LOCUS       sequence-328913-        8942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express miR-451 under the U6 promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pU6-mir451 EF1Alpha-puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..8942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(330..1121,1122..1190))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     953..972
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1191..1295)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1370..1389)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1561..1940
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1942..2140
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2095..2115
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2158..2338
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2385..2510
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3007..3240
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3425..3469
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3767..3884
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(4137..4170)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4174..4194
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        4504..4715
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4716..5654
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5611..5631
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5675..6271
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5675..5694)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6171..6191
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6281..6334
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6347..7045
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(7065..7098)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7154..7742
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7207..7227)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7625..7636)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7745..7761)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7746..7762)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8271..8451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(join(8513..8942,1..159))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8593..8612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
       61 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      181 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      241 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      301 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      361 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      421 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      481 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      541 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      601 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      661 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      721 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      781 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      841 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      901 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      961 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1021 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1081 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1141 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1201 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1261 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1321 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1381 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1441 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1501 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     1561 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     1621 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     1681 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     1741 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     1801 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     1861 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     1921 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     1981 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2041 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2101 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     2161 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2221 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2281 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2341 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2401 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2461 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     2521 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     2581 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     2641 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     2701 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2761 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2821 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     2881 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     2941 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     3001 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     3061 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     3121 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     3181 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     3241 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     3301 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     3361 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     3421 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     3481 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     3541 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     3601 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     3661 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     3721 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     3781 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     3841 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     3901 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gagatccgac gcgccatctc
     3961 taggcccgcg ccggccccct cgcacggact tgtgggagaa gctcggctac tcccctgccc
     4021 cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac
     4081 agataatctg ttctttttaa tactagctac attttacatg ataggcttgg atttctataa
     4141 cttcgtatag catacattat acgaagttat aaacagcaca aaaggaaact caccctaact
     4201 gtaaagtaat tgtgtgtttt gagactataa gtatcccttg gagaaccacc ttgttggaag
     4261 aagctctctg ctcagcctgt cacaacctac tgactgccag ggcacttggg aatggcaagg
     4321 aaaccgttac cattactgag tttagtaatg gtaatggttc tcttgctata cccagaaaac
     4381 gtgccaggaa gagaactcag gaccctgaag cagactactg gaagggagac tccactcgag
     4441 tactaggatc cattaggcgg ccgcgtggat aaccgtatta ccgccatgca tgtgcccgtc
     4501 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     4561 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     4621 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     4681 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     4741 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     4801 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     4861 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     4921 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     4981 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     5041 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     5101 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     5161 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     5221 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     5281 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     5341 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     5401 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgcgctttt
     5461 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     5521 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     5581 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     5641 ttcttccatt tcaggtgtcg tgagctagcc caccatgacc gagtacaagc ccacggtgcg
     5701 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     5761 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     5821 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     5881 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     5941 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6001 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6061 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6121 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6181 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6241 cacctggtgc atgacccgca agcccggtgc cggatcggga gagggcagag gaagtctgct
     6301 aacatgcggt gacgtcgagg agaatcctgg cccaccggtc gccaccagcg agctgattaa
     6361 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
     6421 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
     6481 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
     6541 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
     6601 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     6661 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     6721 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac
     6781 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
     6841 cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac ccgctaagaa
     6901 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
     6961 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
     7021 caaactgggg cacaagctta attaagaatt cgtcgaggga cctaataact tcgtatagca
     7081 tacattatac gaagttatac atgtttaagg gttccggttc cactaggtac aattcgatat
     7141 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7201 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7261 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7321 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7381 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7441 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7501 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7561 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7621 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7681 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7741 gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc acaagtagca
     7801 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     7861 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7921 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7981 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     8041 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     8101 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     8161 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     8221 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     8281 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8341 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8401 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatgtgag
     8461 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8521 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8581 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8641 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8701 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8761 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8821 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8881 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8941 gc
//
