LOCUS       sequence-328919-       11150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of DYRK3 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11150)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-DYRK3-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..11150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    158..274
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    306..326
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        326..1537
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1374..1393
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1538..1562
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3267..3291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3293..3334
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3341..4111
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(4117..4137)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    4166..4754
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4219..4239)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4637..4648)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4768..5267
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4803..4826)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5200..5219
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5288..5887
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5288..5307)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5784..5804
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6025..6258
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6330..6451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6367..6386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6421..6440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6491..6626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6646..6665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6647..6665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6675..6692)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6675..6691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6684..6706)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6833..7288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6920..6939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7130..7151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7314..7418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7419..7487,7488..8279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8450..9038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8939..8958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9192..9209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9326..9347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9362..9367,9368..9385,9386..9392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9400..9416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9405..9427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9424..9440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9424..9440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9459..9479
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9461..9479
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9507..9733
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9734..9914
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9961..10086
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10579..10812
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10997..11041
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
       61 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
      121 ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
      181 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      241 aattacaaaa acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg gaattctgca
      301 gatatcaact ttgtatagaa aagttggcct ccgcgccggg ttttggcgcc tcccgcgggc
      361 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
      421 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
      481 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
      541 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
      601 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
      661 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
      721 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
      781 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
      841 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
      901 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
      961 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     1021 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     1081 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     1141 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     1201 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     1261 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     1321 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     1381 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     1441 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     1501 cgctaaattc tggccgtttt tggctttttt gttagacaca agtttgtaca aaaaagttgg
     1561 catgaagtgg aaagagaagt tgggggatgg tgtctatgac accttcatga tgatagatga
     1621 aaccaaatgt cccccctgtt caaatgtact ctgcaatcct tctgaaccac ctccacccag
     1681 aagactaaat atgaccactg agcagtttac aggagatcat actcagcact ttttggatgg
     1741 aggtgagatg aaggtagaac agctgtttca agaatttggc aacagaaaat ccaatactat
     1801 tcagtcagat ggcatcagtg actctgaaaa atgctctcct actgtttctc agggtaaaag
     1861 ttcagattgc ttgaatacag taaaatccaa cagttcatcc aaggcaccca aagtggtgcc
     1921 tctgactcca gaacaagccc tgaagcaata taaacaccac ctcactgcct atgagaaact
     1981 ggaaataatt aattatccag aaatttactt tgtaggtcca aatgccaaga aaagacatgg
     2041 agttattggt ggtcccaata atggagggta tgatgatgca gatggggcct atattcatgt
     2101 acctcgagac catctagctt atcgatatga ggtgctgaaa attattggca aggggagttt
     2161 tgggcaggtg gccagggtct atgatcacaa acttcgacag tacgtggccc taaaaatggt
     2221 gcgcaatgag aagcgctttc atcgtcaagc agctgaggag atccggattt tggagcatct
     2281 taagaaacag gataaaactg gtagtatgaa cgttatccac atgctggaaa gtttcacatt
     2341 ccggaaccat gtttgcatgg cctttgaatt gctgagcata gacctttatg agctgattaa
     2401 aaaaaataag tttcagggtt ttagcgtcca gttggtacac aagtttgccc agtccatctt
     2461 gcaatctttg gatgccctcc acaaaaataa gattattcac tgcgatctga agccagaaaa
     2521 cattctcctg aaacaccacg ggcgcagttc aaccaaggtc attgactttg ggtccagctg
     2581 tttcgagtac cagaagctct acacatatat ccagtctcgg ttctacagag ctccagaaat
     2641 catcttagga agccgctaca gcacaccaat tgacatatgg agttttggct gcatccttgc
     2701 agaactttta acaggacagc ctctcttccc tggagaggat gaaggagacc agttggcctg
     2761 catgatggag cttctaggga tgccaccacc aaaacttctg gagcaatcca aacgtgccaa
     2821 gtactttatt aattccaagg gcataccccg ctactgctct gtgactaccc aggcagatgg
     2881 gagggttgtg cttgtggggg gtcgctcacg taggggtaaa aagcggggtc ccccaggcag
     2941 caaagactgg gggacagcac tgaaagggtg tgatgactac ttgtttatag agttcttgaa
     3001 aaggtgtctt cactgggacc cctctgcccg cttgacccca gctcaagcat taagacaccc
     3061 ttggattagc aagtctgtcc ccagacctct caccaccata gacaaggtgt cagggaaacg
     3121 ggtagttaat cctgcaagtg ctttccaggg attgggttct aagctgcctc cagttgttgg
     3181 aatagccaat aagcttaaag ctaacttaat gtcagaaacc aatggtagta tacccctatg
     3241 cagtgtattg ccaaaactga ttagctaccc aactttcttg tacaaagtgg gcggcaagcc
     3301 catccccaac cccctgctgg gcctggacag caccgctagc atcccgctgc tgaacgctaa
     3361 acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac
     3421 caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga
     3481 atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa
     3541 cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc
     3601 ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt
     3661 caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct
     3721 ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat
     3781 gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat
     3841 taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg cagccctgga
     3901 actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc tggataactt
     3961 catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg
     4021 gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg
     4081 cggagaaatc agtctgcggt ctgccgaaaa gtaagccaac tttattatac atagttgata
     4141 tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt
     4201 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4261 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4321 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4381 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4441 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4501 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4561 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4621 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4681 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4741 ggccgcctcc ccgcctggaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     4801 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     4861 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     4921 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     4981 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     5041 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     5101 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     5161 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     5221 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     5281 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5341 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5401 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5461 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5521 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5581 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5641 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5701 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5761 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5821 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5881 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     5941 atctcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6001 actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga agacaagatc
     6061 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6241 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6301 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6601 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6661 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6721 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6841 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6901 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6961 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7021 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7081 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7141 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7201 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7261 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7321 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7381 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7441 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7501 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7561 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7621 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7681 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7741 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7801 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7861 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7921 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7981 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8041 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8101 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8161 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8221 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8281 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8341 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8401 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8461 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8521 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8581 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8641 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8701 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8761 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8821 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggtgg
     8881 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8941 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9001 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9061 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9241 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9301 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9361 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9421 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     9481 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     9541 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     9601 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     9661 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     9721 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9781 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9841 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9901 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9961 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    10021 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    10081 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    10141 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10201 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10261 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10321 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10381 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
    10441 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10501 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10561 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10621 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10681 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    10741 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    10801 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    10861 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10921 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10981 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    11041 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    11101 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
//
