LOCUS       sequence-32890-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Lowary PT, Widom J
  TITLE     New DNA sequence rules for high affinity binding to histone octamer 
            and sequence-directed nucleosome positioning.
  JOURNAL   J Mol Biol. 1998 Feb 13. 276(1):19-42.
  PUBMED    9514715
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            1998 Feb 13. 276(1):19-42."
COMMENT     Sequence Label: pGEM-3z/601
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(360..378)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(361..378)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(396..412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(396..412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(409..431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    420..436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(444..450,451..468,469..474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    489..510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(627..644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(878..897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgactcctat agggcgaatt cgagctcggt acccggggat cctctagagt gggagctcgg
       61 aacactatcc gactggcacc ggcaaggtcg ctgttcaata catgcacagg atgtatatat
      121 ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc gggggacagc
      181 gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg cctcggcacc
      241 gggattctcc agggcggccg cgtatagggt ccatcacata agggatgaac tcggtgtgaa
      301 gaatcatgct ttccttggtc attaggatcc cggacctgca ggcatgcaag cttgagtatt
      361 ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      421 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      481 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      541 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      601 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      781 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      961 tttctccctc ggaagctgcg cttcc
//
