cataagaaat tcgcggccgg ccgtttaaac cctaggtgta tatgagatag
     3121 ttgattgtat gcttggtata gcttgaaata ttgtgcagaa aaagaaacaa ggaagaaagg
     3181 gaacgagaac aatgacgagg aaacaaaaga ttaataattg caggtctatt tatacttgat
     3241 agcaagacag caaacttttt tttatttcaa attcaagtaa ctggaaggaa ggccgtatac
     3301 cgttgctcat tagagagtag tgtgcgtgaa tgaaggaagg aaaaagtttc gtgtgcttcg
     3361 agatacccct catcagctct ggaacaacga catctgttgg tgctgtcttt gtcgttaatt
     3421 ttttccttta gtgtcttcca tcattttttt gtcattgcgg atatggtgag acaacaacgg
     3481 gggagagaga aaagaaaaaa aaagaaaaga agttgcatgc gcctattatt acttcaatag
     3541 atggcaaatg gaaaaagggt agtgaaactt cgatatgatg atggctatca agtctagggc
     3601 tacagtatta gttcgttatg taccaccatc aatgaggcag tgtaattggt gtagtcttgt
     3661 ttagcccatt atgtcttgtc tggtatctgt tctattgtat atctcccctc cgccacctac
     3721 atgttaggga gaccaacgaa ggtattatag gaatcccgat gtatgggttt ggttgccaga
     3781 aaagaggaag tccatattgt acacccccgc gggaataaaa aacacgcttt ttcagttcga
     3841 gtttatcatt atcaatactg ccatttcaaa gaatacgtaa ataattaata gtagtgattt
     3901 tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc gtacatgccc
     3961 aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt gaacagttta
     4021 ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc agaaaaaaaa
     4081 agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag gtccattctc
     4141 ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca acctcaatgg
     4201 agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac gcatgtatct
     4261 atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa aaagctgaaa
     4321 aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa gtatataaag
     4381 acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa attctacttt
     4441 tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa taaacacaca
     4501 taaacaaaca aagtcgaatg acaaccagtc accagcctca ggacagatac aaagctgtct
     4561 ggcttatctt cttcatgctg ggtctgggaa cgctgctccc gtggaatttt ttcatgacgg
     4621 ccactcagta tttcacaaac cgcctggaca tgtcccagaa tgtgtccttg gtcactgctg
     4681 aactgagcaa ggacgcccag gcgtcagccg cccctgcagc acccttgcct gagcggaact
     4741 ctctcagtgc catcttcagc aatgtcgtga ccctatgtgc catgctgccc ctgctgttat
     4801 tcacctacct caactccttc ctgcatcaga ggatccccca gtccgtacgg atcctgggca
     4861 gcctggtggc catcctgctg gtgtttctga tcactgccat cctggtgaag gtgcagctgg
     4921 atgctctgcc cttctttgtc atcaccatga tcaagatcgt gctcattaat tcatttggtg
     4981 ccatcctgca gggcagcctg tttggtctgg ctggccttct gcctgccagc tacacggccc
     5041 ccatcatgag tggccagggc ctagcaggct tctttgcctc cgtggccatg atctgcgcta
     5101 ttgccagtgg ctcggagcta tcagaaagtg ccttcggcta ctttatcaca gcctgtgctg
     5161 ttatcatttt gaccatcatc tgttacctgg gcctgccccg cctggaattc taccgctact
     5221 accagcagct caagcttgaa ggacccgggg agcaggagac caagtcggac ctcattagca
     5281 aaggagagga gccaagagca ggcaaagagg aatctggagt ttcagtctcc aactctcagc
     5341 ccaccaatga aagccactct atcaaagcca tcctgaaaaa tatctcagtc ctggctttct
     5401 ctgtctgctt catcttcact atcaccattg ggatgtttcc agccgtgact gttgaggtca
     5461 agtccagcat cgcaggcagc agcacctggg aacgttactt cattcctgtg tcctgtttct
     5521 tgactttcaa tatctttgac tggttgggcc ggagcctcac agctgtattc atgtggcctg
     5581 ggaaggacag ccgctggctg ccaagcctgg tgctggcccg gctggtgttt gtgccactgc
     5641 tgctgctgtg caacattaag ccccgccgct acctgactgt ggtcttcgag cacgatgcct
     5701 ggttcatctt cttcatggct gcctttgcct tctccaacgg ctacctcgcc agcctctgca
     5761 tgtgcttcgg gcccaagaaa gtgaagccag ctgaggcagg gaccgcagga gccatcatgg
     5821 ccttcttcct gtgtctgggt ctggcactgg gggctgtttt ctccttcctg ttccgggcaa
     5881 ttgtgtaatc gacacgcgtg cggccgcgtg aatttacttt aaatcttgca tttaaataaa
     5941 ttttcttttt atagctttat gacttagttt caatttatat actattttaa tgacattttc
     6001 gattcattga ttgaaagctt tgtgtttttt cttgatgcgc tattgcattg ttcttgtctt
     6061 tttcgccaca tgtaatatct gtagtagata cctgatacat tgtggatgct gagtgaaatt
     6121 ttagttaata atggaggcgc tcttaataat tttggggata ttggcttttt tttttaaagt
     6181 ttacaaatga attttttccg ccaggattta attaaaaaga tgccgatttg ggcgcgaatc
     6241 ctttattttg gcttcaccct catactatta tcagggccag aaaaaggaag tgtttccctc
     6301 cttcttgaat tgatgttacc ctcataaagc acgtggcctc ttatcgagaa agaaattacc
     6361 gtcgctcgtg atttgtttgc aaaaagaaca aaactgaaaa aacccagaca cgctcgactt
     6421 cctgtcttcc tattgattgc agcttccaat ttcgtcacac aacaaggtcc tagcgacggc
     6481 tcacaggttt tgtaacaagc aatcgaaggt tctggaatgg cgggaaaggg tttagtacca
     6541 catgctatga tgcccactgt gatctccaga gcaaagttcg ttcgatcgta ctgttactct
     6601 ctctctttca aacagaattg tccgaatcgt gtgacaacaa cagcctgttc tcacacactc
     6661 ttttcttcta accaaggggg tggtttagtt tagtagaacc tcgtgaaact tacatttaca
     6721 tatatataaa cttgcataaa ttggtcaatg caagaaatac atatttggtc ttttctaatt
     6781 cgtagttttt caagttctta gatgctttct ttttctcttt tttacagatc atcaaggaag
     6841 taattatcta ctttttacaa caaatataaa accccggggg cgcgccgaaa taaattgaat
     6901 tgaattgaaa tcgatagatc aatttttttc ttttctcttt ccccatcctt tacgctaaaa
     6961 taatagttta ttttattttt tgaatatttt ttatttatat acgtatatat agactattat
     7021 ttatctttta atgattatta agatttttat taaaaaaaaa ttcgctcctc ttttaatgcc
     7081 tttatgcagt ttttttttcc cattcgatat ttctatgttc gggttcagcg tattttaagt
     7141 ttaataactc gaaaattctg cgttcgttaa agctgagctc cagcttttgt tccctttagt
     7201 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7261 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7321 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7381 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7441 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7501 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7561 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8041 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8521 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9361 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9421 ataaaaatag gcgtatcacg aggccctttc gtc
//
